Báo cáo khoa học: Comparing the substrate speciﬁcities of cytochrome c biogenesis Systems I and II docx

... ResC CcdACcmHCcmGDsbDDCcmBCcmACcmCp-sidep-sideDisulfide isomerizationFig. 1. Schematic representation of cytochrome c biogenesis Systems I and II. The systems illustrated are System I ... staining of the gel, of periplasmicfractions from cells expressing P. denitriﬁcans cytochrome c 550 and the indicated biogenesis system (I or II) . The periplasmic fractionfrom cells expressing ... which is consistent with the amount of cytochrome determined spectroscopically comparedwith System I. The a-band of the pyridine hemo-chrome spectrum, which is indicative of the saturationof...

Báo cáo khoa học: Probing the substrate speciﬁcities of matriptase, matriptase-2, hepsin and DESC1 with internally quenched ﬂuorescent peptides potx

... a1-antitrypsin; a1-ACT, a1-antichymotrypsin; AT III, antithrombin III; PAI-1, palsminogen activator inhibitor I; a2-AP, a2-antiplasmin.Inhibitor RCL P4–P4¢Concentration(nM)Residual activity ... chemical and physiological inhibitors. In a side-by-side comparison,we ﬁnd that these TTSPs exhibit speci c and distinctbiochemical and enzymatic properties.ResultsExpression, puriﬁcation and characterization of ... conditions for matriptase-2. Athigh substrate concentrations, the initial velocity decreasedas a function of increasing substrate concentration, indicat-ing that there was some substrate inhibition....

Báo cáo khoa học: Predicting the substrate speciﬁcity of a glycosyltransferase implicated in the production of phenolic volatiles in tomato fruit pptx

... aninteraction between Tyr315 and the main-chain atoms of Ser14 and Pro15 anchors this loop within the vicinity of the active site, therefore signiﬁcantly reducing the size and accessibility of the acceptor ... this induction coincides with the expression of pathogen-related proteins and sali-cylic acid synthesis during hypersensitive responseinitiation [31].Recombinant enzyme activity The mapping and ... between the conformation clusters lies in the position of the aromatic ring of salicyl alcohol in the binding pocket. In clusters 1 and 3, the ring is oriented‘inside’, towards the conserved core of...

Báo cáo khoa học: Differences in substrate speciﬁcities between cysteine protease CPB isoforms of Leishmania mexicana are mediated by a few amino acid changes potx

... associated with the catalytic triad (L. mexicana residuesCys25, His163 and Asn183; Fig. 4) but the substrate specificity is defined by the bindin g affinities of the subsites. The c atalytic residues ... S1¢-S3¢subsite specificities determined using a series of fluorogenicpeptide derivatives in which substitutions were made onpositions P3to P3¢ by natural amino acids. Carboxydipep-tidase act ivities ... explore the effects on substrate utilization of the r estricted l ocal amino acid variations of the CPB is oen-zymes of L. mexicana, the recombinant CPB3 ( rCPB3), and arecombinantH84YmutantofCPB3thatwasgenerated,were...

Báo cáo khoa học: Molecular basis for speciﬁcities of reactivating factors for adenosylcobalamin-dependent diol and glycerol dehydratases pptx

... reactivating factors in the activation of inactive enzyme–CN-Cbl complex The specificities of reactivating factors in the in vitroactivation of inactive enzyme–CN-Cbl complexes werestudied similarly ... this conclusion. Theseresults are described here.Results Specificities of reactivating factors in the reactivation of inactivated holoenzymes The specificities of reactivating factors in the in ... adenosyl radical, a catalytic radi-cal in the active site, by homolysis of the coenzymeCo -C bond and catalyze the reactions by utilizing the high reactivity of the radical. The catalytic and inter-mediate...

Báo cáo khoa học: pH dependence, substrate speciﬁcity and inhibition of human kynurenine aminotransferase I ppt

... hKAT -I, and also provides biochemical information about the s ubstratespeciﬁcity and cosubstrate inhibition of the enzyme. hKAT -I is inhibited by Tris under physiological pH conditions, w hichexplains ... (5¢-CTCGAGATGGCCAAACAGCTGCAG) and reverse primer (5-AAGCTTAGAGTTCCACCTTCCACTT) containing a XhoIandaHindIII restrictionsite (underlined sequence), respectively. The P CR productswere cloned into a TOPO ... Biomedical Sciences, University of Wisconsin(Madison, WI, USA) and Dr Menico Rizzi (De partment of G enetics,University of Pavia, Italy) f or their crit ical reading of the manus cript.This study...

Báo cáo khoa học: "Comparing the Accuracy of CCG and Penn Treebank Parsers" docx

... formor another; the interesting question is whether any-thing is gained by converting the PTB into CCG.2 The comparison focuses on accuracy and is per-formed by converting CCG derivations into ... task.There were two approaches we considered for the conversion. One possibility is to associate PTBtree structures with CCG lexical categories, and combine the trees together in step with the cate-gory ... cate-gory combinations in a CCG derivation — in much the same way that an LTAG has elementary treesin the lexicon which are combined using the sub-stitution and adjunction rules of TAG. The secondapproach...

Tài liệu Báo cáo khoa học: "Bucking the Trend: Large-Scale Cost-Focused Active Learning for Statistical Machine Translation" docx

... Haffari and Anoop Sarkar. 2009. Activelearning for multilingual statistical machine trans-lation. In Proceedings of the Joint Conference of the 47th Annual Meeting of the ACL and the 4th In-ternational ... canindeed buck the trend of diminishing returns,achieving an order of magnitude increase in the rate of improvement in performance.Section 2 discusses related work; Section 3discusses preliminary ... pages135–139, Athens, Greece, March. Association forComputational Linguistics.Francois Mairesse, Milica Gasic, Filip Jurcicek, SimonKeizer, Jorge Prombonas, Blaise Thomson, Kai Yu, and Steve Young....

Tài liệu Báo cáo khoa học: "Bridging the Gap Between Underspeciﬁcation Formalisms: Minimal Recursion Semantics as Dominance Constraints" docx

... ϕ and a root of ϕ otherwise.Definition 3. A dominance constraint ϕ is normal (and compact) if it satisfies the following conditions.N1 (a) each variable of ϕ occurs at most once in the labeling ... normal dominance constraints(Koller et al., 2003) do not apply.2 Minimal Recursion SemanticsWe define a simplified version of Minimal Recur-sion Semantics and discuss differences to the origi-nal ... Bridging the Gap Between Underspecification Formalisms:Minimal Recursion Semantics as Dominance ConstraintsJoachim NiehrenProgramming Systems LabUniversität des Saarlandesniehren@ps.uni-sb.deStefan...

Tài liệu Báo cáo khoa học: A single amino acid substitution of Leu130Ile in snake DNases I contributes to the acquisition of thermal stability A clue to the molecular evolutionary mechanism from cold-blooded to warm-blooded vertebrates docx

... amino acid substitution, Leu130Ile,might be involved in an evolutionally critical change in the thermal stabilities of vertebrate DNases I. AmphibianDNasesIhaveaSer205insertioninaCa2+-binding ... hadsignificantly low serum DNase I activity levels [13]. Thesefindings indicate that the thermal stabilities of DNase I in vitro might reflect the enzyme activities in vivo.Wefound that amphibian ... DNases I are characterized by a C- terminal end with a unique cysteine-rich stretch and byinsertion of a Ser residue into the Ca2+-binding site,resulting in thermal instability compared with...

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