Tài liệu Báo cáo khoa học: Accessibility changes within diphtheria toxin T domain when in the functional molten globule state, as determined using hydrogen⁄deuterium exchange measurements pdf
Tài liệu Báo cáo khoa học: Accessibility changes within diphtheria toxin T domain when in the functional molten globule state, as determined using hydrogen⁄deuterium exchange measurements pdf
... surprising that TH8 is in the corebecause it is buried in the structure. Within the whole toxin, TH5 is partly covered by the C-terminal part of the receptor (R) domain [2,3]. Within the isolated T domain, ... to the functional state, which initi-ates the translocation of the catalytic domain. Here, the data allowed identification of the core of the pro-tein in the N state and the evolution of the overallstructure ... functional molten globule state at acidic pH. In the native state, the a-helices TH5 and TH8 are identified as the core of the domain. Based on the high-resolution structure of the T domain, wepropose...
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Tài liệu Báo cáo khoa học: Analyzing changes of chromatin-bound replication proteins occurring in response to and after release from a hypoxic block of replicon initiation in T24 cells pptx
... delta in mammalian replication, with the fast sedimenting Triton-extracted fraction. In a first experiment we employed this protocol therebydemonstrating that important components of the ‘classical’prereplication ... nuclei by Triton X-100 contain-ing buffer, and in fraction 3, the chromatin fraction, allproteins remain that resist Triton extraction. We supposedthat the latter fraction included, besides the commonchromatin ... thirdof the gradient and attributable to short chains originatingfrom newly initiated replicons. In the course of further25 min of reoxygenated growth, the incorporation of[3H]Thd still increased,...
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Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx
... hasreceived great attention over the past years using knockout and transfection technologies. In markedcontrast, studies addressing the effect that iron loadingof hepatic cells has on the expression ... class I molecule localizes in the cytoplasm ofhuman epithelial cells of the gastrointestinal tract andliver, two central organs in the regulation of ironmetabolism [20,21]. After their synthesis ... family. In this context, it is import-ant to draw attention to the fact that HepG2 cellsgrown in iron rich media: (a) induced coordinated changes in the expression of the transferrin receptorand...
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Tài liệu Báo cáo khoa học: High activity of human butyrylcholinesterase at low pH in the presence of excess butyrylthiocholine pptx
... causes the basicity of the histidine to increase, so that it is able toaccept/abstract the Oc proton from the catalytic serine.Transferring a proton from the catalytic serine to the catalytic histidine ... recipient for the serine proton is the catalytichistidine. Most models use the histidine to shuttle protons:first, between the serine and the leaving group; and thenbetween the attacking water ... Thisactivity is titratable, with pKavalues of 6.0–6.6, suggestingthat the catalytic histidine is protonated at pH 5. The existence of activity when the catalytic histidine is protonatedindicates...
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Tài liệu Báo cáo khoa học: Adaptive changes in the expression of nuclear and mitochondrial encoded subunits of cytochrome c oxidase and the catalytic activity during hypoxia pptx
... about 50% in macrophages exposed to hypoxia,thus indicating a major change in the CytOX content in these cells. These results suggest that the CytOX activity in the two cell types is differentially ... alsoseen that the level of ATPase complex as determined byimmunoblotting with antibody to the F1ATPase did notchange under these conditions (Fig. 3B). Quantification of the blots shows that the ... known inhibitors of hemebiosynthesis, these agents also elicit nonspecific and toxiceffects in animals. To ascertain that the effects of theseagents, on the catalytic activity and subunit compositionof...
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Tài liệu Báo cáo khoa học: Proteomic analysis of dopamine and a-synuclein interplay in a cellular model of Parkinson’s disease pathogenesis docx
... were collected in the supernatant and theirconcentration was determined using the Bio-Rad ProteinAssay (Bio-Rad). All experiments were run in triplicate. In this way, three independent samples ... ofa-synuclein are present, either as a consequence oftransfection or of dopamine treatment (Fig. 1), sug-gesting that a-synuclein could at least contribute to the deactivation of this cascade.Dopamine ... known to induce apoptosis [43] and the results obtained in the present study are in agree-ment with this finding (Fig. 5). Although both anti-apoptotic and pro-apoptotic properties were attributedto...
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Tài liệu Báo cáo khoa học: A strategy for discovery of cancer glyco-biomarkers in serum using newly developed technologies for glycoproteomics ppt
... allowingidentification of the core domain. Then, it is necessaryto confirm that the protein has the target O-glycosyla-tion by an alternative method, although it remainsdifficult to confirm the O-glycan ... method to identify carrierproteins with the specific lectin epitope. Using this strategy, we have identi-fied many glycoproteins containing glycan structures that are altered in cancer cells. These ... livercontaining the protein (gene) ID, protein name, glycosy-lated sites, and kinds of lectins used to capture glyco-peptides. In the next phase, we will provide additionaldata for other tissues...
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Tài liệu Báo cáo khoa học: Synchronization of Ca2+ oscillations: involvement of ATP release in astrocytes pdf
... generatedinducible transgenic mice that express a dominant-neg-ative SNARE domain selectively in astrocytes to block the exocytotic events in astrocytes. Using the trans-genic mice, they demonstrated ... gliotransmitters through whichastrocytes can actively regulate synaptic transmission.ATP differs from glutamate in that it inhibits ratherthan potentiates synaptic transmission. Thus, wehypothesize ... should be noted thatastrocytes themselves evoke the propagating Ca2+transients. As shown in Fig 1Ba, which represents changes in [Ca2+]i in the neuron–astrocyte cocultures,astrocytes and neurons...
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Tài liệu Báo cáo khoa học: Exposure of IgG to an acidic environment results in molecular modifications and in enhanced protective activity in sepsis doc
... data strongly argue that the therapeutic effect of the modified IVIg was not due tobetter neutralization of the injected LPS: first, the binding of the preparation to LPS in its two formswas ... saltconcentration in the buffer. In contrast, this interac-tion was mostly independent of the salt concentration in the case when the low-pH buffer-exposed Z2 wasleft to bind with its target antigen (Fig. ... was used for the calculation of the kineticrate constants. Calculations were performed by globalanalysis of the experimental data using the kinetic modelsincluded in the software, fitting the...
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Tài liệu Báo cáo khoa học: Disulfide bridge regulates ligand-binding site selectivity in liver bile acid-binding proteins ppt
... whereas the bound GCA mostly in uenced the C-terminal region of the protein, in agreement with the site selectivity observed for the twoligands. Interestingly, the heterotypic complex, in which the ... as in the adaptation to variouspathological conditions, contributing to the maintenance of bile acidhomeostasis and functional distribution within the cell. Understanding the mode of binding ... high T 1⁄ T 2values in the apoprotein and in all of the investigated holo proteins,thus plays a key role in regulating the positioning of the helix–loop–helix motif with respect to the b-barrelin...
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