... in the absence of the other substrate. In the presence of oxoglutarate,however, coenzyme binding was tighter and thereforemeasurable and, in particular, gave measurable Kdvalues for binding ... evolution of nucleotide-binding protein.Nature 250, 194–199.5 Wierenga RK, Terpstra P & Hol WG (1986) Prediction of the occurrence of the ADP-binding bab-fold in pro-teins, using an amino acid ... 243(E243) in the adenine ribose-binding pocket. We have examined the possi-bility of altering coenzyme specificity of the Peptostreptococcus enzyme,and, more specifically, the role of residue...