Tài liệu Báo cáo khoa học: Endotoxic activity and chemical structure of lipopolysaccharides from Chlamydia trachomatis serotypes E and L2 and Chlamydophila psittaci 6BC pdf

... prostaglandin E 2[10]. These reported data wereobtained on LPS of unknown chemical structure and of ill-deﬁned purity. Therefore, in this study we have comparedthe endotoxic activities of three chlamydial ... L2 and Chlamydophila psittaci 6BC Holger Heine, Sven Mu¨ ller-Loennies, Lore Brade, Buko Lindner and Helmut BradeResearch Center Borstel, Center for Medicine and Biosciences, Borstel, GermanyThe ... of known structure and deﬁned purity. Whereas the chemical struc-tures of the LPS from C. trachomatis serotype L2 and of Chl. psittaci 6BC have been reported previously [12,13], that of the...

Tài liệu Báo cáo khoa học: Different mechanisms for cellular internalization of the HIV-1 Tat-derived cell penetrating peptide and recombinant proteins fused to Tat docx

... same except that all incubations were performed at4 °C until the end of the ®xation procedure. For directdetection of ¯uorescein-labeled or rhodamine-labeledpeptides, cells were washed three ... was reached when three or fourmolecules of a 35-amino-acid Tat peptide (sequence 37±72)were chemically coupled to a large protein cargo. Despitethe presence of the basic region (sequence 49±57), ... time.Differences in the uptak e mechanism between the twoTat entities w ere also c on®rmed by t he temperaturedependence of the internalization process. As shown inFig. 5, ¯uorescein-labeled Tat peptide...

Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx

... protease. Moreover, theefﬁciency of generation of 30 K in the intermolecularreaction experiment seems less than that observed inthe experiment in which mGFP–calpain 7 was incu-bated, and we ... were detected by CBB staining.Coimmunoprecipitation assayOne day after HEK293T cells had been seeded, they weretransfected with 5 lg of expression plasmid DNA. After24 h, cells were harvested ... on glutathione–Sepha-rose beads. The beads were then pelleted by low-speed centrifuga-tion and washed with the lysis buffer. The cleared lysates (Input) and proteins in the pellets (pulldown...

Tài liệu Báo cáo khoa học: ATPase activity of magnesium chelatase subunit I is required to maintain subunit D in vivo ppt

... chelatase reaction requires ATP hydrolysis. Theobservations are consistent with earlier observations withpea magnesium chelatase where the magnesium chelatasereaction was demonstrated to be ... heterozygous plants carrying thesemidominant allele are pale green. It has been shown thatthe recessive mutations prevent transcription of the Xantha-hgene [24], while the semidominant alleles ... 0.5MNaCl,1Mimidazole, 6Murea). Two 2 mL fractions werecollected from the run-through, four 2 mL fractionswere collected from the wash and four 1 mL fractionswere collected from the elute. The collected...

Tài liệu Báo cáo khoa học: KIPase activity is a novel caspase-like activity associated with cell proliferation doc

... anemission wavelength of 440 nm. Background measurementsgenerated from the reaction completed in the absence of substrate were assessed and subtracted from experimentalvalues. Typical values ... synchronizecells, exponentially growing Jurkat 9.2 cells were seeded at adensity of 1 · 106per mL. The proliferative status of thecells was determined by assessing the extent of DNAsynthesis. ... have been generated by synthesizingaldehyde-modiﬁed substrates [30], we generated an equiv-alent form of the DPSD tetra peptide, termed DPSD-CHO. The ability of DPSD-CHO to inhibit KIPaseactivity...

Tài liệu Báo cáo khoa học: High activity of human butyrylcholinesterase at low pH in the presence of excess butyrylthiocholine pptx

... butyryl-cholinesterase at low pH in the presence of excess substrateremains to be elucidated.Keywords: butyrylcholinesterase; excess substrate activation;mutant enzyme; pH dependence; steady-state kinetics.Human ... (catalognumber MET-674B). All other chemicals, including buffercomponents, were of biochemical grade.Mutagenesis and expression of recombinant BuChEMutagenesis and expression were performed as describedpreviously ... transfected into CHO-KIcells by calcium phosphate coprecipitation. Stable cell lineswere selected in methionine sulfoximine. Expressed BuChEwas secreted from these cells and collected into serum-freemedium.Puriﬁcation...

Tài liệu Báo cáo khoa học: Adaptive changes in the expression of nuclear and mitochondrial encoded subunits of cytochrome c oxidase and the catalytic activity during hypoxia pptx

... physiologicallyrelevant hypoxia. The heme aa3content of SMP directlyrepresents the enzyme-associated heme. In both PC12 cells and macrophages a 38–55% decrease in heme aa3contentwas seen after 10 h of ... nm of 21.1 mM)1Æcm)1andthevaluesexpressedasmolÆmin)1Æmg)1protein. For the measurement of heme aa3 levels, the SMP weresolubilized in laurylmaltoside and heme aa3 levels were calculated ... A. The enzyme from hypoxia exposedcells exhibited altered subunit content as revealed by bluenative gel electrophoresis. There was a generalized decline inmitochondrial function that led to...

Tài liệu Báo cáo khoa học: 3T3-L1 adipocyte apoptosis induced by thiazolidinediones is peroxisome proliferator-activated receptor-c-dependent and mediated by the caspase-3-dependent apoptotic pathway doc

... Results are means ± SDs of three independentexperiments. **P < 0.01. (E) Rat adipocytes were treated withtroglitazone for 96 h at the indicated concentrations, and proteinswere detected ... intothe nucleus, and activates multiple genes, especiallyadipogenic genes [19]. In the mature adipocyte, PPARcregulates the expression of genes involved in lipidmetabolism [19]. Besides its ... Bcl-2 protein levels were reduced, and Baxtranslocation to the mitochondria was increased. Subsequently, cyto-chrome c release and caspase-3 cleavage were observed. TZD-inducedadipocyte apoptosis...

Tài liệu Báo cáo khoa học: Mixed lineage leukemia: a structure–function perspective of the MLL1 protein ppt

... lobes, one of which iscomposed of residues from the SET-I region and theother of residues from the SET-C and post-SETregions. Lysine 4 of histone H3 is inserted into a chan-nel, at the end of ... diverse cellular processes rang-ing from stem cell differentiation to metazoan devel-opment and cancer, there has been an intense interestin understanding how SET1 family enzymes regulateH3K4 ... crys-talline state by residues from the N-terminus of a sym-metry related molecule (Fig. 3B). It therefore remainsto be determined to what extent the observed confor-mation of the isolated MLL1 SET...

Tài liệu Báo cáo khoa học: Golgi reassembly stacking protein 55 interacts with membrane-type (MT) 1-matrix metalloprotease (MMP) and furin and plays a role in the activation of the MT1-MMP zymogen pdf

... normalized to Renilla lucifer-ase activity and is presented as relative luminescence units.Each transfection was performed in duplicate and two inde-pendent measurements were read per sample. All ... The western blot presentedis a representative of three independent experiments.Pro-MMP2 activation and zymographyHT1080 cells (1 · 105cells) in DMEM and 10% fetal bovineserum were transfected ... numericalvalues are shown as the mean ± SEM. The graphs pre-sented are representative of at least three experiments.ImmunoprecipitationHT1080 cells (2 · 105per well in a six-well plate) weretransfected...

Xem thêm