Báo cáo khoa học: Roles of the SH2 and SH3 domains in the regulation of neuronal Src kinase functions pptx

... application of SH2 domain binding peptides, which results in blocking of the binding of the SH2 domain to the substrate and thereby preventing interaction of the substrate with the kinase domain. For ... Y527FD1)250N-terminal, SH3, SH2, kinase domain and C-terminusDeletion of N-terminal, SH3 and SH2 domain of kinase- dead n -Src B. R. Groveman et al. A novel function of Src SH2 and SH3 domains FEBS Journal ... cellular Src (c -Src) and neuronal Src (n -Src) , are found in neurons. n -Src contains a sixamino acid insertion in the SH3 domain, and is onlyexpressed in neurons [3]. The SH3 and SH2 domains in Src...

Tài liệu Báo cáo khoa học: FGF-2, IL-1b and TGF-b regulate ﬁbroblast expression of S100A8 doc

... embryo-genesis and synthesis of in ammatory cytokines [43,52] and is essential for S100A8 induction in macrophages[27]. Inhibitors of the MEK and p38 pathways bothpartially abrogated FGF-2 ⁄ heparin- and ... (1 : 200, in saponin–BSA) for 1 h in the dark at 25 °C, rinsed in NaCl ⁄ Pi(3 · 5 min) and nucleistained with DAPI (0.3 nm in NaCl ⁄ Pi, 10 min in the dark).Slides were mounted in Vectashield ... the S100 family of Ca2+-binding proteins and are now accepted as markers of in ammation. They areexpressed by keratinocytes and in ammatory cells in human ⁄ murine wounds and by appropriately...

Tài liệu Báo cáo khoa học: "A Multimodal Interface for Access to Content in the Home" pdf

... understanding of system capa-bilities. Of the remaining 66% of inputs (2166) which were within the functionality of the system, 68% were in grammar. On the within functionality portion of the ... and pen input, includ-ing both pointing and handwriting (Figure 1). Our application task also differs, focusing on search and browsing of a large database of movies-on-demand and supporting ... completing the same two sets of scenarios and then freely interacting with each system. For counterbalancing purposes, half of the subjects used the tablet and then the re-mote control and the other...

Tài liệu Báo cáo khoa học: Roles of AP-2 transcription factors in the regulation of cartilage and skeletal development doc

... apoptosis [73]. The authors of this studyascribe the regulation of outgrowth of limb buds and patterning of the digits to the chicken AP-2. The role of AP-2a was further studied in zebraﬁsh.It ... role of AP-2a and AP-2e in chondrogenesis. Overview of the differen-tiation stages during chondrogenesis and the involvement of transcription factors(henatoxylin and eosin-stained section of ... crucial roles in pathologicalprocesses such as tumorigenesis and genetic diseases[47].Most analyses of the regulation of AP-2 and the interactions of the transcription factor with bindingpartners,...

Tài liệu Báo cáo khoa học: Roles of the human Rad51 L1 and L2 loops in DNA binding doc

... permonomer of poly(dT), showing that the changes weremainly due to the binding of the ﬁrst DNA, while the binding of the second DNA had less in uence(Fig. 5A). To ensure that the estimation of the ... located, and are not found at the ATP-binding siteor the subunit–subunit interface of the Rad51 ﬁlament.Therefore, the Rad51 L1 and L2 loops may also beinvolved in DNA binding. In the present ... ssDNA binding, but notdsDNA binding [22], in contrast to the case of the ABFig. 5. Fluorescence changes of tryptophan probes inserted in the L1 and L2 loops of Rad51 upon poly(dT) binding. The...

Tài liệu Báo cáo khoa học: Roles of heat shock factors in gametogenesis and development pptx

... section and scheme of the coronal section at E14.5 in (F). (A) At E11.5, HSF2 is expressed in forebrain, midbrain and in hindbrain except for the midbrain, hindbrain midline. Surprisingly, the HSF2 ... neurogenesis and neuronal migration in different parts of the mousebrain. HSF1 and HSF2 might interact in the develop-ing mouse brain in a stage- and tissue-speciﬁc region. The levels of HSF1 at ... division, as a result of the defective migration of the spindle during cytoki-nesis. In this case, the depletion of Hsp90a in the absence of HSF1 affects the mitogen-activated protein kinase pathway....

Tài liệu Báo cáo khoa học: Local stability identiﬁcation and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf

... role in maintainingSNase tertiary structure.Our CD and DSC data show that the W140 in SNase is the amino acid responsible for the stability of the whole protein. However, in comparison with the wild-type ... located in the C-terminal loop of SNase playsan important role in protein stability and conforma-tional integrity. During protein unfolding ⁄ refolding, the addition of 2.5 protons (both E75 and ... in maintaining the native structure of SNase. In contrast, W140A and W140O revealed no distinctmaxima in their DSC proﬁles. This is very differentfrom the wild-type protein and K133A and E142O,which...

Tài liệu Báo cáo khoa học: Complete subunit sequences, structure and evolution of the 6 · 6-mer hemocyanin from the common house centipede, Scutigera coleoptrata pptx

... raised against the hemocyanin fraction showstaining of the two 75 kDa bands in Western blot, but donot recognize any other proteins of the hemolymph.Although we expected that each of these bands ... observed in SDS/PAGE and in 2D PAGE (Figs 1 and 2; Table 1). The N-terminal amino acid sequences (see above) allow the assignment of the HcA clone to the major sequence of the upper hemocyanin band in ... bands with apparent molecularmasses in the range of 75 kDa, but no other contaminatingprotein detectable by Coomassie staining, suggesting that the preparation results in >95% pure protein...

Tài liệu Báo cáo khoa học: "An Information-Theory-Based Feature Type Analysis for the Modelling of Statistical Parsing" docx

... headword of the node. In the following, the units of PIQ are bits. ConclusionAmong the feature types in the same structuralposition of the parsing tree, the predictiveinformation quantity of lexical ... sequencecontaining the leaf nodes of the partial parsingtree rooted by the current node, representing the final objectives to be derived from the currentnode.4.2 The corpus used in the experiment The ... brother)0.2505(Y= the first left brother of the parent)0.0949(Y= the second left brother)SD=2 0.6483(Y= the grandpa)0.1066(Y= the second right brother)0.1068(Y= the first rightbrother of the parent)...

Báo cáo khoa học: Roles of conserved arginines in ATP-binding domains of AAA+ chaperone ClpB from Thermus thermophilus pptx

... arginines called the argininefingers. The roles of arginine fingers have beenexplored in several AAA+ proteins [3–8]. The argininefinger is typically located in the subunit interface, and interacts ... conservedWalkerA and WalkerB motifs, and two arginines (AAA-1) or one arginine(AAA-2). Here, we investigated the roles of these arginines (Arg322,Arg323, and Arg747) of ClpB from Thermus thermophilus in the ... arginine(P2) in AAA-1 was also unclear. Here, we investigated in detail the roles of these three arginines in TClpB.By combining an arginine mutation with the WalkerAmutation in the other...

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