Báo cáo khoa học: Nanoparticles can induce changes in the intracellular metabolism of lipids without compromising cellular viability docx

... (2009) 6204–6217 ª 2009 The Authors Journal compilation ª 2009 FEBS 6217 Nanoparticles can induce changes in the intracellular metabolism of lipids without compromising cellular viability Ewa Przybytkowski, ... NPs can affect the intracellular metabolism of lipids and induce HIF-1a-mediated signaling. The results suggest that QDs, together with trophic factors,promote the accumulation of lipids in cytoplasmicLDs, ... HIF-1ais involved directly in the accumulation of lipids trig-gered by QDs under the conditions of uncompromisedcell survival in the presence of serum.On the basis of the results obtained in this...

Báo cáo khoa học: C ﬁ G base mutations in the CArG box of c-fos serum response element alter its bending ﬂexibility Consequences for core-SRF recognition potx

... provide information on the localrepartition of the angles involved in the SREfoshelixbending.Relative effect of bending strainThere are several indications of a redistribution of the strains ... Indeed, the change in the array of the hydrogen bonds at thymine of SREGGfosis probably a sign of perturbation in the hydration scheme along the minor groove of the (A ⁄ T)domain. The G–C ... the HMG box. Otherwise, the temperature-induced struc-tural changes in the Raman spectra during premeltingare mainly characterized, in SVD analysis, by variation in the V2contribution of the spectral...

Tài liệu Báo cáo khoa học: KCNE4 can co-associate with the IKs (KCNQ1–KCNE1) channel complex ppt

... 7. KCNE trafficking in the presence of KCNQ1, and KCNQ1trafficking in the presence of multiple KCNE proteins. Representa-tive western blots examining KCNE protein trafficking to the plasma membrane ... 6), indicating that impaired KCNQ1cell surface expression cannot explain the suppression of IKsby KCNE4.Next, we examined the ability of KCNE proteins totraffic to the plasma membrane in the ... reaches the cell surface in the presence of KCNQ1. Multiplebands representing the differentially glycosylated forms of KCNE1 were detected, indicating normal matura-tion of the protein. Figure...

Tài liệu Báo cáo khoa học: Moult cycle-related changes in biological activity of moult-inhibiting hormone (MIH) and crustacean hyperglycaemic hormone (CHH) in the crab, Carcinus maenas From target to transcript ppt

... biological activity of MIH and CHHduring precisely timed stages of the moult cycle of Carcinusto determine changes in: (a) potency of these peptides in repressing ecdysteroid synthesis; (b) receptor ... level. In view of these observations, we also investigated levels of MIH and CHH peptides in the sinus gland and levels of transcripts in XO neurones during the moult cycle, to seewhether signiﬁcant ... Kawakami,T., Aimoto, S. & Sonobe, H. (2000) Changes in the amounts of the molt-inhibiting hormone in sinus glands during the molt cycle of the American crayﬁsh, Procambarus clarkii. Zool. Sci....

Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf

... allows the estimation of the effect of intracellular binding on the MDR phenomenon. The data detailed obtained in the present study indicatethat innate cellular MDR interferes successfully with the ... of rhodamine dyes into cells is tofollow their quenching upon entering the cells. In respiring cells, the active concentration of these dyes in the mitochondria results in quenching of their fluores-cence. ... nm. Inhibition of the NBD-Cl-sensitive efflux allowed the uptake of TMRM and its quenching within the cells. Right: the various cell linesindicated in the figure were incubated for 15 min with the...

Tài liệu Báo cáo khoa học: Ionic strength and magnesium affect the speciﬁcity of Escherichia coli and human 8-oxoguanine-DNA glycosylases pdf

... address the in uence of the reaction conditions on the binding of Fpg and OGG1to damaged DNA, we determined Kdvalues for bindingunder the same conditions as used for the kinetic experi-ments. In ... Polyamines, crowding agents,biotin and caffeine affected the activity and speciﬁcity of Fpg or OGG1only marginally. We conclude that, in the intracellular environment, the speciﬁcity of Fpg ... incorporation of 8-oxoG into DNA during replication [7–9]. Inacti-vation of the GO system increases the mutagenesis rate in bacteria [11] and increases the risk of cancer devel-opment in mouse models...

Tài liệu Báo cáo khoa học: Domain deletions and substitutions in the modular protein evolution doc

... domain deletions are more com-mon at either of the protein termini, and that theiroccurrence is slightly higher at one of the termini,depending on the number of domains in the proteinand the ... C−terminusLength of the N terminus in % of the deleted domainLength of the C terminus in % of the deleted domainPfam, N−terminus Pfam, C −terminusNumber of occurencesNumber of occurences2500035000550000 ... size of the deleted domain was calculated for the proteins with the complete arrangement. To take into account the variability of the length of the complete domain, the length of the N-terminal...

Tài liệu Báo cáo khoa học: Tryptophan tryptophylquinone cofactor biogenesis in the aromatic amine dehydrogenase of Alcaligenes faecalis Cofactor assembly and catalytic properties of recombinant enzyme expressed in Paracoccus denitriﬁcans pptx

... substrate carbinolamine intermediate;III, iminoquinone intermediate; IV, product Schiff base intermediate;V, aminoquinol intermediate. In the oxidative half-reaction the ami-noquinol intermediate ... substrates. The lack of inhibition observed with benzylamine (Fig. 5A) in comparison to the inhibition observed with tryptamine(Fig. 5B) suggests differences in binding of the twosubstrates within the ... kinetically in uentialionizations were identiﬁed in plots of klim⁄ Kd of pKavalues 7.1 and 9.3,again with the maximum value realized in the alkaline region. The poten-tial origin of these kinetically...

Tài liệu Báo cáo khoa học: "Enhanced word decomposition by calibrating the decision threshold of probabilistic models and using a model ensemble" pdf

... Another way of classifying ap-proaches is based on the learning aspect during the construction of the morphological model. If the data for training the model has the same struc-ture as the ... cross-validation on datasets ranging from 500 to 2500words with the goal of measuring how the learningimproves with increasing experience in terms of training set size. We want to remind the reader thatour ... Nevertheless, across the entirePR curve none of the algorithms dominates. Onecurve would dominate another if all data points of the dominated curve were beneath or equalto the dominating one....

Tài liệu Báo cáo khoa học: "Identifying Agreement and Disagreement in Conversational Speech: Use of Bayesian Networks to Model Pragmatic Dependencies" docx

... testing and the rest of the data fortraining. Table 7 summarizes the performance of our classifier with the different feature sets in thisclassification task, distinguishing the case where the four ... other speakerseconds of silence during the spurtspeech rate in the spurtLexical features:number of words in the spurtnumber of content words in the spurtperplexity of the spurt with respect ... con-firmed in 22.5% of the cases, while the probabil-ity of the agreement class is only 18.8%. The onlyslightly surprising result appears in the last column of the table, from which we cannot conclude...

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