Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx
Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx
... structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain Yoko Kobayashi, Toshiaki Nakajima and Ituro InoueDivision of Genetic Diagnosis, Institute of Medical ... orientation, the catalytic site in the N-terminal domain of one subunit and the substrate recognition site in the C-terminal domain of the other face each other, thereby bringing together the substrate-binding ... Graphicsworkstation and was based on the structures of the human and porcine pancreatic lipases for the open form and on the horse pancreatic lipase for the closed form [16,18,19,30].For homology modeling, the...
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Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc
... concentration of 3.4 lm.Thermophilicity and thermostabilityThermophilicity was evaluated in the temperature range40–90 °C by measuring the polymerase activity of the Rep245 under standard conditions ... Interestingly, thisproperty is shared by the X family of human DNApolymerases, which includes the TdT enzymes and twoadditional members, Pol k [29] and Pol l [30]. The latter two enzymes are functionally ... activesite of the X family of polymerases [23]. Indeed, unlike the pRN1 prim–pol protein whose motif is DXE D, the Rep245 protein, the X family of DNA polymerases and the TdT enzymes have the DXD...
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Tài liệu Báo cáo Y học: Convulxin induces platelet shape change through myosin light chain kinase and Rho kinase ppt
... by Rho kinase and the other by the Ca2+-dependent activation of MLCkinase. These two pathways could be activated independ-ently of each other, depending on the type and concen-tration of ... inhibition of MLC phosphorylation, as demon-strated by the experiments with BAPTA, which onlydiminished MLC phosphorylation by about 50%. The enhanced capability of cAMP of inhibiting MLC phos-phorylation ... 19.8% and 15% reduction, respectively(Fig. 3A). The identity of the 20 kDa band detected was confirmedby immunoblot analysis by means of an anti-MLC mono-clonal Ig (data not shown).Inhibition of...
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Tài liệu Báo cáo Y học: Ornithine decarboxylase-antizyme is rapidly degraded through a mechanism that requires functional ubiquitin-dependent proteolytic activity pot
... prepared and analysed asdescribed in A. The molecular weight and position of the two forms of antizyme is indicated on the right. *NS indicates the position of nonspecificproteins recognized by the ... antizyme was rapidly degraded inboth the presence and the absence of coexpressed ODC(Figs 2C and D). Similarly, we have noted that antizymethat was induced by the addition of spermidine to the growth ... previous study [22]. The interpretation in that study wasFig. 4. Degradation of antizyme depends on the integrity of the ubiqui-tination machinery. Wild-type (A31N) and ts20 cells containing ther-mosensitive...
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Tài liệu Báo cáo Y học: Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling ppt
... of the loop indicated by the letter a.Fig. 5. Scheme of the binuclear manganese cluster and the localization of the side-chains of Asp153 and Asn153 in the modelled structures of wild-type and ... indicating that the environ-ment of tryptophan residues is essentially conserved in the mutant enzyme. On the other hand, the absence of majordifferences in the CD spectra of the wild-type and D153Nagmatinases ... caldovelox and rat liver arginases alsoFig. 2. Effect of added Mn2+ and EDTA on the catalytic activity of wild-type and D153N agmatinase. The enzymes were assayed before(Control) and after dialysis...
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Tài liệu Báo cáo khoa học: Molecular basis of glyphosate resistance – different approaches through protein engineering doc
... some of the key findings that have led to our current under-standing of the molecular mode of action of glypho-sate and the molecular basis for glyphosate resistance. Structure and function of ... (blue) structures shows the dif-ferent conformations of the main chain of the a2–a3 loop, seearrows [49]. For the sake of clarity, only the FAD and the ligandbelonging to the wild-type GO structure ... EPSPSEPSPS catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate 3-phosphate (S3P) to produce 5-enolpyruvylshikimate 3-phosphate (EPSP) and inorganic...
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Tài liệu Báo cáo khoa học: Molecular defect of isovaleryl-CoA dehydrogenase in the skunk mutant of silkworm, Bombyx mori ppt
... conservedmotif of Gly–Gly–X–Gly. The second glycine of thismotif (Gly374) is known to make a hydrogen bondwith the pyrophosphate moiety of the FAD of the nextmonomer in the tetramer [16]. However, the ... 3-methylcrotonyl-CoA. IVD isa member of the acyl-CoA dehydrogenase (ACAD)family of enzymes, all of which share significantsequences and employ a similar enzyme mechanism for the a,b-dehydrogenation of ... is the first report of IVD deficiency in animals aside fromhumans.IVD is a member of the ACAD family of enzymes,all of which employ a similar enzyme mechanism fora,b-dehydrogenation of acyl-CoA...
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Tài liệu Báo cáo khoa học: Molecular characterization of Arabidopsis thaliana PUF proteins – binding specificity and target candidates doc
... ladders of helices running through the domain [18,19]. The crystal structure of the human Pumilio ⁄ NRE complex demonstrated that each repeat of the PUF domain recognizes a single nucleotide in the ... cells.Screening of the Arabidopsis RNA libraryYeast strain YBZ-1 [40] was co-transformed with APUM-2protein hybrid and the hybrid RNA plasmids. Cells weregrown on yeast complete synthetic medium ... PUF-8,Drosophila and human Pumilio and yeast Puf3 recog-ABCDFig. 6. Analysis of binding affinity between APUM-2 and the FAS-2 3¢ UTR transcripts with nucleotide substitutions upstream and down-stream of the...
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Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulfide isomerase family proteins with nonclassic active center motifs pdf
... stages of development. The amounts of pro-b-conglycinin and proglycinin are considered to be nearly equivalent to the synthesis levels of both b-conglycinin and glycinin,as pro-b-conglycinin and ... folding and quality control of nascent polypeptides[25]. In soybean cotyledon, large amounts of seed stor-age proteins such as glycinin and b-conglycinin aresynthesized and translocated into the ... thioredoxin domains between amino acids65–164 and 403–481, and 68–167 and 406–508,respectively, corresponding to the a -domain and a¢ -domain of PDI [7]. Notably, both GmPDIL-3a and GmPDIL-3b lack the...
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Tài liệu Báo cáo khoa học: Molecular and cellular specificity of post-translational aminoacyl isomerization in the crustacean hyperglycaemic hormone family docx
... 481–485.10 Ollivaux C & Soyez D (2000) Dynamics of biosynthe-sis and release of crustacean hyperglycemic hormoneisoforms in the X-organ-sinus gland complex of the crayfish Orconectes limosus. ... Toullec JY & Soyez D (2002)Enkephalinergic control of the secretory activity of neu-rons producing stereoisomers of crustacean hyperglyce-mic hormone in the eyestalk of the crayfish Orconecteslimosus. ... microscopystudy of the neuronal endings in the sinus gland todetermine the subcellular localization of the differentepimers. Taken together, these results allow a discus-sion of the existence of...
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