 a dna repair enzyme and anti inflammation chemical knockout analysis for dna polymerase  using curcumin derivatives

Báo cáo y học: " Epithelial cell senescence impairs repair process and exacerbates inflammation after airway injury" doc

Ngày tải lên : 12/08/2014, 13:22

... contain many distal airway epithelial cells (Clara cells) that are vacuolated, swollen, and have exfoliated into the airway lumen (B-D) Time course of epithelial cell damage and airway inflammation ... p21WAF1/CIP1 (p21), phospho(Thr180/Tyr182)-p38 MAPK, polyclonal anti- phospho(Ser/Thr)-ataxia teleangiectasia mutated kinase (ATM)/ataxia teleangiectasia and Rad3-related kinase (ATR) substrate, ... Statistical analysis Data are expressed as means ± SEM Statistical analyses were performed by using the Excel X software program with the add-in software Statcel (OMS, Tokyo, Japan) Data obtained...

18
102
0

Báo cáo khoa học: Kinetic studies on endo-b-galactosidase by a novel colorimetric assay and synthesis of N -acetyllactosamine-repeating oligosaccharide b-glycosides using its transglycosylation activity pptx

Ngày tải lên : 31/03/2014, 07:20

... UDP-GlcNAc and UDP-Gal were kind gifts from Yamasa Corporation (Choshi, Japan) All other chemicals were obtained from commercial sources Enzyme assay b-D-Galactosidase, b-D-glucosidase and b-NAHase activities ... gradually decreased in a subsequent reaction and ﬁnally caused complete degradation (Fig 1B,C) indicating that and 10 are fairly good substrates for endo-b-galactosidase and reﬂect an Nacetyllactosamine-repeating ... substrate linkages of blood group A and B antigens, Gala1-4Galb14GlcNAc and GlcNAca1-4Galb1-4GalNAc, and GlcAb13Galb1-3Gal structures, respectively The structure of the site of cleavage by the enzyme, ...

11
365
0

Báo cáo hóa học: "Research Article A Joint Time-Frequency and Matrix Decomposition Feature Extraction Methodology for Pathological Voice Classiﬁcation" doc

Ngày tải lên : 21/06/2014, 19:20

... Berry, M Browne, A N Langville, V P Pauca, and R J Plemmons, “Algorithms and applications for approximate nonnegative matrix factorization,” Computational Statistics and Data Analysis, vol 52, ... Indian Institute of Technology, Kharagpur, India, 2004 [6] A A Dibazar, S Narayanan, and T W Berger, “Feature analysis for automatic detection of pathological speech,” in Proceedings of Annual ... essential for feature analysis and pattern classiﬁcation In this paper, we presented a joint TF and MD feature extraction approach for pathological voice classiﬁcation The proposed methodology extracts...

11
223
0

High depth resolution rutherford backscattering spectrometry with a magnet spectrometer implementation and application to thin film analysis 2

Ngày tải lên : 14/09/2015, 08:44

... _ (a) (b) Fig 3.5 (a) The steerer table and (b) the 90° analyzing magnet on the analyzer table Source: [33] On both steerer and analyzer tables, a beam profile monitor (BPM) and a faraday cup ... buildup and tank sparks Other forms of electrical insulation are the vacuum and glass insulation within the accelerator tube, the plastic support members in the power supply and a variety of insulating ... probe, and the resultant ion beam is focused by applied voltage at the extraction electrode A set of permanent magnets apply an axial magnet field to create a toroidal motion of electrons that concentrates...

6
171
0

High depth resolution rutherford backscattering spectrometry with a magnet spectrometer implementation and application to thin film analysis 3

Ngày tải lên : 14/09/2015, 08:44

... goniometer attachment in the main chamber from a load-lock chamber through a gate valve A controller program at the control cabinet oversees the vacuum interlocks system and allows for programmed or manual ... load lock chamber (a) (b) Goniometer Gate Valve V1 V1 Main Chamber Load lock chamber (c) Main chamber Main viewport Fig 3.7 (a) Main chamber, goniometer and load lock (b) Load lock with a sample ... orientation within the main chamber It allows for translation in the x, y θ Motor and z axes, as well as rotation about the θ and φ axes (Fig 3.9) The translation resolution is 0.01 mm with a repeatability...

10
171
0

High depth resolution rutherford backscattering spectrometry with a magnet spectrometer implementation and application to thin film analysis 4

Ngày tải lên : 14/09/2015, 08:44

... functions using Mathematica, and ε was calculated using the measured value of B A graph of surface edge position vs ε was plotted and compared to the numerical and analytical calculated data using Mathematica ... ∆p at a small angle θ and displacement x0 relative to the central trajectory will exit the magnet at an angle θ and displacement x with its momentum unchanged in magnitude A matrix approach was ... Numerical and analytical (Direct and Matrix) calculations Numerical calculations show slightly better agreement to the experimental than the analytical calculations, due to more accurate modeling...

89
392
0

High depth resolution rutherford backscattering spectrometry with a magnet spectrometer implementation and application to thin film analysis 1

Ngày tải lên : 14/09/2015, 08:44

... SiOxNy and Hf-based films will have to be used Lanthanide (Rare-Earth)-based films are one of the candidates for high-k replacements as they have large band gaps, high dielectric constants and low ... endstation in CIBA are described in detail in chapter The spectrometer ion optics was simulated using numerical and analytical calculations and the results are compared to experimental data in chapter ... candidates [2 − 4], including dielectrics based on Si, Al, Hf, Zr, Ti, Y, Ta and Lanthanides In fact, Intel has fabricated a functional CMOS gate stack with a SiOxNy gate dielectric as early as...

50
244
0

Báo cáo khoa học: Plant DNA polymerase k, a DNA repair enzyme that functions in plant meristematic and meiotic tissues docx

Ngày tải lên : 07/03/2014, 15:20

... 5¢-AGCTACCATGCCT GCACGAAGAGTGCGTATTATGCCTACACTGGA GTACCGGAGCATCGTCGTGACTGGGAAAAC-3¢ [3H]dTTP (10 lM; 10 CiÆmmol)1) and enzymes were added as indicated in the ﬁgure legends, and incubated for ... dNTP was analyzed by M urea and 10% PAGE and autoradiography (B) TdT activity was measured under the conditions described in Materials and methods Commercial TdT (Takara) was assayed as a positive ... S., Kasai, N., Shimazaki, N., Takemura, M., Asahara, H., Linn, S., Yshida, S., Matsukage, A. , Koiwai, O., Sugawara, F., Yoshida, H & Sakaguchi, K (2002) A plant phytotoxin, solanapyrone A, is an...

9
492
0

Báo cáo Y học: A novel DNA repair enzyme containing RNA recognition, G-patch and speciﬁc splicing factor 45-like motifs in the protozoan parasite Toxoplasma gondii potx

Ngày tải lên : 08/03/2014, 22:20

... 5¢-CTTCACCTGGAGGAGATTTCC AAA-3¢ for 5¢ RACE and N34S 5¢-GGGAGGGTC TCGGCGTCAACAAAC-3¢ for 3¢ RACE Nested PCR was performed using internal oligonucleotides S10 5¢-GT CGAGATGTTGGTTGTCGGAGACC-3¢ for 5¢ RACE and PR2AS ... searches and sequence analysis Searches within nonredundant databases were performed using PSI-BLAST [18] at NCBI The Smart and Pfam databases were searched using RPS-BLAST Hydrophobic cluster analysis ... known DNA repair enzymes could be found in the databases, suggesting that these proteins may represent a novel family of DNA repair enzymes Moreover, searches against domain databases (Pfam, Smart)...

9
421
0

Báo cáo y học: "Is closer linkage between systemic lupus erythematosus and anti-double-stranded DNA antibodies a desirable and attainable goal" ppsx

Ngày tải lên : 09/08/2014, 06:22

... anti- Sm antibody results in the fulfillment of two criteria, namely criteria 10 (anti -DNA/ anti- Sm antibody) and 11 (a positive antinuclear antibodies (ANA) test, caused by the anti -DNA/ anti- Sm antibody) ... classification and diagnosis For example, the previous presence of anti- dsDNA antibodies (by any assay) and accordingly a positive ANA test will qualify a patient with a UV-sensitive rash for SLE classification ... [19,20] The broad definition of ‘antibody to native DNA in an abnormal titer’ [4] reflects technical standards more than 30 years old and has allowed an outgrowth of methods for anti- dsDNA antibody...

3
336
0

Báo cáo y học: "Genetic polymorphisms in the nucleotide excision repair pathway and lung cancer risk: A meta-analysis"

Ngày tải lên : 31/10/2012, 14:59

... P450s All activated carcinogens can bind to DNA and form DNA adducts that are capable of inducing mutations and initiating carcinogenesis The capacity to repair DNA damage induced by activated carcinogens ... performed using STATA Version 8.2 (Stata Corporation, College Station, TX) software Results 3-1 DNA repair capacity and lung cancer risk Cigarette smoke contains several thousand chemicals that ... The XPA protein is required for NER and is involved in the DNA damage recognition process Both RPA and XPA preferentially bind damaged DNA, and because RPA and XPA directly interact in the absence...

13
711
0

Tài liệu Báo cáo khoa học: Human enhancer of rudimentary is a molecular partner of PDIP46/SKAR, a protein interacting with DNA polymerase d and S6K1 and regulating cell growth docx

Ngày tải lên : 19/02/2014, 05:20

... GCGGGATCCCTCAGCCCATTGGAAGGCACC ATAAGAATGCGGCCGCTCAGCTGTCACTCAGCCGCAGCAG GCGGGATCCAACAAGGAAGAACCCCCC ATAAGAATGCGGCCGCTCAGTCTGAGGTGATAACATTCCC GCGGGATCCCTGGACGGGCAGCCGATGAAG ATAAGAATGCGGCCGCTCAAAGCTTGATTTTGAATTCTGT ... GCGGGATCCAACAAGGAAGAACCCCCC ATAAGAATGCGGCCGCTCAAGGCAGCTCGCTCTCCTTTTT GCGGGATCCCTCAGCCCATTGGAAGGCACC ATAAGAATGCGGCCGCTCAAGGCAGCTCGCTCTCCTTTTT GCGGGATCCGTGAATAATCTGCACCCTCGA ATAAGAATGCGGCCGCTCAAGGCAGCTCGCTCTCCTTTTT ... AAACTGCAGGATGGCGGACATCTCCCTGGAC AAACTGCAGAAGCTTGATTTTGAATTCTGT AAACTGCAGGATGGCGGACATCTCCCTGGAC AAACTGCAGAAGCTTGATTTTGAATTCTGT GCGAAGCTTCACGATGCCCAAGAAGAAGCCGACGCC GCGGGATCCCGGATGCTGGCAGCGTGGGTTGG...

14
517
0

Tài liệu Báo cáo khoa học: Specific targeting of a DNA-alkylating reagent to mitochondria Synthesis and characterization of [4-((11aS)-7-methoxy-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4]benzodiazepin-5-on-8-oxy)butyl]-triphenylphosphonium iodide doc

Ngày tải lên : 20/02/2014, 11:20

... mitoDC-81 after which time mtDNA was isolated and cut with ClaI All DNA samples were separated on an agarose/ethidium bromide gel and the ﬂuorescence recorded (A and C) The DNA was then transferred ... with ClaI (C and D) DNA was separated by electrophoresis on an agarose gel and the ethidium bromide ﬂuorescence recorded to quantify DNA (A and C) The DNA was then electrotransferred to a nylon ... EDTA and lg was run at 60 V on a 0.6% agarose/ethidium bromide gel The DNA was electrotransferred to a positive nylon membrane (Hybond N+, Amersham Pharmacia Biotech), treated with 0.4 M NaOH, and...

10
638
0

Tài liệu Báo cáo khoa học: "A SPEECH-FIRST MODEL FOR REPAIR DETECTION AND CORRECTION" docx

Ngày tải lên : 20/02/2014, 21:20

... text analysis Results reported here are for prediction on only TI repair utterances 6We performed the same analysis for the last and first syllables in the reparandum and repair, respectively, and ... glottalization is usually associated with fragments, not all fragments are glottalized In our database, 62% of fragments are not glottalized, and 9% of glottalized reparanda offsets are not fragments ... into a reparandum interval, a disfluency interval, and a repair interval We have presented empirical results from acoustic-phonetic and prosodic analysis of a corpus of repairs in spontaneous...

8
502
0

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulﬁde reductase from methanogenic archaea pdf

Ngày tải lên : 21/02/2014, 03:20

... arrow indicates the absorption maximum of the a band at 557 nm absorption maxima at 420 nm (c band), 530 nm (b band) and 557 nm (a band) are characteristic of cytochrome b Heme was extracted from ... of AF499 was identiﬁed as an archaeal promoter element by sequence analysis The sequence AAAGGTTAATATA shows a high level of identity with the consensus sequence ()35 to )23, AAANNNTTATATA); ... enzyme At potentials higher than mV, an unusual paramagnetic species was detected with g values at 2.031, 1.994, and 1.951 The resonance started to develop at potentials ‡ mV and was stable at...

10
564
0

Individual Preparedness and Response to Chemical, Radiological, Nuclear, and Biological Terrorist Attacks - A Quick Guide potx

Ngày tải lên : 15/03/2014, 15:20

... study was conducted within RAND’s Public Safety and Justice program RAND Public Safety and Justice conducts research and analysis that helps inform policymakers and communities in the areas of ... particulate filter, which will protect against radiological dust and fallout, as well as biological agents These are inexpensive, readily available, and easy to store at home, at work, and in the car ... eyes and skin Terrorists could use any of numerous chemical agents in an attack, including both industrial chemicals and chemical warfare agents Chemical weapons act very quickly, often within a...

35
326
0

Báo cáo khoa học: The sulfur atoms of the substrate CoA and the catalytic cysteine are required for a productive mode of substrate binding in bacterial biosynthetic thiolase, a thioester-dependent enzyme doc

Ngày tải lên : 16/03/2014, 04:20

... Merilainen et al ¨ Table Calorimetric analysis of CoA binding to the Z ramigera biosynthetic thiolase at 25 °C The values and error estimates are calculated from separate measurements (three for ... Science: A Guide to Enzyme Catalysis and Protein Folding WH Freeman and Co., New York, NY 34 Haapalainen AM, Merilainen G, Pirila PL, Kondo N, ¨ ¨ Fukao T & Wierenga RK (2007) Crystallographic and ... AcAc-SPP [3]; for example, Km and kcat are 73 lm and 469 ⁄ s for AcAc-SPP, whereas they are 24 lm and 465 ⁄ s, respectively, for AcAc-CoA In the crystal structure, the binding modes of the reactive...

13
472
0

Báo cáo khoa học: Initiation of JC virus DNA replication in vitro by human and mouse DNA polymerase a-primase ppt

Ngày tải lên : 17/03/2014, 03:20

... beforehand with a primase assay on poly (dT) Lanes and 2, control reaction with DNA polymerase a- primase lacking TAg or vice versa; lanes and 4, 0.2 U and 0.4 U of human; lanes and 6, 0.2 U and ... according to Bradford [41] using a commercial reagent with BSA as a standard (Biorad, Munich) DNA polymerase a and DNA primase assays were performed as previously described [34,42,43] Preparation of ... mgÆmL)1 heat treated BSA, and lCi [a- 32P]dCTP and lCi [a- 32P]dTTP (each 3000 CiÆmmol)1, AmershamBiosciences) Recombinant DNA polymerase a- primase was added as indicated After incubation for 90 at 37...

8
326
0

Báo cáo Y học: A b-lysine adenylating enzyme and a b-lysine binding protein involved in poly b-lysine chain assembly in nourseothricin synthesis in Streptomyces noursei pot

Ngày tải lên : 17/03/2014, 17:20

... the bacterial cell walls are formed by amino-acid ligases such as UDP-N-acetyl-muramoyl-L-alanine-glutamate ligase, glutathione synthetase or D-Ala-D-Ala ligase [37] They condense carboxylate-containing ... primers for PCR The pair pcp 15, GAG CACGGCMGRGAGGAGGC/PCP; 6, SGCSARGTG SCCSACSGT gave a clone encoding a partial sequence of NpsB DNA manipulations All DNA manipulations were performed according ... amino-acid recognition and their activation as an aminoacyl adenylate, and the peptidyl carrier domain (PCP-domain or T-domain) C-terminal to the A- domain providing a covalently bound 4¢-phosphopanthetheine...

11
559
0

Báo cáo khoa học: Brassinin oxidase, a fungal detoxifying enzyme to overcome a plant defense – puriﬁcation, characterization and inhibition potx

Ngày tải lên : 30/03/2014, 04:20

... experimental procedures Chemicals and deglycosylating enzymes were purchased from Sigma-Aldrich (Oakville, Canada) and chromatography media and buffers from GE Healthcare (Quebec, Canada) HPLC analysis ... [46] using the Sigma prepared reagent and BSA as the standard Synthesis and spectroscopic characterization of phytoalexins and analogue library Compounds and phytoalexins shown in Tables and supplementary ... 137 89 QSSASTMR KALAAFAADRA RLAAAFAVSRM RAVFPSIVGRS AYPGYAPFR RGYSFTTTAERE RNTLLIAGLQARN MLLLSQPGRAR TLYGGMLDDDGR KDQLLLGPTYATPKV RLEGLTDEINFLRQ LAAPVAVVTGASRGIGR KHSGPNSADSANDGFVRL RGMGGAFVLVLYDEIKKF...

15
437
0