... constructs, full-length s-DAPK-1, s-DAPK-1Dtail, and s-DAPK-1G296AR297A, which allowed usto evaluate whether the tail contributes to the functionof the s-DAPK-1 protein. Unlike DAPK-1, s-DAPK-1does ... that the depletion of the Myc tag only changes the size of the upper band, and that therefore the upper band repre-sents the ‘full-length’ s-DAPK-1.Two s-DAPK-1 deletion mutants, Flag-AO (Anky-rin ... 2A). Upon trans-fection, the same lower protein band was observed in the Flag–s-DAPK- 1- and the Flag–s-DAPK-1-Myc-transfected cells, whereas the upper band in the Flag–s-DAPK-1 transfection...