—detection of blood stains etc

Báo cáo hóa học: " cDNA targets improve whole blood gene expression profiling and enhance detection of pharmocodynamic biomarkers: a quantitative platform analysis" ppt

Ngày tải lên : 18/06/2014, 16:20

... Functional identity of genes detectable in expression profiling assays following globin mRNA reduction of peripheral blood samples Clin Biochem 2007, 40:499-502 Affymetrix: An Analysis of Blood Processing ... profiling, there are a number of technical challenges The first is mRNA stabilization and isolation The introduction of point -of- collection products that stabilize nucleic acids for whole blood ... delays in processing blood samples can lead to changes in expression of thousands of genes [9,12,13] Another challenge is that the specificity and sensitivity of a given RNA profiling platform are...

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Báo cáo hóa học: "Detection of carcinoembryonic antigen messenger RNA in blood using quantitative real-time reverse transcriptase-polymerase chain reaction to predict recurrence of gastric adenocarcinoma" potx

Ngày tải lên : 18/06/2014, 16:20

... Center of Sun Yat-Sen University The study was undertaken in accordance with the ethical standards of the World Medical Association Declaration of Helsinki Pre-processing of blood samples Blood ... morphology of circulating cancer cells and found that the majority of circulating breast cancer cells was in a state of apoptosis In the peripheral blood of cancer Figure Disease-free survival of patients ... cycle of 4), folfox6 (56 cases, with a median cycle of 6), taxol + cisplatin (4 cases, with a median cycle of 4), taxol + 5FU/ Page of CF (Fluorouracil/Leucovorin, cases, with a median cycle of...

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Báo cáo sinh học: "Concomitant detection of IFNa signature and activated monocyte/dendritic cell precursors in the peripheral blood of IFNa-treated subjects at early times after repeated local cytokine treatments" doc

Ngày tải lên : 18/06/2014, 19:20

... for 24 hours to 1,000 U/ml of IFNa2b (Intron A) Cytofluorimetric analysis of monocytes ex vivo Statistical analyses For cytofluorimetric analysis, 30 ml of peripheral blood were collected into ... of 15 of the cytokine (T0 vs T0+24 or T1m vs T1m+24, Figure 2C-D) The same pattern of segregation was obtained for donors receiving 1MU of IFNa (data not shown) Similarity of the modulation of ... expression between two doses of IFNa tested Taking advantage of the availability of blood samples obtained from patients receiving two different doses of IFNa, in the contest of the HBV study, we investigated...

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báo cáo khoa học: "Detection of DNA mismatch repair proteins in fresh human blood lymphocytes - towards a novel method for hereditary non-polyposis colorectal cancer (Lynch syndrome) screening" doc

Ngày tải lên : 10/08/2014, 10:21

... 10 ug of total cell protein (C) Detection of MLH1 and MSH2 proteins on western blots with a mixture of varying amounts of HCT116 and LoVo cell lysates Results show that the combinations of these ... Page of Figure Expression of MLH1 and MSH2 proteins in fresh blood and in immortalized lymphocytes following PHA stimulation (A) Timedependent stimulation of MLH1 and MSH2 proteins in fresh blood ... F Density of the bands of interest on a western blot was determined by scanning of the x-ray film and highlighting the band area using a BioRad Gel 2000 documentation system and its software The...

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báo cáo khoa học: "Low-level expression of HER2 and CK19 in normal peripheral blood mononuclear cells: relevance for detection of circulating tumor cells" doc

Ngày tải lên : 10/08/2014, 22:20

... Understanding the biology of the background expression of tumor markers will be instrumental in development of more specific methods to detect CTC Isolation of PBMC from Whole Blood Blood was collected ... blood of normal controls, and that the HER2 signal was frequently present in the peripheral blood of both normal controls and breast cancer patients In addition, the HER2 signal seen in the blood ... sensitivity of HER2 detection in blood, real time quantitative RTPCR was performed on RNA isolated from mock positive controls of ml of leukopak cells spiked with serial dilutions of breast cancer...

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Báo cáo y học: "Comparison of a Two-Lead, Computerized, Resting ECG Signal Analysis Device, the MultiFunction-CardioGramsm or MCG (a.k.a. 3DMP), to Quantitative Coronary Angiography for the Detection of Relevant Coronary Artery Stenosis (70%)

Ngày tải lên : 03/11/2012, 10:58

... large inventory of normalized mathematical indexes of abnormality It is the pattern of these mathematical indexes of abnormality, obtained from analysis of multiple cardiac cycles of the resting ... assess the performance of the prediction of stenosis independent of the prevalence of stenosis, the positive and negative likelihood ratios (LR) were calculated [24] A value of p

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Genotoxicity of 255 chemicals in the Salmonella microsome test (Ames test) and 8-hydroxyguanine (8-OH-Gua) assay for the detection of carcinogens

Ngày tải lên : 05/09/2013, 08:40

... chemicals These strains were kindly supplied by Dr T Nohmi of the National Institute of Health Sciences S9 fraction of phenobarbital/5,6-benzoflabone-pretreated male Sprague-Dawley (SD) rat liver ... We compared the mutagenic activity (shown as the number of revertants per nanomolecule of chemicals, rev./nmol) of YG strains with that of the conventional TA strains as shown in Fig YG1041 showed ... Identification of C8-modified deoxyinosine and N2- and C8-modified deoxyguanosine as major products of the in vivo reaction of N-hydroxy-6-aminochrysene with DNA and the formation of the adducts...

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Detection of bacteria and enteric viruses from river and estuarine sediment

Ngày tải lên : 05/09/2013, 10:15

... and visualized by UV illumination Evaluation of recovery rate of preparation method for viruses in sediment As a surrogate of RNA viruses, Sabin strain of Poliovirus type was spiked into the sediment ... capsid of approximately 25 nm diameter This virus was also used by Gerba et al (1977) for evaluation of the method Recovery rate was evaluated by dividing amount of spiked Poliovirus by amount of ... procedure of Craig et al (2002) with some modifications: mL double-distilled water was used instead of mL 0.1% peptone water; a vortex mixer was used for 15 sec instead of hand - 310 - Journal of Water...

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Detection of Antiviral Drugs Oseltamivir Phosphate and Oseltamivir Carboxylate in Neya River, Osaka, Japan

Ngày tải lên : 05/09/2013, 10:15

... periods may result to the development of oseltamivir-resistant influenza viruses Furthermore, Japan is at the top of the list of countries for per capita consumption of Oseltamivir (Soderstrom et al., ... ST-2 from the 34th week of 2009 to the 7th week of 2010 period were obtained from Osaka Prefectural Institute of Public Health (2010) An index value gives the total number of influenza cases (average) ... Japan Stock solutions of OP and OC (1.0 mg/L each) were prepared in methanol and stored in refrigerator (4ºC) prior to usage Description of Sampling Sites Fig shows the location of the sampling sites...

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A RUSH OF BLOOD TO THE HEAD

Ngày tải lên : 17/10/2013, 18:20

... it, seven years of bad luck Of course, if you ask us, most of us will say no, don’t be ridiculous, I give absolutely no credence to any of those inane superstitions Yet most of us continue to ... in Applied Neuroimaging at the University of Warwick, En gland, and is the founder of Neurosense in Oxford, and Professor Richard Silberstein, the CEO of Neuro-Insight in Australia And the results? ... symptoms of schizophrenia, boost the efficacy of brain cells, and improve their performance at work, over 300 million people,1 including 60 percent of all male doctors, smoke With annual sales of 1.8...

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Detection of Abrupt Changes:Theory and Application

Ngày tải lên : 26/10/2013, 17:15

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Tài liệu FUZZY CLUSTERING ALGORITHMS ON LANDSAT IMAGES FOR DETECTION OF WASTE AREAS: A COMPARISON pdf

Ngày tải lên : 16/01/2014, 16:33

... combination of bands 5, and which is of great eﬃcacy for the aims of our analysis In Figures and the set of bands 5, and are depicted respectively for the month of May 1994 and March 1997 The fusion of ... the membership values of all samples in all clusters; • m ∈ (1, ∞) is a control parameter of fuzziness The minimization of Jm , under the probabilistic constraint iteration of the following formulas: ... number of clusters, the Deterministic Annealing algorithm can start with an over-dimensioned number of clusters At high temperatures, all centers collapse to a unique point (the center of mass of...

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Tài liệu Non-blood medical care in gynecologic oncology: a review and update of blood conservation management schemes docx

Ngày tải lên : 13/02/2014, 06:20

... postoperative monitoring of the patient, early recognition of blood loss [39,47], minimisation of phlebotomy blood sampling [39], enhancement of hemopoiesis [45], optimisation of cardiopulmonary status ... which the same technique of cell savage was used [31] He reinfused 400 ml of salvaged blood into a 58-year-old woman operated for ovarian adenocarcinoma and 170 ml of salvaged blood into a 49-year-old ... [37] Preoperative autologous blood donation is another alternative This actually involves the donation of units of whole blood preoperatively over a 4-week period; the blood is then stored and given...

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Tài liệu Báo cáo khoa học: PCR detection of nearly any dengue virus strain using a highly sensitive primer ‘cocktail’ ppt

Ngày tải lên : 14/02/2014, 19:20

... either 100 pg of DENV-1-, ng of DENV-2-, 100 pg of DENV3- or ng of DENV-4-infected C6 ⁄ 36 cells total RNA was used for cocktail PCR The primer cocktail was composed of 100 nm each of 1G1P1, 1G3P6, ... strains of Japanese encephalitis virus, 28 strains of St Louis encephalitis virus, 172 strains of West Nile virus and 24 strains of yellow fever virus using BLASTn [22] The genome sequences of the ... Effect of geographical variation in dengue virus on the performance of the primer cocktail Each of the four serotypes of dengue virus can be classiﬁed into several genotypes, deﬁned as a group of...

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Tài liệu PROTEOMIC APPLICATIONS FOR THE EARLY DETECTION OF CANCER doc

Ngày tải lên : 15/02/2014, 05:20

... 70% (107 of 153) of sera from patients with benign disease and PSA levels of >4 ng/ml, and could accurately predict the presence of cancer in 95% of the patients tested, including 18 of 21 men ... the development of variations of the traditional 2D-gel approach, and the application of these has resulted in the identification of potential new biomarkers for early detection of disease Differential ... urine have proven to be a rich source of biomarkers for the early detection of cancer The blood proteome changes constantly as a consequence of the perfusion of the diseased organ adding, subtracting...

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Tài liệu Detection of Pulmonary tuberculosis: comparing MR imaging with HRCT pptx

Ngày tải lên : 15/02/2014, 12:20

... total of six zones per patient The upper zones were defined as areas of the lung above the level of the carina; the middle zones as areas between the level of the carina and the origin of the ... identified by MRI The lack of histopathological correlation or microbiological tests of the adenopathies is another important limitation of the study, and MRI in the phase of expiration may show ... radiologists (VS, EBR, both with 10 years of experience in clinical MR imaging and 25 years of experience in chest imaging) The observers were unaware of CT results to avoid interpretation bias...

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Tài liệu BONDS OF BLOOD AND HONOUR: A DUAL-STATTED INTRODUCTORY ADVENTURE FOR A GAME OF THRONES RPG WRITTEN BY JASON DURALL pptx

Ngày tải lên : 15/02/2014, 13:20

... during the Age of the First Men, or so it is said Blood of the First Men runs through the veins of many of Locke House Holdings Oldcastle is located near the terminus of the west fork of the White ... Men, or so it is said Blood of the First Men runs through the veins of some still Oldcastle is located near the terminus of the west fork of the White River, deep in the heart of the wolfswood between ... Traits Feats: Able Body, Blood of the First Men, Pious, Stubborn, Keys of Oldcastle Social Status: Rank (Member of a Minor House) [Level Organisational Ties] personalities of House Locke This section...

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Tài liệu Báo cáo khoa học: Extended half-life upon binding of destabilized intrabodies allows speciﬁc detection of antigen in mammalian cells pdf

Ngày tải lên : 19/02/2014, 18:20

... ampliﬁcation of the 3¢ end of the b-gal gene The presence of similar amounts of genomic DNA in the reactions was controlled by concomitant addition of primers that amplify a 200 bp region of the RNA ... isolation by FACS of living cells expressing low levels of antigen Discussion The potential of scFvs for demonstrating the presence of targeted protein antigens in the cytoplasm of mammalian cells ... intracellular forms of antigen To analyze how these scFvs linked to EGFP or d1-EGFP behave in case of low and constant expression of b-gal, we took advantage of the availability of two cell lines,...

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Tài liệu Báo cáo khoa học: "Automatic Extraction of Lexico-Syntactic Patterns for Detection of Negation and Speculation Scopes" pdf

Ngày tải lên : 20/02/2014, 04:20

... Statistics of the BioScope corpus The 2nd and 3d columns show the total number of cues within the datasets; the 4th and 5th columns show the percentage of negated and speculative sentences 70% of the ... children (starting from the root of the subtree) to the rule pattern subtree Nodes of type *scope* and * match any number of nodes, similar to the semantics of Regex Kleene star (*) Results As ... the rule tree; neighboring identical siblings of type *scope* or * are replaced by a single node of the corresponding type Figure shows an example of this transformation The rule tree pruning...

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