ảnh hưởng của các chất gây ô nhiễm trong nước thải
Commissural axon pathfinding at intermediate targets in the zebrafish forebrain
... telencephalon, the HuC line showed additional strong expression in the olfactory bulb (Figures 2-3C and 2-3D) Also, the stria medullaris was more strongly labeled in the HuC line The Kaede protein ... of the anterior forebrain in HuC: kaede transgenic fish, which have strong fluorescence in olfactory bulb neurons, led to strong label only in neurons that terminated in the telencephalon; weaker ... detected in the telencephalon (D) In an HuC:Kaede 3-dpf embryo, olfactory bulb neurons fluoresce more strongly than telencephalon neurons, as seen in this optical section (E) Photoconversion of the midline...
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Identification and characterization of proteins that interact with zonula occludens proteins
... MDCK cells increases TER of MDCK cell monolayers (Balda et al., 1996; Van Itallie et al., 2001) strongly implicate a central role for proteins in TJ function However, the role of specific lipids ... shows two of the major protein complexes that occur in TJs Of the JAM-interacting proteins, Par-3 strongly suggests a role for JAM in TJ formation since Par-3 is a component of the evolutionally...
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Tài liệu Báo cáo khoa học: Arabidopsis thaliana BTB⁄ POZ-MATH proteins interact with members of the ERF⁄AP2 transcription factor family ppt
... showed that BPM2 and BPM5 were strongly expressed in all tested tissues (roots, rosette and cauline leaves, stems and flowers) (Fig 3A) Although BPM6 was also strongly expressed, its expression ... seedlings, strongest at the base of emerging lateral roots, but no expression at detectable levels in the tips of primary and budding lateral roots proBPM2:GUS lines (Fig 4B) showed strong expression ... proBPM1:GUS, strong staining was detectable in the stipules, pollen and at the base of siliques Expression in roots was detectable along the primary but not lateral roots, with strongest staining...
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Tài liệu Báo cáo khoa học: Analysis of oxidative events induced by expanded polyglutamine huntingtin exon 1 that are differentially restored by expression of heat shock proteins or treatment with an antioxidant ppt
... similar observation was made in cells treated with NAC In contrast, the caspase-like activity was strongly altered by httEx1 3086 We have already shown that the expression of httEx1polyQ polypeptides...
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Báo cáo khoa học: Esculentin-1b(1–18) – a membrane-active antimicrobial peptide that synergizes with antibiotics and modifies the expression level of a limited number of proteins in Escherichia coli doc
... shown that this unique amphibianderived peptide: (a) kills E coli via membrane perturbation; (b) strongly synergizes with erythromycin, presumably by increasing the intracellular influx of this drug, ... heating serum at 56 °C (see Experimental procedures), our findings suggest that serum components not strongly bind to Esc(1–18) and therefore not significantly affect the availability of active peptide ... molecular weight hydrophilic materials [42], were detected in peptide-treated bacteria (Table 3), with stronger reductions being seen at 16 lm Esc(1–18) Otherwise, a slight increase in OMP W expression...
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Báo cáo khoa học: Identification of three proteins that associate in vitro with the Leishmania (Leishmania) amazonensis G-rich telomeric strand pdf
... approximately 40 kDa (Fig 1E, lanes and 7) and ‡ 60 kDa (Fig 1E, lanes and 8) In this experiment, the ÔextraÕ 24 kDa protein band (Fig 1C, lanes 4–7 and Fig 1D, lanes and 7) did not appear, probably...
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Tài liệu Báo cáo khoa học: Nop53p, an essential nucleolar protein that interacts with Nop17p and Nip7p, is required for pre-rRNA processing in Saccharomyces cerevisiae pdf
... and Nop53p interacted in the two-hybrid system independently of the tag, but the interaction was stronger when Nop17p was fused to the DNA binding domain (BD-Nop17p; Fig 1) Further protein interaction ... 5¢-end of the 25S rRNA (Fig 6C), although in this case the effect of Nop53p depletion was not as strong as observed for the 18S and 5.8S rRNAs Control experiments were performed in parallel with ... indicate that Nop53p participates in the pre60S complex, affecting the processing of the 27S and more strongly the processing of the 7S prerRNA Purified His–Nop53p was also tested for binding to in vitro...
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Báo cáo khoa học: Purification of a plant nucleotide pyrophosphatase as a protein that interferes with nitrate reductase and glutamine synthetase assays pdf
... Bessman, M.J., Frick, D.N & O’Handley, S.F (1996) The MutT proteins or ÔNudixÕ hydrolases, a family of versatile, widely distributed, ÔhousecleaningÕ enzymes J Biol Chem 271, 25059– 25062 Conyers, G.B ... be performed in the presence of >1 mM NaF, or after passing through a Con-A column to remove the ÔinterferenceÕ from extracts containing high nucleotide pyrophosphatase activity Acknowledgements ... chromatography of extracts of spinach leaves [6], or cauliflower (Fig 1) In contrast to 14-3-3 proteins, the ÔinterferingÕ protein inhibited both phosphorylated and dephosphorylated NR (Fig and data not shown)...
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Báo cáo khoa học: A region within the C-terminal domain of Ure2p is shown to interact with the molecular chaperone Ssa1p by the use of cross-linkers and mass spectrometry doc
... 337– 343 fragment is neither detected unmodified, nor modified, in the 120 kDa Ure2p–Ssa1p complex strongly suggests that it is cross-linked to Ssa1p Similarly, FEBS Journal 277 (2010) 5112–5123 ª ... lysine 325 was found to be modified in Ssa1p but not in Ure2p–Ssa1p complexes These observations strongly suggest that the exposure to the solvent of lysine 339 from Ure2p and lysine 325 from Ssa1p ... (Opti-TOF; Applied Biosystems) MALDI-TOF-TOF MS and MS ⁄ MS spectra were acquired with a MALDI-TOF ⁄ TOFÔ 4800 mass spectrometer (Applied Biosystems) in the positive and reflector mode An external calibration...
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Báo cáo khoa học: Vaccination with prion peptide-displaying papillomavirus-like particles induces autoantibodies to normal prion protein that interfere with pathologic prion protein production in infected cells pptx
... were observed The aim of this study is to develop a safe and effective vaccine that generates a strong Ab response against the prion self-antigen, but avoids induction of a T-cell response, to ... de novo synthesis of pathologic PrPSc in persistently prion-infected ScN2a cells In contrast, a strong band at the expected size of PrPSc was observed in cells treated with preimmune IgG Thus, ... did not merely bind PrP, but effectively inhibited PrPSc de novo synthesis in living cells This strongly suggests a functionally relevant protective role of the induced Ab to PrP (Fig 6), indicating...
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Báo cáo Y học: The inhibitory region of troponin-I alters the ability of F-actin to interact with different segments of myosin pot
... to be important for normal muscle activity Deletion of this loop region resulted in the loss of strong binding of myosin to actin [17] while a single amino acid residue change, ArgfiGln, in this ... of interaction with myosin essential for activation of the MgATPase The results reported here strongly suggest that the binding of IP or TnI to an actin monomer produces conformational changes...
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Báo cáo Y học: Nuclear proteins that bind to metal response element a (MREa) in the Wilson disease gene promoter are Ku autoantigens and the Ku-80 subunit is necessary for basal transcription of the WD gene ppt
... and their influence on promoter activity of the MT gene, the proximal element MREa exhibiting the strongest effect In addition, it is known that function of distal MREs is dependent on MREa in proximal...
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Báo cáo Y học: Cloning and characterization of novel snake venom proteins that block smooth muscle contraction pptx
... milligrams of ablomin were obtained from g of crude venom For detailed purification procedures, see ÔExperimental proceduresÕ reticulum (SR) The specific effect of ablomin on high K+-induced contraction ... although the complete amino-acid sequence of PsTx has not yet been reported (Fig 4) These facts strongly imply that other proteins that are homologous to ablomin may possess distinct biological ... proteins, which we isolated (His60, Glu75, Glu96, and His115 in ablomin in Fig 4) These facts strongly suggest these proteins have a three-dimensional structural similarity with other CAP family...
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Báo cáo khoa học: Polyamines interact with DNA as molecular aggregates Luciano D’Agostino1 and Aldo Di Luccia doc
... starvation for up to days ) conditions that determine a strong reduction in the concentration of polyamines in the cells [17] ) caused a strong decrease in < 1000 NAP and a reduced and delayed ... Da peak increased at h (34.1%), remained the same at h and declined at 12 h The < 1000 Da peak strongly decreased at and h (18.9 and 19.8%, respectively) after which it Fig Gel permeation chromatography ... as the mobile phase (Fig 2A) Whatever the buffer used, the GPC profiles that resulted after the Ôin vitroÕ aggregation of polyamines was very similar to those obtained by analysing the nuclear...
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Báo cáo sinh học: " Endophilins interact with Moloney murine leukemia virus Gag and modulate virion production" doc
... activity (Figure 1a) Gag interacted strongly with endophilin Only fusions containing full-length Gag or the MA domain of Gag (⌬6 and MA; see Figure 1a) interacted strongly; a large fragment retaining ... amino terminus and SH3 domains, is required for the strongest interaction with Mo-MuLV Gag No single region could be identified as sufficient for strong binding Several fragments showed weak binding, ... http://jbiol.com/content/3/1/4 other Gags tested As previously reported, all Gags displayed strong interactions with themselves Endophilin also interacted strongly with itself in the yeast two-hybrid system, suggesting that...
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