... Wealso thank the staff members at beamline ID14-1 for their skilled assistance, and B. Hillebrant-Chellaoui atVTT for skilful help with the TaLcc1 purification.Roal Oy and the Academy of Finland ... dataKinetic constants (Kmand Vmax) for rMaL, TaLcc1 andThL were determined on 2,6-DMP, syringic acid, andmethyl syringate, at both pH 4.5 and pH 6.0, in 25 mM suc-cinate buffer and 40 mM Mes ... change in absor-bance over 5 min. All of the measurements were performedin duplicate, with eight substrate concentrations (0.008–1.333 mM for 2,6-DMP and syringic acid; 0.008–2.800 mMfor...