... characterization of the function of each com-ponent in AtdA, or other closely related aniline dioxy-genases, such as that from Pseudomonas putida UCC22(pTDN1) [14]. The lack of characterization of the structural ... to the enzyme. This may indicate that the steric limitation of the enzyme’s binding pocket takes place in the areabetween the ortho and para positions of the aromaticsubstrate. On the basis of ... extension PCR was used to join the threefragments together. The overlap extension PCR reactionmix consisted of 85 ng of atdA1A2,50ngofatdA3,60ng of atdA4A5,2lLof10· Pfu buffer, 2 lLof10· dNTP(mixture...