Báo cáo khoa học: Nuclear localization of human spermine oxidase isoforms – possible implications in drug response and disease etiology pptx
Báo cáo khoa học: Nuclear localization of human spermine oxidase isoforms – possible implications in drug response and disease etiology pptx
... catalyzes the cleavage of spermine, resulting in back-conversion to spermidine, and the generation of 3-aminopropanal and H2O2.Cellular localization of human spermine oxidase T. Murray-Stewart ... providing a linkbetween in ammation and carcinogenesis.ResultsSMO5 is expressed in human lung cell linesTo determine whether the SMO5 splice variant of the spermine oxidase gene is present in human ... components of the FAD-bindingdomain, and are therefore essential for catalysis[28,3 3–3 5].Kinetic properties of SMO5Polyamine oxidase activity of SMO5 and SMO ⁄PAOh1 was measured using increasing...
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Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx
... autolysis of mGFP–cal-pain 7–Strep by GST–IST1 and GST–MIM in vitro.Fig. S2. Intermolecular proteolysis of mGFP–cal-pain 7C290S–Strep and mGFP–calpain 7 DMITC290S – Strep by Strep–calpain 7 in ... recombinant proteins, no degraded bands wereFig. 7. Enhancement of autolysis of mGFP–calpain 7–Strep byGST–MIM in vitro. (A) Purification of mGFP–calpain 7–Strep and mGFP–calpain 7C290S–Strep ... to investigatewhether calpain 7 interacts with IST1 through MIT–MIM interactions. In this study, we examined cal-pain 7–IST1 interactions by in vitro and in vivo bindingexperiments, using...
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Tài liệu Báo cáo khoa học: Restricted localization of proline-rich membrane anchor (PRiMA) of globular form acetylcholinesterase at the neuromuscular junctions – contribution and expression from motor neurons doc
... quantitation of microgram quantities of protein uti-lizing the principle of protein-dye binding. Anal Bio-chem 72, 24 8–2 54.33 Glantz SA (1988) Primer of Biostatistics. McGraw-HillInc., NY.Proline-rich ... Sequentialactivation of butyrylcholinesterase in rostral halfsomites and acetylcholinesterase in motoneurones and myotomes preceding growth of motor axons. Develop-ment 102, 38 7–3 96.23 Layer PG (1990) Cholinesterases ... cells and ⁄ or motor neurons. In order to determine the local-ization of PRiMA-linked G4AChE, sections of tibialismuscle were triple stained for PRiMA, SV48 and AChR. The staining of PRiMA...
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Tài liệu Báo cáo khoa học: Reversible tetramerization of human TK1 to the high catalytic efficient form is induced by pyrophosphate, in addition to tripolyphosphates, or high enzyme concentration ppt
... group of thymidine, forming dTMP. Human TK1 iscytosolic and its activity is absent in resting cells, appears in late G1,increases in S phase coinciding with the increase in DNA synthesis, and disappears ... structure–function rela-tion of cytosolic human thymidine kinase. Kinetic prop-erties and oligomerization pattern of nine substitutionmutants of V106. Eur J Biochem 271, 224 8–2 256.21 Birringer ... regulation of thymidine kinase 1-likeenzymes. Structure 15, 155 5–1 566.18 Bradshaw HD Jr & Deininger PL (1984) Human thymi-dine kinase gene: molecular cloning and nucleotidesequence of a cDNA...
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Tài liệu Báo cáo khoa học: The localization of FGFR3 mutations causing thanatophoric dysplasia type I differentially affects phosphorylation, processing and ubiquitylation of the receptor pptx
... staining of 293-VnR and ATDC5 cells expressing the Y373C mutant with anti-FGFR3 and anti-phosphotyrosine sera showed bothintracellular and cell-surface phosphotyrosine staining(Figs 2Eb,c and ... 70Z-Cbl (a mutant lacking 17 amino acids in the linker and RING finger domain of c-Cbl) and wild-type or mutantFGFR3 cDNAs as indicated, then immunoprecipitated and blotted as in (B).J. Bonaventure ... Conversion of Lys650 into methionine in the TK2 domain induceshyperphosphorylation and marked intracellular retent-ion of the mutant receptor leading to phosphorylation of target signalling molecules...
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Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx
... RNA-binding activity of the human protein and also of themurine homologue was investigated further, its biologi-cal function, however, remained unclear [16,17]. Thethree-dimensional structure of ... (c.719C>T) in exon 7 and an inser-tion mutation c.61 3–6 14insA. This insertion causes aframeshift that starts at Met205 and leads to the intro-duction of a stop codon after four amino acids. Theintra-assay ... protein (AUHp40). Westernblot analysis of brain extracts consistently revealed a32 kDa AUH protein and it was thus assumed thatthe mature form of human AUH in brain has amolecular weight of...
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Tài liệu Báo cáo khoa học: High activity of human butyrylcholinesterase at low pH in the presence of excess butyrylthiocholine pptx
... have been interested in the molecularbasis of substrate activation in wild-type and mutants of human BuChE [11,15,16], as well as substrate activation in wild-type and mutants of human and Bungarus ... Protein Sci. 6,130 2–1 307.32. Main, A.R. (1979) Mode of action of anticholinesterases. Phar-macol. Ther. 6, 57 9–6 28.33. Fersht, A. (1999) Structure and Mechanism in Protein Science,pp. 17 2–1 73. ... oxyanion hole, ap-cation-binding site, and an acyl-binding pocket) are thesame in AChE and BuChE (Fig. 1). The acyl-bindingpocket, which is responsible for the difference in substratespecificity...
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Tài liệu Báo cáo khoa học: Constitutive oligomerization of human D2 dopamine receptors expressed in Spodoptera frugiperda 9 (Sf9 ) and in HEK293 cells Analysis using co-immunoprecipitation and time-resolved fluorescence resonance energy transfer pdf
... dopamine,R- (–) propylnorapomorphine, and raclopride did not affectoligomerization of D2L and D2S in Sf9 and HEK293 cells. Human D2dopamine receptors can therefore form consti-tutive oligomers in Sf9 cells and in HEK293 ... differentiation, and in ammatory and immune responses. Consequently, manyexisting therapeutic agents act by either activating orblocking GPCRs. There is now an increasing body of evidence showing that ... conformationalchanges in the proteins rather than changes in protein–protein interaction. A combination of several methodsseems therefore important for the demonstration of GPCRoligomerization.The D2dopamine...
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Báo cáo khoa học: Catalytic digestion of human tumor necrosis factor-a by antibody heavy chain pot
... happening.) Lanes 1–5 in Fig. 5A represent thebands obtained by mixing ETNF-6-H (0.1 lm) and hTNF-a (6.6 lm) during the incubation. Lanes 6–1 0show the controls. As shown in lane 1, some bandswere ... (fragment B) and SRTPS (fragment C),which are the sequences of amino acids 2–6 and 5–9 from the N-terminus of hTNF-a. This means that therecombinant hTNF-a contains impurities lacking someamino acids ... kDa (dimericform of hTNF-a), the band became faint as incubationtime increased, indicating digestion of hTNF-a.0 h47.2 h66.9 h078.5 h10 min 0 10 min 0 10 min 0 10 minA80604020002448Reaction...
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Báo cáo khoa học: Unusual stability of human neuroglobin at low pH – molecular mechanisms and biological significance docx
... Identifica-tion of native and non-native structure in kinetic fold-ing intermediates of apomyoglobin. J Mol Biol 355,13 9–1 56.25 Jamin M & Baldwin RL (1996) Refolding and unfold-ing kinetics of the ... inhibition of Ngb expression increases neuronal injury uponinduction of hypoxia, both in vitro and in vivo [1 3–1 6].Interestingly, it was shown that Ngb is expressed in astrocytes [17] and that ... denaturation of Ngb and Mb. The denatur-ation of the proteins was monitored by recording the decrease in the ellipticity at 222 nm in the far-UV CD spectra of the protein in the presence of increasing...
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