... internal control for RT-PCR. PCR condi-tions were as follows: one cycle of 94 °C for 3 min, 32cycles of 94 °C for 30 s, 54 °C for 30 s and 72 °C for 30 s,followed by one cycle of 72 °C for 5 min. ... EDTA,pH 8.0) and stored at )20 °C before use.Cloning and sequencingInitially, PCR was performed using the cDNA preparedabove, with primers zfstat6-F1, zfstat6-R1, zfstat6-F2 and zfstat6-R2 for stat6,zftbet-F1 ... zfstat6-R1, zfstat6-F2 and zfstat6-R2 for stat6,zftbet-F1 and zftbet-R1 for t-bet, and zffoxp3-F1, zffoxp3-R1, zffoxp3-F2 and zffoxp3-R2 for foxp3. These primers amplified part of the initial predictedsequence...