... by presenting the pulse of Src activity in the plane ofthe inactive and active fractions, si and s1 If the duration ofthe stimulus exceeds the critical value, the trajectory in the (si, s1) ... states ofthe Src cycle When there are three intersections, the steady state O1 at the lower branch ofthe Z-shaped curve (i.e low Src activity) and the state O3 at the upper branch (i.e high Src activity) ... Kholodenko fractions during a switch-like transition from the Off state to On state (in the bistability domain) Regardless ofthe slope, the active Src fractions increase during the Off to On transition,...
... wild-type ALP I On the other hand, the Tm of D266N/Y269A-ALP I increased by only °C The denaturation temperature indicates the stability ofthe structure ofthe protein The difference ofthe increments ... surrounding residues ofthe depression, and the side chain ofthe mutated residue might restrain the mobility ofthe N-terminal region on the surface by interaction with the main chain of Lys26 by van ... ofthe original activity after h The activity of N263D-Sendai decreased to 45% and 10% ofthe original after and h of incubation at pH 12, respectively N263D/A266Y-Sendai showed only 10% of the...
... locks the enzyme in the active form for CTP synthesis and thereby stimulates the kcat ofthe reaction Since GTP actually will activate the uncoupled glutaminase reaction the structure ofthe monomer ... kinetics of GTP activation ofthe glutaminase half-reaction differed markedly on whether the reaction was coupled to CTP synthesis or not (Fig 4A) The glutaminase activity in the absence of GTP, ... equilibrium between the inactive and active form ofthe monomer with respect to CTP synthesis that involves rearrangements ofthe monomer that brings together the glutaminase site and the active site...
... the presence of these ABD constructs, the concentrations of fluorescent actin polymers appeared to be higher because ofthe precipitation of crosslinked actin polymer networks onto the glass surface ... F-actin, but also induced a shift of actin from the pellet (F-actin) to the supernatant (G-actin; Fig 3C) fraction This cosedimentation activity was also detected for residues 1–154 and 1–305 of ... 221–305 of spinophilin (not shown) A shift of F-actin from the pellet to the supernatant fraction may be explained by either sequestration of actin monomers FEBS Journal 275 (2008) 59–68 ª 2007 The...
... happen Find them The group were able to get to the British fort because of bad weather The French discovered them but it was too late to stop them and the daughters met again with their father Hawkeye ... about it The Indians took the horses away and the two groups met again near a river They then went and hid in a cave which was by the side ofthe river They heard terrible screams in the night ... middle ofthe river It was dark when Hawkeye woke Duncan The younger ofthe sisters let a cry It seemed though the Hurons filled the woods It is the scream of a horse great pain The noise...
... may act as a medium in the catalytic reaction The position of Lys78 is anchored with an interaction between the main chain oxygen of Ala216 and the e-amine of Lys78 In H34A mutant (Fig 6B), the ... occur [10] However, further characterization ofthe D214A mutant was not conducted because of difficulties in removing PMII from the activation sample Removal of PMII from the sample, despite being ... orientation of active residues and water molecules In the energy-minimized models of H34A (Fig 6b), the orientations of Asp214 and Water2 are altered in a way that increases the distance between the...
... that the ATPase activity ofthe NBDs is influenced both by the presence ofthe transmembrane domains of SUR1 and by the tetrameric Kir6.2 pore Thus, just as SUR1 influences the channel activity of ... time period ofthe experiment The rate ofthe reaction was proportional to the amount of protein used, and there was a linear relationship between protein concentration and activity The protein ... ATPase activity by about 60% If we assume that the relative extent of ATPase activity at NBD1 and NBD2 remains the same in full-length SUR1 (i.e that of NBD1 is 20% of that of NBD2), then the...
... (lane 8) were also detected in the nuclear fractions The significance ofthe presence of these minor forms of ZF-RNases in the nucleus was not yet clear However, these results clearly demonstrate ... calculating the standard deviation ofthe DCt (i.e Ct ofthe target gene – Ct ofthe reference gene) [68] Cleavage of rRNA precursor Two substrates, both containing the A0 cleavage site of rRNA precursor, ... understanding the role of ANG in motor neuron physiology and in the therapy of motor neuron diseases [60] ANG gene knockout in a mouse model might be complicated because ofthe existence of six isoforms...
... mg of purified CT-peptide per liter of bacterial culture Effect ofthe CT-peptide on mPC1 ⁄ enzymatic activity The effect of various concentrations ofthe purified CT-peptide on the cleavage ofthe ... expressing the recombinant mPC1 ⁄ 3, the presence ofthe 87 kDa form in excess ofthe 66 ⁄ 71 kDa facilitates isolation ofthe enzyme and helps in maintaining the enzymatic activity at a proper level The ... removal ofthe CT-peptide to release the fully active PC1 ⁄ within the confines ofthe secretory granules [35] We decided to investigate whether the propeptide and the CT-peptide can act synergistically...
... defined term in the Securities Act, and other rules under the Securities Act, such as Rule 482, specifically refer to an “investment company registered under the Investment Company Actof 1940.” Rule ... Predictions of performance, exaggerated claims of investment prowess, unwarranted comparisons to competitors or benchmarks, and all ofthe other practices listed in the rule carry the same potential ... net, presumably seeking to attract the greatest number of investors they can that colorably meet the definition of accredited investor Many, and perhaps most, of these investors are likely to...
... characteristic ofthe monoformazan, i.e the fully reduced product of INT [15,17] In the following aeration ofthe medium, the absorbance ofthe spectral bands decreased, but the shape ofthe ... [13], either by measurement ofthe rate of production of O2– or the rate of O2 uptake INT diaphorase activity was assayed by the rate of reduction of INT into formazan in the presence of superoxide ... Addition of benzylimidazole to hemin resulted in the decrease ofthe high spin signal at g ¼ 6.0 (Fig 4, trace d), characteristic ofthe pentacoordinated form ofthe iron atom ofthe heme, and in the...
... EQ-5D items the frequency of each possible response was computed to identify whether responses were distributed across the range of possible scores or whether they were at either end ofthe scales ... A test-retest reliability study ofthe Barthel Index, the Rivermead Mobility Index, the Nottingham Extended Activities of Daily Living Scale and the Frenchay Activities Index in stroke patients ... cater for the needs of older adults Evaluation of these services and ongoing monitoring of their patients requires that participation in functional activities and health-related quality of life...
... According to the actometer measures, 29% ofthe patient population had a passive activity pattern and 71% had a fluctuating active one Validity ofthe instruments The correlation ofthe API and the IPAQ ... these topics the therapist forms a final judgement in the form of a dichotomous outcome, namely 'passive' or 'active' These topics are: the routine pattern of activities and the amount of time laying ... table show the area under the curve (representing the validity) for the CFS-AQ, the IPAQ and the API As can be seen, the validity ofthe API (0.643) was somewhat smaller than that ofthe two questionnaires...
... cycle was the onset of displacement ofthe marker on the head ofthe third metacarpal and the end ofthe cycle was the return ofthe marker to the starting point after completing the drinking task ... In the arm, the origin ofthe reference system was at the center ofthe glenohumeral joint, cm below the acromion Also, the Y-axis corresponded to the line that joined the midpoint between the ... sequence of ZXY rotations ofthe trunk, arm and hand, and ZYX rotations ofthe forearm In each recording, a complete cycle ofthe drinking task was identified The beginning ofthe cycle was the onset...
... from the bottom to the top ofthe tree crown Like in the PAR domain, the variability ofthe transmittance was more marked at the top and the bottom than in middle part ofthe tree crown The difference ... nm–1) The action spectra ofthe two forms of phytochrome overlap throughout the 400–800 nm waveband The parameter describing the incident active radiation on the phytochrome is primarily the phytochrome ... is the length ofthe beam path in cell l Leaf dispersion is assumed to be random The two terms ofthe right member of Equation (1) respectively account 499 Spatial distribution of MAR for i) the...
... different terms ofthe radiation balance in the PAR above and below the canopy for clear weather (day 193) as a function ofthe hour ofthe day The transmission ofthe incident PAR varies with the solar ... stopped the direct PAR The error induced on the measurement was small: to account for the effect ofthe part ofthe sky vault hidden by the shadow band, a multiplier of 1.084 given by the manufacturer ... the canopy and the understorey is presented in figure The increase in the canopy PAR reflectance at the beginning and at the end ofthe day is due to the interception ofthe top ofthe plant canopy...
... drafting ofthe manuscript JL participated in the planning ofthe study and in the drafting ofthe manuscript TP participated in the planning ofthe study, in the analysis of data, and in the drafting ... around the transition into DST in 2003 Graphics consist of four panels The first panel from the top shows the original raw data, the second the circadian effect, the third the trend effect and the ... mesor (fitted mean), the acrophase (time ofthe peak ofthe fitted curve) and the amplitude (magnitude ofthe oscillation) were determined To calculate these three variables, the raw data originally...
... equivalence ofthe factor loadings (i.e., metric invariance), intercepts (scalar invariance), and then residual variances (strict invariance) We set the metric ofthe scale by constraining the factor ... change the fit indices (see Table for factor loadings and residuals) Together, these findings imply that there are no threats to longitudinal invariance for the revised version ofthe PACES, and the ... interpretations of enjoyment scores Little is known about the development of physical activity enjoyment among older adults Within the interactionist framework of social cognitive theory (SCT) [8,...
... ribonuclease activity Impact of athe skin surfaceat the 5'end ofthe substrate Impact of a Cap structure at the 5'end ofthe substrate mRNA on the skin surface ribonuclease activity Ribonuclease activity ... TLR-8.[15-18] The characterization ofthe extracellular ribonuclease activity has become again an attractive topic at the postgenomic era, where the development of safe gene therapies is needed for the ... such activities at their surface All three hypothesized roles of these ribonucleases on the skin (protection against foreign pathogens like RNA-viruses, prevention ofthe activation ofthe immune...
... culture model of hyperglycemia Thereby, baicalin was identified as the primary bioactive compound in the herbal extract The antioxidant potential of baicalin was further characterized in the STZ-induced ... repeated the work of Von Mering and Minkowski but went a step further to show the reversal of diabetic symptoms in the dogs by giving them an extract from the pancreatic islets of Langerhans of healthy ... Huey Lee Then, the author wishes to express her gratitude to Professor Bay Boon Huat ofthe Department of Anatomy, Yong Loo Lin School of Medicine and Associate Professor Heng Chew Kiat ofthe Department...