Tài liệu Báo cáo khoa học: Evolutionary relationships of the prolyl oligopeptidase family enzymes docx

... alignment of studied POP family enzymes. Ó FEBS 2004 Evolutionary relationships of the POP family enzymes (Eur. J. Biochem. 271) 2715 Evolutionary relationships of the prolyl oligopeptidase family enzymes Jarkko ... ancestor of life. With respect to the studied enzymes of the POP family, POP seems to be the most conserved enzyme. Ten conserved amino acids werefound at the active site of the enzyme of each of the ... IV;evolution; oligopeptidase B; prolyl oligopeptidase. The prolyl oligopeptidase family of serine proteases (clanSC, family S9) includes a number of peptidases, from which prolyl oligopeptidase...

Tài liệu Báo cáo khoa học: Evolutionary divergence of valosin-containing protein ⁄cell division cycle protein 48 binding interactions among endoplasmic reticulum-associated degradation proteins ppt

... substitution of these two arginines. We map the site of binding of Ube4b to the N-domain of VCP andshow that it competes for this site with Hrd1. Finally,we investigate the evolutionary divergence of the ... mediatingbinding both on the Ube4b–Ufd2p side [8] and on the VCP–Cdc48p side.We then investigated whether the binding of each of these VBM-containing proteins to the N-domain of VCP is disrupted ... a local mouse database. The sig-niﬁcance cutoff was set at 1000 to allow for the shortness of the search string. In the absence of a protein sequence, the existence of a homologous gene was...

Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

... [26]. These ﬁndingslead to the hypothesis that Fe-bound ApeSOD mimics the product-inhibited form and the shift of Tyr39 sup-presses the release of the peroxide product. This maybe one of the ... ligand,NE2 of His31, bound to the metal, in the company of a water oxygen, from the apical positions. The manga-nese was only 0.06 A˚out of the equatorial plane(Table 3). The angles around the metal ... inhibi-tion of the binding of the superoxide substrate and the other to product inhibition by hydrogen peroxide.There are two mechanisms proposed for the reactioncycle of Mn ⁄ Fe-SOD: the 5-6-5...

Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt

... man-ganese in the transcriptional regulation of the nrdoperon [35] may not be considered in the light of the results obtained in the present study. Rather, the involvement of manganese in the in vivo ... to the catalytic site of the R1E subunit. Asreported in the Results, upon completion of substrateconversion, the radical is then rapidly passed backfrom the R1E subunit to the tyrosyl of the ... RNR and that of other class 1RNRs or, instead, is a result of the interaction of the R2F with the inactivated (oxidized) form of the R1E.We consider the ﬁrst option unlikely. Under these cir-cumstances,...

Tài liệu Báo cáo khoa học: Reductive nitrosylation of ferric human serum heme-albumin docx

... 4 shows the dependence of the molar fraction of HSA-heme-Fe(II)-NO (i.e. Y) on the NO concentra-tion (i.e. [NO]). The value of Y increased linearly with the NO concentration, reaching the maximum(= ... [44]:kobs¼ konÂ½NOþkoffð2Þ The value of K (= koff⁄ kon; reaction (a) in Scheme 1)was determined from the dependence of the molar fraction of HSA-heme-Fe(III)-NO (i.e. Y) on the free NO concen-tration ... dependence of the molar fraction of HSA-heme-Fe(III)-NO (i.e. Y) on the NO concentration (i.e.[NO]) is shown in Fig. 1. The analysis of data accord-ing to Eqn (3) allowed the value of K (= 1.5...

Tài liệu Báo cáo khoa học: Crystal structures of the regulatory subunit of Thr-sensitive aspartate kinase fromThermus thermophilus pdf

... EC 2.7.2.4) is an enzyme thatcatalyzes the ﬁrst committed step, the phosphorylation of the c-carboxyl group of aspartate, of the biosyntheticpathway of the aspartic acid group amino acids Lys,Thr, ... and B)sections of the structure are not seen on the map,probably owing to disorder of these regions. The over-all geometry of the model according to the procheckprogram [21] is of good quality, ... in the absence of Thr [20]. Therefore, a sim-ilar conformational change is expected for these two enzymes, depending on the presence or absence of Thr.In CgAK, mutations of the residues in the...

Tài liệu Báo cáo khoa học: Limited suppression of the interferon-b production by hepatitis C virus serine protease in cultured human hepatocytes pptx

... that the activation of IRF-3 by M-pIC treatment is mediatedby the TLR3 ⁄ TRIF signaling pathway. We obtainedfurther evidence by examining the status of the phos-phorylation and dimerization of ... cells. First, weconﬁrmed the effect of NS3-4A on the activation of the IFN-b gene promoter by the Cardif exogenouslyexpressed in PH5CH8 cells. The results of the lucif-erase reporter assay revealed ... sup-press the activation (1000-fold induction) of the IFN-bgene promoter by TRIF (Fig. 7A). This result was alsosupported by the results of the dimerization analysis of IRF-3 (Fig. 7B). Furthermore,...

Tài liệu Báo cáo khoa học: Kinetic characterization of the ﬁrst step of the ribozyme-catalyzed trans excision-splicing reaction docx

... Kdis the equilibrium dissociation constant of the 5¢ exon mimic, h is the fraction of 5¢ exon mimic boundto the ribozyme, and [ribozyme]uis the concentration of unbound ribozyme in the reaction.Measurement ... and ﬁtting to the equation:kobs¼ k1½EþkÀ1:Measurement of the dissociation constant, KdP of the ribozyme–product complex The equilibrium dissociation constant KdP of the 5¢ exonmimic ... maintain the integrity of the bound species during gel electrophoresis, the gel and the running buffer were made of H10Mg bufferand were prewarmed to 44 °C before the samples wereloaded. The bound...

Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx

... according to the residue in the mid-point of the respective loop, as shown in Fig. 2. To the east of the active site the 37- and 60-loops border the S2¢ pocket of the proteinase. The observed ... below). The southern boundary of the active site cleft of DESC1 is formed by the 145autolysis loop. The backbone of this loop differs mark-edly from the other serine proteinases, making the act-ive ... revealsthat the most similar regions of these proteinases medi-ate interaction of the two b-barrels, formation of the catalytic machinery and structures required for binding of the main chain of the...

Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc

... determines the shape of the active site entry. (e) The structure of the active site isessentially identical with the active sites of the MshBand LpxC proteins. The conservation of catalyticallyimportant ... orients the C-terminal turn of the helix nearly perpendicularly to the rest of the helix. This turn of a5 is connected with a6-helix via along loop (residues 180–193) which, on the basis of the B-values ... formation. Sections (6 A˚thick) of the protein surface that illustrate the shape and size of the active site. The sphere represents the zinc ion. (A) The main body of the active site cavity is formed...

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