... underphysiological conditions, the majority of Cl-ACL binds toonly one molecule of citrate. In the presence of sufficientlevels of ATP, the low Ks1value would contribute to the efficient conversion of citrate ... studies, the most striking feature of Cl-ACLwas the strong negative cooperativity observed towardscitrate binding. As Ks1<<Ks2<<Ks3, binding of the firstand second molecules of ... METHODSPurification of the recombinantCl-ACLConstruction of the expression vector pETACL harboring the aclBA genes from C. limicola strain M1, and the expression procedure of the genes in E. coli...