Báo cáo khoa học: Binding afﬁnities and interactions among different heat shock element types and heat shock factors in rice (Oryza sativa L ) ppt

Báo cáo khoa học: Binding afﬁnities and interactions among different heat shock element types and heat shock factors in rice (Oryza sativa L.) ppt

... Journal 278 (201 1) 3076–3085 ª 2011 The Authors Journal compilation ª 2011 FEBS 3085 Binding affinities and interactions among different heat shock element types and heat shock factors in rice (Oryza ... class A-HSFs, using anovel BiFc fragment, and identification of novel classB-HSF interacting proteins. Eur J Cell Biol 89,126–132.D. Mittal et al. Heat shock element types and heat shock factors ... Technical support issues arisingfrom supporting information (other than missing files)should be addressed to the authors.D. Mittal et al. Heat shock element types and heat shock factors in rice FEBS...

Báo cáo khoa học: Binding afﬁnity of nonsteroidal ecdysone agonists against the ecdysone receptor complex determines the strength of their molting hormonal activity pot

... Japan) in low-salt buffer(20 mMHepes, 20 mMNaCl, 20% glycerol, 1 mMEDTA,1mM2-mercaptoethanol, pH 7.9, containing 1 lgÆmL )1 aprotinin, 1 lgÆmL )1 pepstatin and 1 lgÆmL )1 leupeptin)with3H-labeled ... (PerkinElmer LifeSciences, Wellesley, MA, USA) using a liquid scintillationcounter LSC-1000 (Aloka Co., Ltd, Tokyo, Japan).Ligand binding to the cell-free preparation fromC. suppressalisintegument Rice ... superfamily, whichis generally composed of six distinct regions (A–F), and thepredicted amino acid sequences of C (DNA binding) and E(ligand binding) regions are conserved among insects [11].Little...

Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx

... Sequestering of the ﬁbrilsinto intracellular inclusions or extracellular plaquesmight actually be beneﬁcial, as it is now believed thatsoluble oligomers are the cause of the initial insultrather ... protein. Typically an initial injection of 2 lL copperchelate solution was followed by a further 29 injections of4 lL of Cu(II) into the protein in the sample cell stirredat 300 r.p.m. Injections ... (II) and Cu2+(II) binding to His, GlyGlyHis, HisGlyHis, and bovineserum albumin: a critical evaluation. Inorg Chem 39,3057–3064.55 Dawson RMC, Elliot DC, Elliot DH & Jones KM(198 6) Data...

Tài liệu Báo cáo khoa học: High-afﬁnity ligand binding by wild-type/mutant heteromeric complexes of the mannose pptx

... mutant (no binding activity) and wild-type (100% binding activity) mini-receptors in themixture would follow the line displayed in the bargraph, suggesting that the wild-type binding site on ... Theseresults can be explained either by a stericallyimprobable intramolecular binding mechanism or by binding of a multivalent ligand forcing receptors torealign within the immunoprecipitated complexes,thus ... (198 6) Analysis of serum insulin-like growthfactor binding proteins using western blotting: use of themethod for titration of the binding proteins and competi-tive binding studies. Anal Biochem...

Tài liệu Báo cáo khoa học: Binding of N- and C-terminal anti-prion protein antibodies generates distinct phenotypes of cellular prion proteins (PrPC) obtained from human, sheep, cattle and mouse doc

... full-length PrP was ana-lyzed after deglycosylation. While N-terminal binding antibodies as SAF34 detected only full-length PrPC,C-terminal binding antibodies recognized two bandscomprising ... post-translational modiﬁcation involving removal of anN-terminal signal peptide and C-terminal residues in the polypeptide chain and attachment of a glycosyl-phosphatidylinositol group for cytoplasmic ... determinedusing serial dilutions of samples (Fig. 2). Linearityconsisting of continuous signal increase and of repro-ducible glycoprotein patterns was determined in therange between 4 and 10 lL of...

Báo cáo khoa học: N-Glycosylation is important for the correct intracellular localization of HFE and its ability to decrease cell surface transferrin binding pptx

... onHFE intracellular trafﬁcking, localization and func-tion. Initially this was done in the context of inhibitingcellular glycan production, with tunicamycin treatmentaltering HFE localization ... glycosylated proteins.As might be expected from the ER-localized and potentially misfolding triple mutant, we observed littleor no regulation of transferrin binding in cells express-ing glycosylation-deﬁcient ... transferrin, and that little or no reduction in transferrin binding was observed in cells expressing theHFE-C282Y mutant (Fig. 7A).ABFig. 5. Intracellular localization of HFEdouble N-glycosylation...

Báo cáo khoa học: Binding of the volatile general anesthetics halothane and isoﬂurane to a mammalian b-barrel protein doc

... Kdvalues of80 ± 10 lm and 100 ± 10 lm, respectively. Halothane and isoflurane binding resulted in an overall stabilization of the folded conformation ofthe protein by )0 .9 ± 0.1 kcalÆmol )1 . In ... other ligands [23] shown crystallographically tooccupy the internal cavity of the protein [24]. Briefly, por-cine odorant binding protein samples (1 lm), containing afixed amount of AMA (1 lm), ... unit (eu) is calÆmol )1 Æ K )1 .Anesthetic Kd(lM) DG° (kcalÆmol )1 ) DH° (kcalÆmol )1 ) DS° (eu)Halothane 80 ± 10 )5 .5 ± 0.1 )1 .4 ± 0.1 14.0Isoflurane 100 ± 10 )5 .4 ± 0.1 )2 .4 ± 0.1 10.3Fig....

Báo cáo khoa học: Frontal afﬁnity chromatography analysis of constructs of DC-SIGN, DC-SIGNR and LSECtin extend evidence for afﬁnity to agalactosylated N-glycans potx

... N-acetylglucosamine; GnT, N-acetylglucosaminyltransferase; ICAM, intracellular adhesion molecule; LPS, lipopolysaccharide;LSECtin, liver and lymph node sinusoidal endothelial cell C-type lectin; ... proteins toLec8 cells was abolished.We then performed inhibition tests using a GlcNAc- binding lectin from Psathyrella velutina (PVL). WhenPVL (1 mgÆmL )1 ) was pre-incubated with Lec8 cells, binding ... IgG, and incu-bated with the Lec1, Lec8 and CHO cell lines(Fig. 5A). DC-SIGN–Fc bound strongly to Lec8 cellsas well as to Lec1 cells (Fig. S 4), but did not bind toparental CHO cells. Similarly,...

Báo cáo khoa học: Binding of berberine to human telomeric quadruplex – spectroscopic, calorimetric and molecular modeling studies pot

... described in the text. Values determined from microcalorimetric data.Temperature (°C) DH (kcalÆmol )1 ) Kb· 10 )6 (M )1 ) n DG (kcalÆmol )1 ) TDS (kcalÆmol )1 ) DCp(calÆmol )1 ÆK )1 ) 10 )0 .2 ... change, using DG = DH)TDS.Kb(·106M )1 ) DG (kcalÆmol )1 ) DH (kcalÆmol )1 ) TDS (kcalÆmol )1 ) 1.2 ± 0.1 )8 .2 ± 0.8 )1 .7 ± 0.2 6.5 ± 0.7A. Arora et al. Telomeric quadruplex–berberine interactionFEBS ... still be used to accurately and directlymeasure the binding enthalpy and stoichiometry forthis interaction. ITC remains an invaluable techniquefor determining binding enthalpies, even in cases...

Báo cáo khoa học: Binding and activation of nitric oxide synthase isozymes by calmodulin EF hand pairs potx

... fertilization, cellproliferation, cell defense, and cell death [1]. Calmodu-lin (CaM), a ubiquitous 17-kDa cytosolic protein, isa major cellular Ca2+sensor which rapidly regulatesintracellular processes ... domains bind NADPH,FAD, and FMN, and the oxygenase domain con-tains binding sites for heme, tetrahydrobiopterin(H4B), and the substrates l- arginine and molecularoxygen [7]. A CaM -binding ... co-ordinatedactivation of over 50 intracellular proteins [2]. Becauseof the manifold and diverse roles of CaM in intracellu-lar signaling, there is signiﬁcant interest in betterunderstanding...

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