Báo cáo khoa học: SAF-3, a novel splice variant of the SAF-1/MAZ/Pur-1 family, is expressed during inflammation pptx
Báo cáo khoa học: SAF-3, a novel splice variant of the SAF-1/MAZ/Pur-1 family, is expressed during inflammation pptx
... variant of the SAF-1/MAZ/Pur-1 family, is expressed during inflammation Alpana Ray1, Srijita Dhar1, Arvind Shakya1, Papiya Ray1, Yasunori Okada2and Bimal K. Ray11 Department of Veterinary ... CCATTCCAGgtgagtag 84 ctccgcagGCCGCGCCG2 851 CTTCTCCCGgtgtgcac 403 gtccccagGCCGGATCA364AATGTGAGgtaggaag 277 ctcctcagAAATGTGAG4 172 CAACAAAGgtacatgc 1335 ctgtgcagGTACTGGTG5 1028 A. Ray et al. ... cytokineand growth factor-induced cells as well of mRNAs isolated from severaldiseased tissues revealed abundant expression of SAF-3. The transactiva-tion potential of SAF-3 is much greater than...
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Báo cáo khoa học: Phaiodotoxin, a novel structural class of insect-toxin isolated from the venom of the Mexican scorpion Anuroctonus phaiodactylus pdf
... tran-scriptase. The cDNA was joined with the a daptor providedby the kit (5 ¢-gcugauggcgaugaaugaacacugcguuugCUGGCUUUGAUGAAA-3¢) using T4 DNA ligase. The modi-fied cDNA was used as t emplate for PCR amplification.Two ... scorpions of the familyButhidae, probably because they are dangerous to humans. A large number of different protein and polypeptides havebeen isolated and c haracterized from this family. Among the ... Phaiodotoxin, a novel structural class of insect-toxin isolated from the venom of the Mexican scorpionAnuroctonus phaiodactylusNorma A. Valdez-Cruz1, Cesar V. F. Batista1, Fernando Z. Zamudio1,...
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Tài liệu Báo cáo khoa học: TransLISA, a novel quantitative, nonradioactive assay for transcription factor DNA-binding analyses pdf
... hD1 hAutoradiographyAdd D beads and incubateD A O2Read AlphaLISA signal at 615 nmHSEFig. 1. Comparison of EMSA and TransLISA for the detection of HSF1–DNA binding activity. (A) Schematic ... between the addition of reagents. The broad analytical range of the assay allows quanti-tation of large differences in the DNA-binding activity of transcription factors. This is precluded in EMSAanalysis, ... temperature.Assay procedure The assay was run as a three-step assay: initial incubation of the sample and probe, addition and incubation of the sampleand acceptor beads in the plate wells, and addition...
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Tài liệu Báo cáo khóa học: TbPDE1, a novel class I phosphodiesterase of Trypanosoma brucei pdf
... 5¢-GGGAATTCCATATGAGAGACAATATTTCCCGTTTATCAAATC-3¢ and 5¢-CCGCTCGAGTCATTACTAGGTTCCCTGTCCAGTGTTACC-3¢,andPDE1(Lys321–Thr620) was amplified with primers 5¢-GGGAATTCCATATGAAGAATGATCAATCTGGCTGCGGCGCAC-3¢ and 5¢-CCGCTCGAGTCATTACTAGGTTCCCTGTCCAGTGTTACC-3¢. ... explore the PDEs of parasites as potentialtargets for antiparasitic drugs. The African trypanosomeTrypanosoma brucei is the protozoon that causes the fatalhuman sleeping sickness, as well as Nagana, ... concentration, a cAMP is the total activity used in the enymatic reaction, a probe is the total radioactivity on the filter, and qcAMPand qAMPare the precipitation efficiencies of cAMP and AMP,...
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Báo cáo khoa học: Purification and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot
... GGT AGC CAA 27693QEGYGSTQLPSDEPLNGLNDCAA GAA GGA TAT GGC AGC ACC CAA CTT CCA TCG GAC GAG CCA TTG AAC GGG CTC AAC GAC 336113KAIQDILNEHNMFRAKERVPAAG GCC ATA CAG GAC ATC CTC AAC GAG CAC AAC ATG ... 15653QEAVEGAEQVASESASLTTPCAG GAG GCG GTA GAG GGC GCC GAG CAG GTA GCG TCC GAG TCA GCC TCC CTC ACA ACA CCA 21673TTREEQPAAEAAASTAGGSQACC ACC AGG GAA GAA CAA CCG GCG GCA GAG GCC GCG GCG TCC ACC GCT ... Kurosaka A, Yano A, Itoh N, Kuroda Y, Nakagawa T& Kawasaki T (1991) The structure of a neural specificcarbohydrate epitope of horseradish peroxidase recog-nized by anti-horseradish peroxidase...
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Báo cáo khoa học: Brox, a novel farnesylated Bro1 domain-containing protein that associates with charged multivesicular body protein 4 (CHMP4) potx
... has a pointmutation at amino acid 408, was c reated by PCR-basedsite-directed mutagenesis using pmGFP–Brox as a template and complementary primers (5¢-CAA AAGGAC ACT GGG TCC TAC ATC TCC TAA ... 52 789 4088E-mail: mmaki@agr.nagoya-u.ac.jpDatabase The nucleotide sequence of the human BroxcDNA is available in the DDBJ ⁄ EMBL ⁄ Gen-Bank database under accession numberAB276123(Received ... Peroxidase-conju-gated goat anti-rabbit IgG and goat anti-mouse IgG wereobtained from Wako (Osaka, Japan). Preparation of rabbitpAbs against Alix has been described previously [40].Cy3-labeled anti-mouse...
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Báo cáo khoa học: Molecular cloning and functional expression of the human sodium channel b1B subunit, a novel splicing variant of the b1 subunit potx
... the same splicing variant from human tissue. Here, we report the cloning and characterization of a novel, splicing variant of the human b1subunit by rapid amplification of cDNA end polymerase chain ... shown are the mean (j) and standard deviation (bars) for each cRNA a: b ratio, and the sample size percRNA combination is shown in parenthesis. Data are from five different batches of oocytes, each ... MW.Custom-made software and hardware were used for acquisition andanalysis of data. Leakage and linear capacity currents weresubtracted by using P/4 protocols. Data were sampled onceevery 5 ls and...
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Báo cáo khoa học: Plasmoredoxin, a novel redox-active protein unique for malarial parasites doc
... antioxidant proteinthat is also involved in DNA synthesis may represent a potential drug target.Acknowledgements The technical assistance of Elisabeth Fischer, Petra Harwaldt and BeateHecker is acknowledged. ... the His-tagand 22 kDa) appeared when probing the recombinantprotein and a trophozoite extract of P. falciparum.Thisresult, and the fact that PfPlrx was amplified from a cDNAlibrary, indicate ... malaria; Plasmodium falciparum;redox-metabolism; thioredoxin superfamily. The malarial parasite, Plasmodium falciparum is respon-sible for more than 2 million deaths per year and novel antiparasitic...
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Báo cáo khoa học: "Towards a Semantic Classification of Spanish Verbs Based on Subcategorisation Information" doc
... are the 3-way and 15-way classifica-tions. The baseline is calculated, for each task, as the average value of the Adjusted Rand measure for100 random cluster assignations. Although all the tasks ... inevaluating unsupervised classification tasks against a gold standard lies in the fact that the class labels of the obtained clusters are unknown. Therefore, the evaluation is done according to the ... spite of these results, a qualita-tive observation of the output clusters reveals thatthey are intuitively plausible, and that the evalua-tion is penalised by the fact that the target classesare...
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Báo cáo khoa học: Broad antibiotic resistance profile of the subclass B3 metallo-b-lactamase GOB-1, a di-zinc enzyme potx
... reverse:(5¢-GATCTTGCTGCTTACTGCGGCTCACTACGACCATACAGG-3¢)(5¢-GCACCTGTATGGTCGTAGTGAGCCGCAGTAAGCAGC-3¢)For the Q116N mutant forward and reverse:(5¢-GATCTTGCTGCTTACTAACGCTCACTACGACCATACAGG-3¢)(5¢-GCACCTGTATGGTCGTAGTGAGCGTTAGTAAGCAGC-3¢)For ... reverse:(5¢-GATCTTGCTGCTTACTAACGCTCACTACGACCATACAGG-3¢)(5¢-GCACCTGTATGGTCGTAGTGAGCGTTAGTAAGCAGC-3¢)For the Q116H mutant forward and reverse:(5¢-GATCTTGCTGCTTACTCATGCTCACTACGACCATACAGG-3¢)(5¢-GCACCTGTATGGTCGTAGTGAGCATGAGTAAGCAGC-3¢)Production and ... substrate. In these cases, the kcatvalues were obtained frominitial hydrolysis rates measured at saturating substrateconcentrations. All data were analysed using MicrosoftExcel and the kaleidagraph...
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