... forward:5¢-CCGAATTCGAAGAAATCACACTTGAAAGG-3¢, andreverse: 5¢GGATCCCCATCATTCATATACATACTTGTGGGTT-3¢; PDZ3, forward: 5¢CCGAATTCCTTGGAGATGATGAAATTACAAGGG-3¢, and reverse: 5¢GGATCCATTCTTCAGGTCGATATTGTGCAAC-3¢. ... hDlg interacting partners in hVSMCs. Accordingly, we used a vector encoding the hDlg PDZ1 and PDZ2 domains as bait in a yeasttwo-hybrid screening assay of a human aorta cDNAlibrary. Interestingly, ... exhibited a diffuse staining with anoccasional patchy appearance and both types of label-ing partially colocalized in these patches, suggesting the presence of aggregates (Fig. 5A) . In addition, the localization...