Tài liệu Báo cáo khoa học: NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles doc

... VpUcytFEBS Journal 276 (2009) 6560–6575 ª 2009 The Authors Journal compilation ª 2009 FEBS 6575 NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine ... (2002) Structural investigation of the binding of a herpesviral protein to the SH3 domain of tyrosine kinase Lck.Biochemistry 41, 5120–5130.M. Wittlich et al. NMR structure of micelle-associated HIV-1 ... implications of the observed VpUcytstructureOne of the most studied activities of VpU is the induc-tion of CD4 degradation in the endoplasmatic reticu-lum of infected CD4+cells. Only the cytoplasmic domain...

Tài liệu Báo cáo khoa học: "Using Structural Information for Identifying Similar Chinese Characters" pdf

... comparing the actual Cangjie codes. 2.3 Engineering the Original Cangjie Codes Although useful for the sake of designing input method, the simplification of Cangjie codes causes difficulties ... similar in their pronunciations or in their internal structures are useful for computer-assisted language learning and for psycholinguistic studies. Al-though it is possible for us to employ ... directly useful; for instance, the common part of the characters in the right group in the second row in Figure 3 appears in different places in the characters. Lexicographers employ radicals of...

Tài liệu Báo cáo khoa học: NMR structure of AII in solution compared with the X-ray structure of AII bound to the mAb Fab131 pptx

... Structural determination of these proteins has progressed slowly [6], mainly because of technical difﬁculties in purifying and handling integralmembrane proteins. The instability of these proteins ... intermediate values (6.4 Hz) of the backbone couplings for Tyr4 and His6 are possibly the result of twisting or bending in the middle of the amino-acidsequence of the hormone [49].Structure calculations ... calculations and analysis The presence of conformational averaging in linear peptidescan complicate the calculation of singular structures. In particular, the use of intraresidue and sequential NOEs,which...

Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx

... although this recombi-nant pulchellin binding chain does not correspond toany of the four B-chains presented here.Purification of four pulchellin isoforms fromA. pulchellus seedsUsing a combination ... some interesting varia-tion in the flanking regions around the second sugarbinding pocket that could in uence the P III ⁄ PIVspecificity and binding properties. Of particular interestis the substitution ... previously published observa-tions, we predict residues comprising the two sugarbinding pockets in the pulchellins (Fig. 3). In order to compare the similarity of the A- andB-chains of the four isoforms,...

Tài liệu Báo cáo khoa học: Transduced human PEP-1–heat shock protein 27 efﬁciently protects against brain ischemic insult pptx

... membrane-translocating proteins.These peptides and fusion proteins are internalizedinto cells by endocytosis. Thus, cell ﬁxation should beavoided in studies of protein transduction into livingcells ... primary neuro-nal cells. The intracellular stability of transduced PEP-1–HSP27 fusion protein in astrocytes is shown in Fig. 4C. The PEP-1–HSP27 fusion protein was addedto the culture medium at ... allow the delivery of exogenous protein into living cells. These include carrier peptides derivedfrom the HIV-1 Tat protein, Drosophila Antennapedia(Antp) protein and herpes simplex virus VP22 protein [17]....

Tài liệu Báo cáo khoa học: It is all about resolution Meeting report based upon presentations at the 10th International Global BioMillennium 2006 symposium on molecular cell biology (Tbilisi, Georgia) docx

... aswas discussed by Javier F. Caceres (Edinburgh, UK). The trans-acting factors involved include both serine-arginine (SR) and heterogeneous nuclear ribonucleo- protein (hnRNP) A ⁄ B proteins, with ... rapidly shuttles between the cytoplasm and the nucleus. A region in the C-terminus of STAT2controls its speciﬁc export in the absence of interferon.STAT1 also shuttles in the absence of interferon, ... use of two-photon microscopy for studying how neuronaldendrites form in adult-born neurons that continuouslydevelop into the olfactory bulb. Lentivirus green ﬂuor-escent protein- labeled neurons...

Tài liệu Báo cáo khoa học: Preliminary molecular characterization and crystallization of mitochondrial respiratory complex II from porcine heart ppt

... catalyzes the oxidation of succinate to fumarate in the mitochond-rial matrix as succinate dehydrogenase. Succinate oxi-dation is coupled to reduction of ubiquinone toubiquinol at the mitochondrial ... are summarized in Table 1.As the porcine genome sequence had not been fullyreleased at the time of our study, we measured the accurate mature amino acid sequences of the fourcomplex II subunits ... chickenheart by Huang et al. [21]. It contains four nonidenti-cal subunits: the FAD-binding protein or ﬂavoprotein(Fp, 68 kDa, 622 amino acids), the iron–sulfur protein (Ip, 29 kDa, 252 amino acids),...

Tài liệu Báo cáo khoa học: Cell surface nucleolin on developing muscle is a potential ligand for the axonal receptor protein tyrosine phosphatase-r ppt

... RGG domain of nucleolin.Nucleolin is present on the surface of developingmuscle tissue In order to function as a potential ligand for PTP r,nucleolin must be present on the surface of the ... and lactoferrin bindnucleolin and perturb PTPr binding todeveloping muscleTo ascertain whether FN3d–AP binds to nucleolin in chick muscle tissue, we tested whether the pentamericpseudopeptide ... surface nucleolin, with the binding sitelocated within the C-terminal RGG domain of nucleo-lin [52]. We preincubated sections of E10 chickembryos with 0.5 mgÆmL)1lactoferrin. This preincuba-tion...

Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the ﬁlamentous fungus Trichoderma reesei ppt

... chromatography.During the search for and conﬁrmation of the N-ter-minus and C-terminus of the protein, many other pep-tides were also analyzed, and the results conﬁrmedmost of the remaining deduced amino ... N-termini of the mature secreted enzymes coincide with the N-terminus of the intracellular enzyme (Fig. 1). The segments in the N-terminal portion around the copper ligandamino acids of the active ... Cleavage of structural proteinsduring the assembly of the head of bacteriophage T4.Nature 227, 680–685.21 Kerovuo J, Laureus M, Nurminen P, Kalkkinen N &Apajalahti J (1998) Isolation, characterization, ...

Tài liệu Báo cáo khoa học: Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum) ppt

... or into the substrate proteins. The puri-ﬁed recombinant PsCIPK protein (0.5 lg) alone forautophosphorylation or in the presence of the substrate(PsCBL or casein) was incubated in the kinase ... calcium-binding protein) [7,8]. CBL proteins contain fourCa2+-binding EF hand motifs [9] and functions byinteracting and regulating a group of Ser ⁄ Thr pro-tein kinases called CBL-interacting ... electro-transfer of the proteins onto PVDF mem-brane. The proteins on the membrane were denatured andthen gradually renatured by incubating in 6 m guani-dine ⁄ HCl (made up in HSM buffer: 25 mm...

Xem thêm