... probes bis-ANS and SYPRO Orange in complex with phosphorylated and non-phosphorylated HSL. HSL was phosphorylated by PKA, dialysed, and mixed with bis-ANS, and spectra were recorded at an excitation ... 266, 1134 1–1 1346.5 Granneman JG & Moore HP (2008) Location, location: protein trafficking and lipolysis in adipocytes. TrendsEndocrinol Metab 19, 3–9 .6 Granneman JG, Moore HP, Granneman RL, ... between bis-ANS andATP, the binding assay provided only reliable perfor-mance in a short range of the bis-ANS concentrationstested in Fig. 2, and only 1 lm, 1.5 lm and 2 lm bis-ANS provided...