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Tài liệu Báo cáo khoa học: Steady-state and time-resolved fluorescence studies of conformational changes induced by cyclic AMP and DNA binding to cyclic AMP receptor protein from Escherichia coli ppt

Tài liệu Báo cáo khoa học: Platelet factor 4 disrupts the intracellular signalling cascade induced by vascular endothelial growth factor by both KDR dependent and independent mechanisms ppt

Tài liệu Báo cáo khoa học: Platelet factor 4 disrupts the intracellular signalling cascade induced by vascular endothelial growth factor by both KDR dependent and independent mechanisms ppt

... VEGF121 -induced proliferation downstream from the receptor. Presumablythis inhibition occurs at/or upstream from Raf1 and downstream from PLCc. We found the chemokine receptor CXCR3B, a putative PF-4 receptor [34], in ... arget of thetyrosine kinase activity of the KDR and to be involved inVEGF -induced DNA synthesis [31]. P LCc phosphorylationwas induced by VEGF165(10 ngÆmL)1)andVEGF121(10 ngÆmL)1) and ... efficiently inhibits the binding of VEGF165 to its receptor, but not that of VEGF121[26]. Thus, PF-4 may d isrupt the KDR-mediatedsignal transduction induced by VEGF121 by means of anunknown m echanism...
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Tài liệu Báo cáo khoa học: Multidentate pyridinones inhibit the metabolism of nontransferrin-bound iron by hepatocytes and hepatoma cells docx

Tài liệu Báo cáo khoa học: Multidentate pyridinones inhibit the metabolism of nontransferrin-bound iron by hepatocytes and hepatoma cells docx

... similarcharacteristics.Effect of chelators on uptake of NTBIThe effects of the chelators on NTBI uptake by hepatocytes and Q7 hepatoma cells were marked and similar. Allchelators decreased uptake to 20% or less of the ... contrast to NTBI uptake by Q7 cells, the uptake of Tf-bound Fe (TBI) by receptor- mediated endocytosis ismuch lower in hepatocytes in comparison with hepatomacells [23] and regulated by intracellular ... mobiliza-tion of NTBI; (c) to assess and compare the activity and toxicity of novel tetradentate and hexadentate pyridinoneswith bidentate DFP, whose toxicity may be due to thetransient formation of reactive...
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Tài liệu Báo cáo khoa học: A DExD⁄ H box RNA helicase is important for K+ deprivation responses and tolerance in Arabidopsis thaliana docx

Tài liệu Báo cáo khoa học: A DExD⁄ H box RNA helicase is important for K+ deprivation responses and tolerance in Arabidopsis thaliana docx

... zone (100 lm from the roottip) of Arabidopsis was used to measure the steady fluxprofile of K+.Table S1. Primers for amplifying the full-length cDNA,promoter and the length of other sequences ... White PJ & Broadley MR (2000) Mechanisms of caesium uptake by plants. New Phytol 147, 241–256.56 Hampton CR, Bowen HC, Broadley MR, HammondJP, Mead A, Payne KA, Pritchard J & White ... by low-K+, zeatin and cold treatments, and downregu-lated by high-K+stress. To further investigate the expression pattern of AtHELPS, pAtHELPS::GUS transgenic plants were generated. Histochem-ical...
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Tài liệu Báo cáo khoa học: TMPRSS13, a type II transmembrane serine protease, is inhibited by hepatocyte growth factor activator inhibitor type 1 and activates pro-hepatocyte growth factor pdf

Tài liệu Báo cáo khoa học: TMPRSS13, a type II transmembrane serine protease, is inhibited by hepatocyte growth factor activator inhibitor type 1 and activates pro-hepatocyte growth factor pdf

... hepatocyte growth factor activator inhibitortype 1 and activates pro-hepatocyte growth factorTomio Hashimoto1, Minoru Kato2, Takeshi Shimomura2 and Naomi Kitamura11 Department of Biological ... hepatocyte growth factor activator inhibitor type 2; HGF, hepatocyte growth factor; HGFA, hepatocyte growth factoractivator; HPAI, highly pathogenic avian influenza; IC50,the concentration of ... activities of some members of the TTSP familyare regulated by endogenous protease inhibitors, whichinclude Kunitz-type inhibitors and serpins [2]. Hepato-cyte growth factor activator inhibitor type...
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Tài liệu Báo cáo khoa học: MicroRNA-23a promotes the growth of gastric adenocarcinoma cell line MGC803 and downregulates interleukin-6 receptor pdf

Tài liệu Báo cáo khoa học: MicroRNA-23a promotes the growth of gastric adenocarcinoma cell line MGC803 and downregulates interleukin-6 receptor pdf

... regulatory effects of miR-23a on theexpression of IL6R by RT-PCR and western blot, and found an inverse correlation between miR-23a and IL6R expression at both the mRNA and protein levels. To confirm ... downregulation of IL6R by binding to its first conserved binding site,which then leads to the development of gastric adeno-carcinoma.Materials and methodsHuman cancer tissue samplesFresh-frozen ... tissue samples and matched normal gastric tissue samples were obtained from the Tumor Bank Facility of Tianjin Medical UniversityCancer Institute and Hospital and the National Foundation of Cancer...
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Tài liệu Báo cáo khoa học: Pyrimidine-specific ribonucleoside hydrolase from the archaeon Sulfolobus solfataricus – biochemical characterization and homology modeling doc

Tài liệu Báo cáo khoa học: Pyrimidine-specific ribonucleoside hydrolase from the archaeon Sulfolobus solfataricus – biochemical characterization and homology modeling doc

... guanosine and guanine, uridine and uracil, cyti-dine and cytosine were 12.2 and 6.2 min, 10.5 and 4.7 min,15.2 and 6.1 min, 6.8 and 4.2 min and 6.6 and 3.9 minrespectively. The amount of purine ... determined by integrating the peak of producednucleobase and converting this to the amount of nucleobase by means of a standard curve (amount nucleobase versuspeak area). In all of the kinetic and ... aprobe of 0.5 A˚ to assess the packing of the molecules.The evaluation of helix stability was carried out according to Facchiano et al. [48].Docking of ribose and Ca2+into the binding site of SsCU-NH...
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Tài liệu Báo cáo khoa học: Neuropeptide Y-family receptors Y6and Y7in chicken Cloning, pharmacological characterization, tissue distribution and conserved synteny with human chromosome region docx

Tài liệu Báo cáo khoa học: Neuropeptide Y-family receptors Y6and Y7in chicken Cloning, pharmacological characterization, tissue distribution and conserved synteny with human chromosome region docx

... regulation of appetite and circadian rhythm, by binding to G -protein coupled receptors. Mammals have five subtypes, named Y1,Y2,Y4,Y5 and Y6, and recently Y7has been discovered in fish and amphibi-ans. ... inligand affinity between Y7 and Y2may prove very use-ful for studies of ligand receptor interactions and 3Dmodeling, and we have previously been able to utilizedifferences between chicken and ... Chowdh-ary BP, Andersson L, Lundin L-G & Larhammar D(2000) Evolution of the neuropeptide Y receptor family:gene and chromosome duplications deduced from the cloning of the five receptor subtype...
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Tài liệu Báo cáo khóa học: Further insights into the assembly of the yeast cytochrome bc1 complex based on analysis of single and double deletion mutants lacking supernumerary subunits and cytochrome b pdf

Tài liệu Báo cáo khóa học: Further insights into the assembly of the yeast cytochrome bc1 complex based on analysis of single and double deletion mutants lacking supernumerary subunits and cytochrome b pdf

... transmembrane helix of subunit 8 and helices G and H1 of cytochrome b and between the N-terminus of subunit 8 and helix a of cytochrome b. The figure was constructed from the crystal structure of the yeast ... inner membrane of mitochondria [1,2].This respiratory enzyme catalyzes the transfer of electrons from ubiquinol to cytochrome c and couples the electrontransfer to vectorial proton translocation ... Assembly of the yeast cytochrome bc1complex (Eur. J. Biochem. 271) 1211transformation of Escherichia coli and isolation of plasmid DNA from bacterial cells were carried out by standardprocedures...
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Tài liệu Báo cáo khoa học: Phylogenetic relationships in class I of the superfamily of bacterial, fungal, and plant peroxidases pptx

Tài liệu Báo cáo khoa học: Phylogenetic relationships in class I of the superfamily of bacterial, fungal, and plant peroxidases pptx

... comparison of four representatives of class I of the superfamily of bacterial, fungal, and plant peroxidases. (A) N-terminal domain and (B) C-terminal domain of catalase–peroxidase from Haloarcula ... presentation of the inferred t ree with the use of anoutgroup (manganese peroxidase from Phanerochaete chrysosporiumbelonging to class II of this superfamily) presented to demonstrate theorder of evolutionary ... subjected to rearrangements cause d by thesemobile genetic elements. More data on the distribution of introns in ascomycetous genomes and more genomicsequences of KatGs are needed to analyse...
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Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc

Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc

... reader. To determine the kineticparameters of wild-type and W402F IPU for p anose,mixtures consisting of 4 lgÆmL)1enzyme and from 43 to 216 mMsubstrate, and 20 lgÆmL)1enzyme and 64 to 480 ... isopanose from pullulan, and isomaltose and glucose from panose, and the action patterns of the wild-type and the W402F enzymes were almost identical (Fig. 2).The kinetic study for W402F towards ... trans-glycosylation activity to produce isomaltose from maltose, and they are suggested to participate in the maltose-dependent induction of amylase s ynthesis along with otherundetected isomaltose-forming...
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