Báo cáo khoa học: Fluorescence studies of the replication initiator protein RepA in complex with operator and iteron sequences and free in solution pdf
Báo cáo khoa học: Fluorescence studies of the replication initiator protein RepA in complex with operator and iteron sequences and free in solution pdf
... found in the operator sequence. RepA contains twodomains, one of which binds specifically to the half-repeat. The other is the dimerization domain, which is involved in protein protein interactions ... nm) of free and bound unlabeled C160 RepA and wild-type RepA, with the protein concentration con-stant. The ratio of the apparent FW94 of bound and free RepA was then used to estimate the fraction ... 5393Interestingly, in the latter case, the protein binds asa monomer [2–6]. Free in solution, the protein is essentially dimeric,but it dissociates and binds as a monomer in the pres-ence of even a single iteron...
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Báo cáo khoa học: Expression studies of the core+1 protein of the hepatitis C virus 1a in mammalian cells pot
... of a protein on the basis of the presence of certain dipeptides the occurrence of which is signifi-cantly different in the unstable proteins compared with those in the stable ones [38]. The instability ... reduces the intracellular levels of the core+1 ⁄ S protein in a spe-cific and dose-dependent manner, suggesting an effect of the core protein on the translation and ⁄ or the stability of the core+1 protein. ... exclude the possibility that the decrease in core+1 ⁄ S–myc levels in the presence of core was the result of an overloading of the cellular protein- synthesis machinery and of a shortage of itscomponents....
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Tài liệu Báo cáo khoa học: Fluorescence analysis of the Hansenula polymorpha peroxisomal targeting signal-1 receptor, Pex5p pdf
... structure of the N-terminaldomain of the human homologue, solved at 2.2 A˚resolution [11]. The obtained model covers residues254–568 of HpPex5p, corresponding to the C-terminalhalf of the protein ... change of the protein. Lowering the pH to 6.0 causes a decrease in the accessibility of the emitting Trp chromophores as suggestedby the almost threefold reduction of the fraction of the exposed ... syndrome and neonatal adrenoleukodys-trophy [2–4].Pex5p binds the PTS1 of newly synthesized proteins in the cytosol and subsequently guides the cargo -protein to adocking site at the peroxisomal...
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Báo cáo khoa học: Structural studies of the capsular polysaccharide and lipopolysaccharide O-antigen of Aeromonas salmonicida strain 80204-1 produced under in vitro and in vivo growth conditions docx
... about the role of these factors in vivo and their role in furunculosis. The A-layer is believed t o play a crucial role in the bacterial protection against c omplement-mediatedkilling in vitro and ... D2O(Table 1). The NH-2a was assigned on the basis of intra-residue NOE with H-1a.TheNH-2c was assigned based on the intraresidue NOEs with H-1c,H-2c and H-3c (Fig. 3B). The presence of the intraresidue ... lLÆmin)1.An electrospray stainless steel needle (27 g) w as buttedagainst the low dead volume tee and enabled the delivery of the sheath solution t o the end of the capillary column. The separations...
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Báo cáo khoa học: Secondary transporters of the 2HCT family contain two homologous domains with inverted membrane topology and trans re-entrant loops docx
... orientations in the membrane; the N terminus of the N-terminal domainis extracellular, while the N terminus of the C-terminaldomain is intracellular. In the [st326]2HCT family,TMS I is not part of the ... 100% of the linksbetween the N-terminal sequences of ST3 with scopesubfamily, 77% with scope family, and 11% of the links with scope class (Fig. 1, NN). The distribution of the hits between the ... families and the N- and C-ter-minal halves of ST3 (open bars), between the N-terminal halves of ST3 and the C-terminal halves of ST3 (grey bars), and between the C-terminal halves of ST3 and the...
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Báo cáo khoa học: Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis Functional analysis of the promoter identifies a calcium sensitive region required for basal activity doc
... sites within the MGP coding sequence(Fig. 2 ). Analysis of the phase of each of the xMGP intronslocated within the coding region revealed that introns 2 and 3 are of phase I while intron 4 is ... human and mouseMGP genes is split into two exons (IA and IB) in the X. laevis gene, with the site of the intron insertion localizedwithin the 5¢ UTR region of t he xMGP gene (Fig. 1 and Table ... DNA-binding. In order to delineate the cis-regulatory sequences involved in mediating transcriptional activation of the xMGP gene,we engineered several promoter constructs involving 5¢ and internal...
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Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate specificity docx
... hydro-gen bonds with the base, was mutated to N in order tosee how the increased distance between the base and substrate-binding amino acid affected the binding of dThd and deoxycytidine (dCyd).ResultsWe ... A˚ in complex with the feedback inhibitor dTTP. The R-fac-tor and R free were 23.2 and 24.2%, respectively(Table 3). Most of the protein could be found in the electron-density map, with the exception of ... transition state and holding E52 in position during catalysis; and (b)investigate the amino acid residues involved in sub-strate binding (Y70 and Q81). The steady-state kinetics of Dm-dNK is compulsory...
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Tài liệu Báo cáo khoa học: Comparative studies on the functional roles of N- and C-terminal regions of molluskan and vertebrate troponin-I pdf
... TnC-bind-ing site interacts with the C-domain of TnC in both the relaxed and contractile states, which plays a role in maintaining the structural integrity of the troponin complex [17,18]. These ... probably induced by streng-thening of the interaction between the structural TnC-binding site and the C-domain of TnC accompanyingCa2+binding to site IV of TnC. In vertebrate tropo-nin, the ... com-patible with the fact that all of these proteins have atleast two of three known TnC-binding sites, namely the structural TnC-binding site, the inhibitory region, and the regulatory TnC-binding...
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Báo cáo khoa học: Functional studies of crustacean hyperglycemic hormones (CHHs) of the blue crab, Callinectes sapidus – the expression and release of CHH in eyestalk and pericardial organ in response to environmental stress pot
... hypoxia and emersion showed a 3-fold increase in glucose and a more than 30-fold increase in lactate, whereas the levels in controls remained constant. The argininekinase gene (AK) and the eukaryotic ... and identifying the native CHH neuropeptide in the ES,after the recent report of CHH cDNA from the ES[28]. Also, we examined the physiological responses of the release and expression of these two ... of the PO.Cloning and sequencing of PO-CHH cDNA The first PCR of PO cDNA with a combination of a set of primers LF1 and LR1 (Table 1) produced an ampli-con of 500 bp. On the basis of this sequence,...
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Báo cáo khoa học: NMR studies on the interaction of sugars with the C-terminal domain of an R-type lectin from the earthworm Lumbricus terrestris pot
... pseudo-threefoldsymmetry of the three subdomains designated a, b and c, and that the protein has two sugar-binding sites in subdomains a and c [24,25]. Therefore, determination of the sugar-binding ability and specificity ... (Ch; C-half) of EW29 (EW29Ch)has two sugar-binding sites in subdomains a and c, and the protein usesthese sugar-binding sites for its function as a single-domain-type hemagglu-tinin. In order ... each of the two sugar-binding sites in EW29Ch is expected toelucidate the molecular basis of the carbohydratecross-linking properties of the lectin. In this study, the sugar-binding ability and...
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Từ khóa: tài liệu báo cáo khoa học bản chất của khủng hoảng kinh tế thế giới pdflam the nao de tom tat bao cáo khoa hocbáo cáo khoa họcbáo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngTìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Tranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)chuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲ