... in hydration and the conformationalchanges involved in protein–ligand interactions [16,17], and thus may provide further information on the molecularmechanism of fosfomycin and UDPNAG binding ... charged amino acid sidechains, R397 and R120, are also involved in fosfomycinbinding, and in view of the multitude of interactions, it wasassumed that deletion of a single interaction would not ... chain of K22 participates in fosfomycin binding, thus providing a rationale for the loss offosfomycin binding to the K22V and K22E mutant proteins.However, two other positively charged amino...