... target exon, respect-ively. Given the small size of introns 4 and 10 , primers C45U/C45Land C1 011 U/C1 011 L were designed to amplify t ogether e xons 4 and 5and exons 10 and 11 , respectively. Sequencing ... the same buffer by using 1 mUenzyme/mg casein at 37 °C for 18 h; these c onditions havebeen previously shown to p roduce complete d ephosphory-lation of the sample [ 20]. Reactions were stopped ... the routine determination of the v arious non-casein proteins of milk. 15 th Int. D airy Congr. London 3, 16 31 16 37. 18 . Chianese, L., Mauriello, R., Intorcia, N ., Moio, L. & Addeo, F. (19 92) New as2-casein...