Báo cáo khoa học: Deﬁciency in apolipoprotein E has a protective effect on diet-induced nonalcoholic fatty liver disease in mice pot

... compilation ª 2011 FEBS 3129 Deficiency in apolipoprotein E has a protective effect on diet-induced nonalcoholic fatty liver disease in mice Eleni A. Karavia1, Dionysios J. Papachristou2, Ioanna ... developsAbbreviationsapoE, apolipoprotein E; apoE) ⁄ ), apoE deficient; apoE3knock -in mice, mice containing a targeted replacement of the mouse apoE gene for thehuman apoE3 gene; FFA, free fatty acid; ... weeks, and histological andbiochemical analyses were performed. We found that deficiency in apoE has a protective effect on diet-induced hepatic triglyceride accumulation, and theapoE-mediated...

Báo cáo khoa học: Deﬁnition of the residues required for the interaction between glycine-extended gastrin and transferrin in vitro pptx

... gastrin–ApoTf complex, EDTA wasinjected into the BIAcore to chelate any available iron.As soon as the EDTA was injected, the associationbetween gastrins and ApoTf was disrupted, indicatingthat ... interaction in iron homeostasis. In thepresent work, the interaction between gastrins and transferrin has beencharacterized by surface plasmon resonance and covalent crosslinking.First, an interaction ... interaction between iron-free apo-transferrin and Gamide or Gglywas observed. The fact that no interaction was observed in the presence ofthe chelator EDTA suggested that the gastrin–ferric ion...

Báo cáo khoa học: De novo synthesis, uptake and proteolytic processing of lipocalin-type prostaglandin D synthase, b-trace, in the kidneys pptx

... be a useful diagnostic marker for renalinjury associated with diabetes or hypertension, because the urinary andplasma concentrations are increased in patients with these diseases. How-ever, ... size markers are shown on the left. (B) Schematic representation of GST-fusionproteins containing a series of amino-terminus-truncated human L-PGDS. The amino-terminal amino acid residue of each ... Each ampliﬁed fragment was cloned downstream ofGST in the pGEX-2T vector. Expression, puriﬁcation andanalyses of recombinant proteins were carried out asdescribed above.N-terminal amino acid...

Báo cáo khoa học: The mystery of nonclassical protein secretion A current view on cargo proteins and potential export routes pdf

... functions also serve as paracrine signallingmolecules upon unconventional release into the extracellularspace? The answers to these questions are of exceptionalinterest as the cargo proteins secreted ... ismediated in a temperature-sensitive manner by proteina-ceous machineries that are insensitive to brefeldin A. Leishmania HASPBAnother quite remarkable example of nonclassical proteinexport ... proteins arepackaged into exosomes (see above). An engineered versionof galectin-3 containing an N-terminal acylation motifderived from a protein tyrosine kinase (p56lck) has beenshowntobesecretedmoreefﬁcientlythanwild-typegalectin-3...

Báo cáo khoa học: Saccharomyces cerevisiae a1,6-mannosyltransferase has a catalytic potential to transfer a second mannose molecule ppt

... A ) indicating the ﬁrst mannose additionwere detected as intermediates (Fig. 6A) . To examinethe intermediate structures, the fraction containingproducts A and A was collected and treated with ... (5¢-CAAGAGGTGGTATTTACTCAGCTATGGATACTATGCTTTTGAA-3¢) and D18 8A- RV (5¢-TTCAAAAGCATAGTATCCATAGCTGAGTAAATACCACCTCTTG-3¢),and the pPICZaA-ScOCH1 as a template. The bothD18 8A mutant and wild-type proteins were expressed asmentioned above. ... that peak 3 represents py-ridylaminated oligosaccharide in which one moleculeof hexose has been added to Man10GlcNAc2-PA. Thisadditional hexose is probably a mannose residuebecause the...

Báo cáo khoa học: The Thermoplasma acidophilum Lon protease has a Ser-Lys dyad active site pot

... experimentally [11].Notably, the archaeon Methanosarcina mazei has t wo longenes, an ar chaeal-type lon gene containing two transmem-brane helices and a bacterial-type lon gene including anN-terminal ... ATPase domain.Noncanonical Lon proteases are also found in bacteria andviruses (Fig. 1B,C). A separate study failed to detect theATP-binding region in the archaeal Lon proteases in sequence ... aresoluble, the archaeal c ounterpart is membrane-attached[13]. Bacterial LonB proteases, like archaeal Lon, lack theLAN domain p resent in bacterial L onA pro teases, but likebacterial LonA, also...

Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): evaluation of the biological function of growth-inhibitory factor in the injured and neurodegenerative brain pdf

... For instance, in thelater stages of neurodegenerative diseases the neuronaldamage may interfere with the neuroglial interactions,lowering GIF production and secretion in reactiveastrocytes. ... GIFAD is a neurodegenerative disease that leads to severedementia and ultimately death. The pathologicalhallmarks of the disease are intracellular neuroﬁbril-lary tangles, dystrophic neurites ... neuronal exhaustion and eventu-ally cell death, partly explaining the vast neuronal loss in the AD brain. Further investigation of the increasedneurotrophic activity of the AD brain revealed...

Tài liệu BÁO CÁO KHOA HỌC ĐỀ TÀI: "NGHIÊN CỨU ĐỀ XUẤT BIỆN PHÁP PHÒNG NGỪA VÀ PHƯƠNG ÁN ỨNG PHÓ TRÀN DẦU MỨC I TẠI THÀNH PHỐ ĐÀ NẴNG ppt

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Tài liệu Báo cáo khoa học: Time-dependent regulation analysis dissects shifts between metabolic and gene-expression regulation during nitrogen starvation in baker’s yeast doc

... Samples were taken tomeasure the whole-cell protein concentration, fermentativecapacity, mRNA levels and the capacities of the glycolyticand fermentative enzymes. After all samples had beentaken, ... experiments, the steady-state ﬂuxeswere estimated. Averages and SD were calculated separatelyfor the numerator and the denominator of Eqn (5). Based on the SD of the numerator and the denominator ... Regula-tion coefﬁcients were calculated according to the integrative time-dependent regulation analysis (see Introduction). The error bars representSEM of three independent experiments carried out on...

Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc

... whether the observed IL-1bmRNA changes are due to a decrease in transcriptionor an increased degradation rate, we performed experi-ments with actinomycin D. HepG2 cells stablyoverexpressing ... the MCPIP gene wasobserved in leukocytes, with intermediate levels in heart and placenta, and the lowest levels in spleen, kid-ney, liver and lung. The transcript was not detectable in brain, ... were performed to deter-mine the level of exogenous IL-1b mRNA.Reporter gene assayTransient transfection experiments were performed usingLipofectamine 2000 reagent (Invitrogen) in a 12-well...

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