Báo cáo khoa học: Protein transport in organelles: The composition, function and regulation of the Tic complex in chloroplast protein import pptx
Báo cáo khoa học: Protein transport in organelles: The composition, function and regulation of the Tic complex in chloroplast protein import pptx
... stabilization of the Toc /Tic/ preprotein supercomplex. In this model, Tic1 10 forms the channel protein and also acts in the recruitment of Hsp93 in concertwith the co-chaperone Tic4 0. The TPR domain of Tic4 0 ... 1166–1176 ª 2009 The Authors Journal compilation ª 2009 FEBSMINIREVIEW Protein transport in organelles: The composition, function and regulation of the Tic complex in chloroplast protein import J. ... patch on the protein surface, located in the N-terminal half of the protein, including the dehydrogenase domain. Spe-cific binding of the FNR is mediated by a unique series of proline/serine-rich...
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Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx
... monitored by the reduction of cytochrome c in the presence of NADH at 550 nm [1]. Protein determination Protein content was determined by the Bradford test [20],using bovine serum albumin as standard.PHI ... Lovenberg [23] and Beinert [24], respectively.Kinetic constants The catalytic activity of PHR was evaluated both in the presence and in the absence of PHI.Km and kcatwere determined from Hanes–Haldane ... in Km and kcatarenot significant, suggesting that the catalytic activity of PHRdoes not depend on the presence of the regulatory protein. Fig. 2. MALDI-TOF spectrum of PHI. The protein was...
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Tài liệu Báo cáo khoa học: Hepatic stimulator substance mitigates hepatic cell injury through suppression of the mitochondrial permeability transition pdf
... cloning the PCR fragments into the His-tag vector, pET-15b. The recombinant proteins were generated in the E. coli strainOrigami (DE3), and the N-terminal His-tagged proteinswere purified using ... opening and cell swelling, an event thatis probably reduced in extent by CsA. CsA specificallyinhibits PTP opening by binding to cyclophilin D in the matrix and on the inner surface of the IM ... whetherHSS could function as an MPT inhibitor, thereby alle-viating hepatic injury and promoting the survival of hepatocytes, after transfection of HSS into the cells or the administration of HSS to...
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Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): reactivity and structure of metal–thiolate clusters* doc
... located in independent domains(a b-domain for the N-terminal cluster and ana-domain for the C-terminal cluster). In the absence of metals, apo-thionein (apo-T) is predominantly unstruc-tured and ... composed of a single polypep-tide chain of 61–68 residues. They are characterized bya conserved array of 20 cysteines and the absence of His and aromatic amino acids. MT3 contains 68amino acids ... asa result of the formation of disulfide bridges [2]. Sec-ond, in contrast to the high thermodynamic stability, the binding is kinetically labile, allowing rapid intra-molecular and intermolecular...
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Tài liệu Báo cáo khoa học: Arabidopsis thaliana BTB⁄ POZ-MATH proteins interact with members of the ERF⁄AP2 transcription factor family ppt
... forming eight b-sheets [23]. The motifwas noted on the basis of homology with the C-terminalregion of meprins A and B and the TRAF-C domain, and, like the BTB domain, facilitates protein protein interaction ... withCUL3 and other BTB proteins [2,16], it remains unclearwhat kind of interactions are mediated by the MATHdomain in plants. In this article, we show that the MATH domain of BPM proteins is ... proteins use their BTB ⁄ POZ domain to interactwith the cullins CUL3A and CUL3B [3,4,16], we inves-tigated what kind of protein protein interactions werefacilitated by their MATH domains by...
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Tài liệu Báo cáo khoa học: "Collecting a Why-question corpus for development and evaluation of an automatic QA-system" pdf
... answers each. The total cost of producing the corpus was about $350, consisting of $310 paid in workers rewards and $40 in Mechanical Turk fees,including all the trials conducted during the devel-opment ... documents). The source of Wik-iXML is the English part of the Wikipedia XMLCorpus by Denoyer and Gallinari (2006). In the original data some of the HTML-structures like lists and tables occurred within ... the moon in the rare earth hypothesis?Rephrased Q 4 Why is the moon so important in the rare earth hypothesis?Rephrased Q 5 What makes the moon necessary, in regards to the rare earth hypothesis?Answer...
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Báo cáo khoa học: MicroRNA-9 inhibits ovarian cancer cell growth through regulation of NF-jB1 ppt
... suppression of malignantphenotypes, suggesting the role of miR-9 in the growthinhibition of malignant cells. The fundamental function of miRNA is to regulatetheir targets by direct cleavage of the ... of tumorigenesis, and may have diagnostic and therapeutic value in the future.Materials and methodsMaterialsFour pairs of ovarian tissues, including four human ovariancancer tissues and the ... p100(NF-jB2), and different NF-jB complexes are formedfrom their homodimers and heterodimers. In most celltypes, NF-jB complexes are retained in the cytoplasmby a family of inhibitory proteins known...
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Báo cáo khoa học: A new approach for distinguishing cathepsin E and D activity in antigen-processing organelles pdf
... organelles.Therefore it could be used to study the involvement of these proteinases in protein degradation and the processing of invariant chain. An assay combi-ning a new monospecific CatE antibody and ... distinguishes between the activities of the enzymatically similar proteinases, CatE and CatD, and can therefore be used to investigate the involvement of these enzymes in antigen processing and ... Other studies have util-ized a specific inhibitor of CatE, the Ascaris pepsininhibitor, which inhibits pepsins and CatE [33], butdoes not affect other types of aspartic proteinasesincluding...
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Tài liệu Báo cáo khoa học: Loose interaction between glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase revealed by fluorescence resonance energy transfer–fluorescence lifetime imaging microscopy in living cells doc
... upon the distance between and relative ori-entation of two fluorophores, being independent of the strength of protein protein interactions. In the case of very weak interactions as described in ... citrine) and citrine–GAPDH(chimeric protein with N-terminal citrine and C-terminalGAPDH) were localized in the cytoplasm. PGK–ceru-lean (chimeric protein with N-terminal PGK and C-terminal ... technique for the observation of protein protein interactions [14,15]. This method can be applied tointeractions in loose complexes in living cells, and hasan advantage in that the FRET strength...
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Tài liệu Báo cáo khoa học: Hypoxia downregulates farnesoid X receptor via a hypoxia-inducible factor-independent but p38 mitogen-activated protein kinase-dependent pathway doc
... cholesterol, and proteins, isthen stored in the gall bladder. Bile acids excreted intobile undergo intensive enterohepatic circulation. Theyare reabsorbed in the intestine, taken up again by the liver, ... proteins,promoting binding of the von Hippel–Lindau tumorsuppressor protein, which acts as the ubiquitin ligasefor HIF-a proteins, leading to the degradation by the proteasome [20]. Any HIF-a escaping ... understanding of the molecular basis of cholestasis progressing under ischemia. Furtherinsights into the crosstalk between p38 MAPK and FXR will be useful in identifying a novel therapeutictarget...
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