Tài liệu Báo cáo khoa học: Thermodynamic analysis of porphyrin binding to Momordica charantia (bitter gourd) lectin pptx

... Thermodynamic analysis of porphyrin binding to Momordica charantia (bitter gourd) lectin Nabil A. M. Sultan, Bhaskar G. Maiya* and Musti J. SwamySchool of Chemistry, University of Hyderabad, ... IndiaOwing to the use of porphyrins in photodynamic therapy forthe treatment of malignant tumors, and the preferentialinteraction of le ctins with tumor c ells, s tudies on lectin porphyrin ... possibleapproach to overcome this limitation is t o conjugate the porphyrin to another agent that can direct it to the tumortissue. In view of the known ability of certain lectins to preferentially...

Tài liệu Báo cáo khoa học: Subproteomics analysis of Ca2+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle pdf

... VIIID12 to calsequestrin, mAb X IIC4 to sarcalu-menin, mAb IIH11 to the fast SERCA1 isoform of thesarcoplasmic reticulum Ca2+ATPase, mAb IIID5 to thea1-subunit of the dihydropyridine receptor, ... identiﬁcation of the t ranscription cofactor vestigial-like p rotein 2 (UniProt AC: Q8BGW8; UniProt ID:VGL2_MOUSE). This cofactor of the t ranscription en-hancer factor TEF-1 appears to be a new ... fractionation protocols maydistort comparative immunoblotting data, it was of interest to re-examine the fate of cals equestrin by studying the entirecomplement of key Ca2+ -binding elements...

Tài liệu Báo cáo khoa học: Proteomic analysis of dopamine and a-synuclein interplay in a cellular model of Parkinson’s disease pathogenesis docx

... Department of Structural and Functional Biology, and Centre of Neuroscience, University of Insubria, Busto Arsizio, Italy2 Department of Biotechnology, University of Verona, Italy3 Department of Clinical ... affected the levels of four proteins [stathmin 1, glutathione S-transferase(GST)p, Ran 1 binding protein and C1q binding pro-tein], related to cell signaling, apoptosis and cytoskele-ton regulation ... either cytoskeleton components orregulatory proteins were suggested to be linked to early stages of PD pathogenesis [26]. Interestingly, inour model, dopamine induces an increase of the actinbundles...

Tài liệu Báo cáo khoa học: Kinetic analysis of effector modulation of butyrylcholinesterase-catalysed hydrolysis of acetanilides and homologous esters pdf

... actual binding site of serotonin cannot be inferredfrom these results. Although serotonin can bind to theactive site binding locus (W82), binding to PAS cannotbe ruled out. If serotonin binds ... varied from 0.1 to 15 mm,that of serotonin from 0.05 to 15 mm, and that of benzalkonium from 0.05 lm to 0.5 mm.The type of inhibition or activation was determined byvisual inspection of velocity ... ligands were found to be reversibleinhibitors of the BuChE-catalysed hydrolysis of o-nit-roacetanilide (o-NAC), tyramine was found to be anactivator. However, kinetic analysis of these inhibitingor...

Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll ﬂuorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc

... FEBShave attributed both of the two latter phases to thereduction of the acceptor side of PSII [9,12,13], or morespeciﬁcally to the reduction of two distinct plastoqui-none (PQ) pools [8,14,15]. ... rise from F0 to maximal level (Fmor P), related to a series of succes-sive events that lead to the progressive reduction of the quinone molecules located on the acceptor side of PSII [3].The ... chose to measure FI in thylakoids in the absence of additives orin the presence of 1 lm DCMU or dPQ over a range of temperature from 15 °Cto25°C. The range of tem-perature was set on the basis of...

Tài liệu Báo cáo khoa học: Thermodynamic characterization of interleukin-8 monomer binding to CXCR1 receptor N-terminal domain ppt

... elicit their function by binding receptors of the G-protein-cou-pled receptor class, and the N-terminal domain (N-domain) of the receptoris one of the two critical ligand -binding sites. In this ... differencesin binding may be due to binding of N-loop residues to the receptor N-domain.The structure of IL-8 is known, and the structuralbasis for its function has been well studied [8–17]. Thereceptor ... monomer to the CXCR1 N-domain peptide.We used ITC to determine the enthalpy (DH),entropy (DS), and the free energy (DG) of binding of monomeric IL-8 to the receptor CXCR1 N-domain.The binding...

Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

... BMAL1 and the bin ding to the E-box.Substitution of Arg65 for Ala abolished the interaction of DEC1 with BMAL1 and the binding of DEC1 to the E-box.However, substitution of His57 for Ala did ... interaction of DEC1 with BMAL1 has been suggested a s one of themolecular mechanisms of the suppression [Honma, S.,Kawamoto, T., Takagi, Y., Fujimoto, K., Sato, F.,Noshiro, M., Kato, Y. & ... 10 ng) of FLAG-DEC1 expression vector were co-transfected with pDEC1-E-ABC-TK, together with expression vectors for CL OCK and BMAL1. The total amount of transfected DNA wasadjustedtothesamevalue,ineachexperiment,bytheadditionofanemptyvector...

Tài liệu Báo cáo khoa học: Control analysis as a tool to understand the formation of the las operon in Lactococcus lactis doc

... 2005 FEBS 2303B. Koebmann et al. Control analysis of the las operonControl analysis as a tool to understand the formation of the las operon in Lactococcus lactisBrian Koebmann, Christian Solem ... synthesis:phosphofructokinase, pyruvate kinase, and lactate dehy-drogenase. J Bacteriol 175, 2541–2551.9 Andersen HW, Solem C, Hammer K & Jensen PR(2001) Twofold reduction of phosphofructokinase ... control of a synthetic promoter.The relative PFK activity in strain CS1929 was found to increase to over 160% of the wild-type level,whereas the relative LDH activity was reduced to 80% of the...

Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf

... a-helix F inorange and parts of b-sheet A in pink. Backbone atoms of the s1A/hF-loop (residues D127 to E130) and of the top of hF and the hF/s3A-loop (residues T146 to L154) are shown in CPK colours. ... Mechan-isms of conversion of plasminogen activator inhibitor 1 from asuicide inhibitor to a substrate by monoclonal antibodies. J. Biol.Chem. 277, 43858–43865.Ó FEBS 2003 Mutational analysis of PAI-1 ... AndreasenLaboratory of Cellular Protein Science, Department of Molecular Biology, Aarhus University, DenmarkThe serpin plasminogen activator inhibitor-1 (PAI-1) is afast and speciﬁc inhibitor of the...

Tài liệu Báo cáo khoa học: Fluorescence analysis of the Hansenula polymorpha peroxisomal targeting signal-1 receptor, Pex5p pdf

... to local Trp ﬂexibility andresonance energy transfer among Trp residues.Molecular modeling of HpPex5p conjugated to peptide L2 To investigate the binding of the dansylated PTS1 peptideL2 to ... the cytosol (pH 7.2), whereas the cargo isreleased from HpPex5p upon dissociation of the proteininto monomers in the slightly acidic peroxisomal matrix.Cytosolic binding of PTS1 proteins to oligomeric ... conformational properties of HpPex5p, the PTS1receptor of the yeast H. polymorpha. Fluorescence emissionspectra revealed a pH dependence of Trp ﬂuorescence of HpPex5p, indicative of pH driven changes...

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