... staining solution (10 mg of propidiumiodide, 0.1 mL of Triton-X, 3.7 mg of EDTA in 100 mL of NaCl ⁄ Pi) was added to 0.5 mL of cell suspension. The sam-ples were incubated in the dark in the ... sine function wasapplied in both dimensions of the time domain beforeFourier transformation.Integration of 1D and 2D signals was performed using1D winnmr and 2D winnmr software (Bruker, AG,Darmstadt, ... study[26], the size of each rectangle marking the area used for the volume integration of a peak was determined such thatit just covered the peak (above the noise) but not the neighbouring peaks....