Tài liệu Báo cáo Y học: Toxicity of substrate-bound amyloid peptides on vascular smooth muscle cells is enhanced by homocysteine docx
Tài liệu Báo cáo Y học: Toxicity of substrate-bound amyloid peptides on vascular smooth muscle cells is enhanced by homocysteine docx
... inhibition of adhesion and subsequent toxicity to the cells by Ab may beimportant in cerebrovascular pathogenesis of amyloid angiopathy. The greater toxicity of the longer Ab1–42species is consistent ... Toxicity of substrate-bound amyloid peptides on vascular smooth muscle cells is enhanced by homocysteine Su San Mok1,2, Bradley J. Turner1,2, Konrad Beyreuther3, Colin ... form of CAA consists of proteinaceous deposits of amyloid- b protein (Ab) that occur adjacent to vascular smooth muscle cells (VSMC). Ab consists of 39–43 aminoacids and is proteolytically derived...
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Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf
... selectivity andspecificity in the mononuclear SODs is not governed by one or even two residues, but is most likely accomplished by the concerted effects of a combination of key residues.Further analysis ... differen-tial effects of hydrogen peroxide on the FeSOD mutantsmay be explained by an alteration of reactivity induced by the electronic configuration of active site residues, in thiscase glutamine ... manganese.Selectivity of the proteins for their metal cofactor has beendemonstrated in vivo [3] and although apoenzymes of eachtype of SOD may be reconstituted by the addition of metals,the resulting enzyme is...
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Tài liệu Báo cáo khoa học: Analysis of proteins and peptides on a chromatographic timescale by electron-transfer dissociation MS ppt
... Journal compilation ª 2007 FEBS 6271dimethylation of Arg, monomethylation, dimethylationand trimethylation of Lys, acetylation and ubiquitina-tion of Lys, and phosphorylation of Ser and Thr ... 1. Fragmentation scheme for production of ions of type c¢ andz¢Æ by reaction of a low-energy electron with a multiply protonatedpeptide.ETD-MS analysis of peptides and proteins N. D. Udeshi ... LY, Coon JJ, Syka JE,Bai DL, Shabanowitz J, Burke DJ, Troyanskaya OG &Hunt DF (2007) Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electrontransfer dissociation...
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Tài liệu Báo cáo khoa học: Transcription of individual tRNA1Gly genes from within a multigene family is regulated by transcription factor TFIIIB pdf
... inhibition of tRNAGly1-1 by the compet-ing tRNAGly1-6,7 (lane 3), was rescued very efficiently by increasing concentrations of TFIIIB (lanes 5 and 6)but not by pol III (lane 4). Transcription ... inthe sequestration of TFIIIB presumably by binding tothe TBP component of TFIIIB.This inference was further confirmed by immuno-depletion of TFIIIB using a polyclonal antibody direc-ted against ... Sequestration of TFIIIB by interactions with the TATA sequences in the flanking regions of tRNAGly1genes. (A) Competition by DNA fragments containing TATATAA sequences. Transcription of tRNAGly1-1...
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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
... substratespecificity of the enzyme. The degradation of the polymerand subsequent oligosaccharide formation was analyzed by HPAEC and MALDI-TOF MS. HPAEC analysis demon-strated that already after 15 ... andmethods.Hydrolysis of arabinogalactansThe sugar composition of the arabinogalactans frompotato, onion and soy was determined as described [21].Onion arabinogalactan consists of 99%D-galactose ... resulting in a 30% reduction of the galactosylcontent of the pectin fraction of the cell walls [17].In this paper we describe the cloning, characterizationand expression analysis of the A. niger galA...
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Tài liệu Báo cáo Y học: Inactivation of the 2-oxo acid dehydrogenase complexes upon generation of intrinsic radical species pptx
... complex-catalyzed succinyl-CoA hydro-lysis [72] accounts for the inactivation by dihydrolipoamidein the presence of succinyl-CoA (Table 2).DISCUSSIONGeneration of radical species during catalysis by ... backward reaction of NADH oxidation[22]. Under physiological conditions, this reaction is unlikelyto contribute to the mitochondrial metabolism significantly:NADH is a strong inhibitor of E3 and ... (0.3M)andcatalase(26UÆmL)1).Distribution of the EPR signal between the protein andnonprotein fractions was investigated after precipitation of 2-oxo acid dehydrogenase complex by addition of 0.2 vol. of 35% polyethylenglycol...
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Tài liệu Báo cáo Y học: Characterization of an omega-class glutathione S-transferase from Schistosoma mansoni with glutaredoxin-like dehydroascorbate reductase and thiol transferase activities pptx
... Purificationyield approximately 500 lg of protein per milliliter of S-hexyl glutathione agarose. In all cases, protein purity wasdetermined by SDS/PAGE and protein concentration wasmeasured by bicinchoninic ... forSmGSTO and the strong inhibitory effect of S-hexylglutathione on the enzyme activity. The preference of SmGSTO for more hydrophobic alkyl-bound S-hexylglutathione rather than glutathione could reflect ... members of the recently discovered omega class of GSTs [10], and wasFig. 4. Expression and purification of His-tagged SmGSTO and native SmGSTO. (A) SDS/PAGE analysis of His-tagged SmGST purified on nickel-agarose....
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Tài liệu Báo cáo Y học: Oxidation of phenols by laccase and laccase-mediator systems doc
... Poliporus pinsitus was kindly donated by Novo Nordisk Biotech and purified by ion-exchangechromatography on Q-Sepharose by elution with phosphatebuffer [5,20], and an activity of 10 000 UÆmL)1determinedspectrophotometrically ... determination of laccase activityThe activity of laccase was determined by following the rate of oxidation of 2,2¢-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) to ABTS radical cation (ABTS•+), ... bedetected by LC-MS, even though the formation of smallamounts of olefinic products is indicated by 1H-NMRspectra also in this case (Fig. 4). There is no evidence of oxidation of the internal methylenes....
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Tài liệu Báo cáo Y học: Characterization of a cloned subtilisin-like serine proteinase from a psychrotrophic Vibrio species doc
... proteinases of this comparative study, we alsodetermined the denaturation curves of the enzymes inhibited by phenylmethanesulfonyl fluoride, as a function of denat-urant concentration. Several ... loops of the C- and N-termini of the protein, calcium is coordinated by the carboxylate oxygens of Asp260 and thebackbone oxygen of Thr16, in addition to 4–5 watermolecules [57,58]. Comparison of ... followed by analysis by SDS/PAGE on 8–25% gels.Table 3. Comparison of thermal stability (T50%) and Michaelis–Mentenparameters (Kmand kcat) against succinyl-AAPF-p-nitroanilide of wild-type...
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Tài liệu Báo cáo Y học: Inhibition of the MEK/ERK signaling pathway by the novel antimetastatic agent NAMI-A down regulates c-myc gene expression and endothelial cell proliferation ppt
... Ventura1,21Department of Biomedical Sciences, Division of Biochemistry, Laboratory of Cardiovascular Research,2Division of Cell Biology,National Institute of Biostructures and Biosystems, and3Department of ... activation is mediated, at least in part, by G-protein coupled receptors (GPCRs), stimulation of membrane phospholipid hydrolysis, and activation of thediacylglycerol sensitive isoforms of protein ... theactivation of ERK by GPCRs closely parallels thatemployed by RTK [33]. On the whole, these observationssuggest that the inhibitory effect elicited by NAMI-A on ERK signaling might have occurred by...
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