Tài liệu Báo cáo khoa học: Dimer asymmetry and the catalytic cycle of alkaline phosphatase from Escherichia coli doc
Tài liệu Báo cáo khoa học: Dimer asymmetry and the catalytic cycle of alkaline phosphatase from Escherichia coli doc
... Dimer asymmetry and the catalytic cycle of alkaline phosphatase from Escherichia coli Stjepan Orhanovic´ and Maja Pavela-VrancˇicˇDepartment of Chemistry, Faculty of Natural Sciences, ... catalysis of bothsubunits.Model representation of the catalytic cycle for APase from E. coli A model describing the catalytic mechanism of APase from E. coli, based on the results of the kinetic ... to the magnesium binding site M3 [4].Fig. 4. The influence of Mg2+on the kinetic properties of APase from E. coli. The influence of Mg2+on the kinetic properties of APase from E. coli inthepresenceofaZn2+to...
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Tài liệu Báo cáo khoa học: "Deriving an Ambiguous Word’s Part-of-Speech Distribution from Unannotated Text" doc
... that the word pair consisting of the left and right neighbor of a particular token is characteristic of the part of speech at this position, and by clustering the neighbor pairs on the basis ... be sur-rounded by the pair the is”, a verb by the pair “he the , and an adjective by the pair the thing”. For ease of reference, in the remainder of this paper we call these local contexts ... pairs. The idea is now to cluster the neighbor pairs on the basis of the middle words they occur with. This way neighbor pairs typical of the same part of speech are grouped together. For...
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Tài liệu Báo cáo khoa học: Nucleolin/C23 mediates the antiapoptotic effect of heat shock protein 70 during oxidative stress pptx
... the condensation and fragmentation of theirnuclei. The percentage of apoptotic cells was calculated as the number of apoptotic cells divided by the total number of cells counted. A minimum of ... lost.Taken together, our results demonstrate an impor-tant relationship among the regulation of nucleo-lin ⁄ C23, the activation of caspase-3 and the induction of apoptosis under the setting of oxidative ... notclear. In the present investigation, we further studied the effects of nucleolin ⁄ C23 ablation on Hsp70-medi-ated protection from apoptosis and examined the expression and stability of nucleolin...
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Tài liệu Báo cáo khoa học: Marine toxins and the cytoskeleton: okadaic acid and dinophysistoxins pptx
... being the most studied of these. IFsmaintain cell shape and the structural integrity of cellcontents, and provide protection against various types of stress. The mechanism of action of OA as ... metabolism and coor-dination of the cell cycle and gene expression. The crit-ical importance of phosphatases in cell metabolism isunderlined by the fact that they are targets of naturaltoxins such ... most of the reports were focused on the role of phosphatases in the maintenance of cell cyto-skeleton integrity. With this purpose, DSP toxins havebeen widely employed, with OA being one of the...
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Tài liệu Báo cáo khoa học: Marine toxins and the cytoskeleton: a new view of palytoxin toxicity ppt
... palytoxin action against the actincytoskeleton (Fig. 3). At present, in spite of the impor-tance of the unpolymerized actin pool for the mainte-nance of the F-actin system, the cellular responseFig. ... constitute the actin cytoskeleton[13]. For a long time, it was believed that the role of this system was mainly structural, providing the sup-port needed to maintain the shape and organization of the ... Examination of the structure shows that thereis, in fact, a group of different palytoxins, whosemolecular weights vary according to the species from which they are obtained. Several biogenic origins of palytoxins...
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Tài liệu Báo cáo khoa học: Marine toxins and the cytoskeleton: pectenotoxins, unusual macrolides that disrupt actin pptx
... beenisolated from the digestive glands of scallops collectedin Japan, and these are stereoisomers of PTX-1 and PTX-6, respectively. After acid treatment of PTX-4 and PTX-7, two isomers, named PTX-8 and ... supports the idea that lactone ring integrity is essential for the action of PTXs; oxidation on C43 decrease the toxic-ity of the molecule, and oxidation on C34 does notaffect the potency of PTXs.Unusual ... nucle-ation. On the basis of models of the actin filament,PTX binding would disrupt key lateral contactsbetween the PTX-bound actin monomer and the lowerlateral actin monomer within the filament, thereby...
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Tài liệu Báo cáo khoa học: Oxidative stress in the hippocampus after pilocarpineinduced status epilepticus in Wistar rats doc
... standard light ⁄ dark cycle (lights on at07:00 h). The experiments were performed according to the Guide for the Care and Use of Laboratory Animals of the US Department of Health and Human Services, ... measured in the control (n ¼ 9) and pilocarpine (n ¼ 8) groups by the method that uses H2O2togenerate H2O and O2[36]. The activity was measured by the degree of this reaction. The standard ... produced in the brain. H2O2is convertedinto water by catalase and glutathione peroxidase,which involves GSH, a cofactor of this enzyme [5,8].GSH is one of the most important agents of the cellularantioxidant...
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Tài liệu Báo cáo khoa học: Guanidinium chloride- and urea-induced unfolding of FprA, a mycobacterium NADPH-ferredoxin reductase Stabilization of an apo-protein by GdmCl docx
... GdmCl on the secondary structure of the protein. In the far-UVregion, the CD spectra of the FprA show the presence of substantial a-helical conformation [15]. Fig. 1C sum-marizes the effect of increasing ... C-terminalregions of the enzyme, and the NADPH-bindingdomain (residues 109–323) consisting of the centralpart of the polypeptide chain [8]. A small two-strandedb-sheet links the two domains. ... purityavailable.MethodsOverexpression and purification of FprACloning, overexpression and purification of the FprA wascarried out as described earlier [7]. The ESI-MS and SDS ⁄ PAGE of the purified FprA showed that the prepar-ation...
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Tài liệu Báo cáo khoa học: cN-crystallin and the evolution of the bc-crystallin superfamily in vertebrates pdf
... man [8,9]. In the verteb-rate lens, the b and c crystallins together account for the majority of the soluble proteins (the other majorfamily being the a-crystallins, members of the smallheat-shock ... to others, but is essen-tially an outlier from both b and c families.Overall, these data show a broader and more com-plex view of the evolution of the bc superfamily in the vertebrate eye and ... Notre Dame, IN, USAMuch of the complexity and diversity of life arises from the multiplication and evolution of gene famil-ies, increasing the functional repertoire of the gen-ome. By gene duplication,...
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Tài liệu Báo cáo khoa học: Comparative studies on the functional roles of N- and C-terminal regions of molluskan and vertebrate troponin-I pdf
... ATnI130)252, and ATnI232)292did not migrate into the gels, while free ATnI-52K and ATnI1)128exhibited a band near the origin and at the middle of the gel, respectively. The bands corresponding to the ... understand the biological significance of the differ-ence in TnI–TnC interactions, we compared the ability of TnC to neutralize the inhibitory effects of the C-ter-minal fragments in the presence and ... that the activa-ting effects of the N-terminal region of TnT are exer-ted in the presence of Ca2+by the TnI N-terminalregion (from the structural TnC-binding site to the TnT-binding site) and...
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Từ khóa: Nghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngchuyên đề điện xoay chiều theo dạngđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhPhát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinTổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)Quản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)TÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲ