Tài liệu Báo cáo khoa học: Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells pdf
Tài liệu Báo cáo khoa học: Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells pdf
... enrichment in iNOS activity within the particulate fraction of epithelial cells. Apical distribution of iNOS in intestinal epithelial cells To get more insight into the localization of iNOS in intestinal ... association of iNOS in vivo In order to determine the distribution of iNOS in intestinal epithelial cells in vivo, experiments were con-ducted in mice injected with bacterial flagellin. Flagel-lin activates ... pathogens,which, in intestinal cells, could be bacteria present in the intestinal lumen. This hypothesis is supported bypositioning of iNOS on the apical side of intestinal crypts. Recently PDZ-binding a2⁄...
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Tài liệu Báo cáo khoa học: Pyruvate reduces DNA damage during hypoxia and after reoxygenation in hepatocellular carcinoma cells pptx
... level of ROS in the medium of HepG2 cells exposed to hydrogen peroxide. HepG2 cells were incu-bated with 5.5 mM glucose in the presence of 0.8 mM pyruvate,and exposed to increasing doses of hydrogen ... notshown). This is in line with our previous work [29]indicating enhancement of glycolysis activity in thepresence of pyruvate. Increased glycolysis might thusmaintain the ATP supply during oxygen ... effects of pyruvate on gluta-thione content during hypoxia, we examined pyruvatemetabolism using13C-NMR. The distribution of 13Clabeling was analyzed after incubation of HepG2 cells in a culture...
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Tài liệu Báo cáo khoa học: Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a gene encoding a conserved bacterial type I SPase pdf
... (CAF) [30]: during the first step of the labelingreaction the e-amino groups of lysine are specificallyconverted to homoarginine. In a second step, the freeamino terminal groups of the peptides ... function of this protein com-plex, the subunits had to be characterized in detail.The N-terminal end of P90 was determined by Edmandegradation. This protein begins with an arginine atamino-acid ... is a type ISPase activity in M. pneumoniae, we determined theN-terminus of P40 as it appears in protein extracts of M. pneumoniae.Determination of the N-terminus of P40 by Edmandegradation...
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Tài liệu Báo cáo khoa học: "The Structure of User-Adviser Dialogues: Is there Method in their Madness?" pdf
... STRUCTURES 227 DISTRIBUTION OF NON-PRONOMINAL AND PRONOMINAL NOUN PHRASES Non-pronominal noun phrases play a role in indicating and realizing topic shifts in a dialogue. Since new subdialogues ... realizing topic continuity in a dialogue. Since new topics are introduced at the beginning of new subdialogues and developed in the following sentences, one can predict that pronominal noun ... validating the derived dialogue structure by three independent converging analyses: a) the distribution of non- pronominal and pronominal noun phrases b) the distribution of antecedents of pronominal...
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Tài liệu Báo cáo khoa học: Electrical properties of plasma membrane modulate subcellular distribution of K-Ras ppt
... replace-ment of lysine residues by arginine or d-lysine residues in the polybasic domain of K-Ras does not interferewith plasma membrane localization of this protein[30], suggesting that binding of K-Ras ... incubated in the presence of monensin,plasma membrane targeting of K-Ras was notaltered (Fig. 7), thus ruling out a possible role of H+ in intracellular transport and distribution of this pro-tein. ... modifications in shape of neuraminidase-treated cells (results not shown).Quantification of neuraminidase effect on subcellular distribution of K-Ras (Fig. 4B) suggested that theincrease in number of cells...
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Tài liệu Báo cáo khoa học: Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways doc
... and anaerobic condi-tions, in the range of 0.6 lmolÆmg)1Æmin)1. No evidence wasobtained for redox cycling of FMN. The amino acidresidues involved in the FMN binding site are absolutelyconserved ... M15-pQEidi2.Preparation of the recombinant Idi-2 proteinThe recombinant E. coli strain M15-pQEidi2 was grown in Luria-Bertani broth containing ampicillin (180 mgÆL)1)andkanamycin (50 mgÆL)1). Cultures were incubated ... binding site suggests that the substrates couldbe bound in close proximity to the isoalloxazine moiety. In the absence of direct evidence for the involvement of a redoxprocess it is tempting...
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Tài liệu Báo cáo khoa học: Molecular basis of glyphosate resistance – different approaches through protein engineering doc
... forglyphosate activity is accompanied by improved bind-ing to longer ligands (up to five atoms in the mainchain) and retained binding to shorter ligands [55]. Of the 21 changes in the evolution of R7 ... d-amino acid oxidase [47]. In B. subtil-is, GO is involved in biosynthesis of the thiazolemoiety of thiamine pyrophosphate (vitamin B1). Thisreaction requires the direct transfer of the imine ... An in silico dockinganalysis of glyphosate binding at the GO active siteshowed that glyphosate is bound in the same orienta-tion as inferred for glycine (with the phosphonate moi-ety pointing...
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Tài liệu Báo cáo khoa học:Tyrosine phosphorylation of tau regulates its interactions with Fyn SH2 domains, but not SH3 domains, altering the cellular localization of tau ppt
... thattau interacts with Fyn-SH2. Proteins harbouring SH2domains bind to phosphorylated tyrosines on their tar-get proteins, thereby coupling the activity of tyrosinekinases with intracellular ... interacting pro-teins. Seven such motifs are present in the proline-richdomain of the longest isoform of tau in the humancentral nervous system (CNS). Two distinct PXXPmotifs in tau, residing ... binding of tau to Fyn-SH2,thus suggesting that the interaction between tau andFyn-SH2 is enhanced by increased tyrosine phosphory-lation in cells. Tyr18 of tau plays an important role in theinteraction...
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Tài liệu Báo cáo khoa học: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases ppt
... observe any trace of themovements on the His98 residue in our MaL ⁄ rMaLFig. 2. Stabilizing carbohydrates of TaLcc1. Domain A is presented in blue, domain B in green, and domain C in yellow. (A) ... mutation, suggesting the importance of the carboxylic group for the catalytic activity [46]. In addition, Phe427 in rMaL (Val428 in TaLcc1) mightbe involved in placing substrate molecules into the cor-rect ... 507–513.50 Krissinel E & Henrick K (2005) Detection of proteinassemblies in crystals. In Lecture Notes in Computer Sci-ence (Berthold MR ed), pp 163–174. Springer-Verlag,Berlin.51 Krissinel E...
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Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt
... determined the X-ray crystal structure of a truncated form of importin-a lacking the importin-b binding domain, bound to a CLIC4NLS peptide. The NLS peptide binds to the major binding site in anextended ... Theimportin-b binding domain is known to have anautoinhibitory function, whereby an internal NLS-likesequence competes for the importin-a binding site,reducing binding affinity for cargo proteins ... BL21 (DE3) cells and grown at37 °C overnight in Luria–Bertani medium containing30 lgÆmL)1 of kanamycin. Flasks of 400 mL of Luria–Bertani medium (30 lgÆmL)1kanamycin) were then inocu-lated...
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