γ h2ax and the recruitment of dna repair and chromatin remodeling factors
Báo cáo y học: "A general framework for quantifying the effects of DNA repair inhibitors on radiation sensitivity as a function of dose" pptx
Ngày tải lên :
13/08/2014, 16:21
... survival and (3) the relative significance of these changes The main features of the method are the inclusion of an indicator term in the model to indicate the presence of the drug and a factor ... between treated and untreated experiments, the merits of the approach were evaluated in terms of the capacity of the model to quantify and indicate the relative significance of the effects of PARP inhibition ... comparison of the parameters in the treated and untreated experiments As a consequence, quantitative assessment of the effect of modifiers of DNA repair upon four distinct components of the radiation...
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THE INFLUENCE OF THE KU80 CARBOXY-TERMINUS ON ACTIVATION OF THE DNA-DEPENDENT PROTEIN KINASE AND DNA REPAIR IS DEPENDENT ON THE STRUCTURE OF DNA COFACTORS Derek S.
Ngày tải lên :
24/08/2014, 09:33
... Woods The Influence of the Ku80 Carboxy-Terminus on Activation of the DNA- Dependent Protein Kinase and DNA Repair is Dependent on the Structure of DNA Cofactors In mammalian cells DNA double strand ... lesions the interstand crosslink repair pathway produces nicks in the strands on either side of the crosslinked bases essentially producing a DNA double strand break The resulting break is then repaired ... in which the initial radical induced on the DNA produces a single strand break The radical is then transferred to the other strand causing the second break7 Of these two possibilities the latter...
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Báo cáo khoa học: End-damage-specific proteins facilitate recruitment or stability of X-ray cross-complementing protein 1 at the sites of DNA single-strand break repair docx
Ngày tải lên :
23/03/2014, 11:20
... specific proteins for ‘cleaning’ the ends and preparing them for DNA repair, the time and mechanism of entry of these proteins and other BER partners into the repair process is unknown This mechanism ... identification of these lesions as a strand break, verification of the nature of the 3¢ end, and identification of a satisfactory pathway for repair The XRCC1 component of the XRCC1 DNA ligase IIIa ... to the next stage of repair Therefore, Pol b initiates repair of single-nucleotide gaps, PNK is required for the initiation of repair of 3¢-phosphatecontaining SSBs, and APE1 initiates repair of...
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Báo cáo Y học: Thermodynamics and kinetics of the cleavage of DNA catalyzed by bleomycin A5 A microcalorimetric study pdf
Ngày tải lên :
24/03/2014, 04:21
... Eqns (19) and (20), respectively m Thermodynamics of the binding of ADM and (1,10-phenanthroline)-copper to DNA Fig A comparison of the UV and visible spectrum of BLM-A5 after the cleavage of calf ... curves The molar ratio, r, of DNA to L is defined as r ¼ nDNA;0 =nL;0 ¼ DNA 0 =½L0 ð17Þ where nDNA,0 and nL,0 are the initial amounts of DNA and L, respectively Substituting Eqns (15) and (17) ... binds to DNA and DNA L the complex between DNA and L The intrinsic binding constant, KB, is defined by the equation [24,28,29]: KB ¼ y ð1 À yÞð DNA 0 À ny½L0 Þ ð14Þ Here, [DNA] 0 and [L]0 are the initial...
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Báo cáo khoa học: "Comparison of RBE values of high- LET a-particles for the induction of DNA-DSBs, chromosome aberrations and cell reproductive death" potx
Ngày tải lên :
09/08/2014, 09:20
... distance of 50 mm underneath the dishes and the particles passed through 50 mm of helium and the thin mylar bottom of the culture dishes before entering the cells through the bottom of the dishes ... fraction of the DSB (about 1% of DSB induced by g-rays and about 10% by a-particles) are causing cell death On the other hand, the values of a for formation of chromosome fragments and colour ... Page of that many of these aberrations are either repaired or not cause complete impairment of the cell reproductive capacity The number of fragments is higher than that of colour junctions as the...
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Báo cáo sinh học: " The effects of DNA formulation and administration route on cancer therapeutic efficacy with xenogenic EGFR DNA vaccine in a lung cancer animal model" pps
Ngày tải lên :
14/08/2014, 19:22
... The presence and the therapeutic efficacy of anti-EGFR antibody in serum from the DNA vaccine group of mice The presence and the therapeutic efficacy of anti-EGFR antibody in serum from the DNA ... naked DNA vaccine elicited Th1-bias immune response Hence, the choice of the route of DNA immunizations and formulation of DNA could thus represent an important element in the design of EGFR DNA ... with the design of the experiments and prepared the draft manuscript, MCY, CFT, CCW, PSL and HJY performed the experiments; CML provided advice on the design of the study and commented on the...
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The roles of DNA(cytosine 5) methyltransferase1 in carcinogenesis related to cellular factors, virus and chemicals
Ngày tải lên :
16/09/2015, 15:55
... involved in the maintenance of the methylation pattern during DNA replication where, the methylation of the parental strand is faithfully copied onto the daughter strand Two other families of enzymes ... 1999) They are also involved in the segregation of chromosomal DNA (Meury et al., 1995) and mismatch repair namely the mut repair system where MutH binds to hemimethylated DNA and cleaves the non-methylated ... discovered that they are involved in the longevity of Drosophila The intactness of the gene was required for the maintenance of the normal life span and over-expression resulted in prolonging the life...
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Báo cáo Y học: Kinetic studies of human tyrosyl-DNA phosphodiesterase, an enzyme in the topoisomerase I DNA repair pathway pot
Ngày tải lên :
31/03/2014, 21:21
... speculate that the release of DNA and TDP from the transition state complex might involve an activated water molecule in the active site Requirement of manganese as cofactor and the pH profile of TDP ... study, we report the development of a sensitive colorimetric assay in a 96-well format, the identification of a cofactor, and characterization of kinetic parameters of the human tyrosyl -DNA phosphodiesterase ... monitored and the amount of released products was calculated based on the extinction coefficient (e) of 15 000 M)1Æcm)1 The rate was then determined as the amount of released p-nitrophenol per The pH...
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The situation of increasing serum uric acid, related factors and efficacy of dietary intake in over 30 year of age subjects in rural area of thai binh province
Ngày tải lên :
21/10/2014, 20:53
... purine of ARN and 20% of ADN derived from food Therefore, the cases of increased serum uric acid should reduce the consumption of alcohol, soft drinks and fructose, reduce consumption of products of ... group of 70-79 and 4.5 times in the age group of 80 and older as compared to the age group of 30 Our results were consistent with epidemiological studies in Vietnam and oversea Assessment of the ... - Research and analysis of a purine content of food availability and characteristics of Vietnam MINISTRY OF EDUCATION AND TRAINING MINISTRY OF HEALTH NATIONAL INSTITUTE OF HYGIENE AND EPIDEMIOLOGY...
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Báo cáo khoa học: Death inducer obliterator protein 1 in the context of DNA regulation Sequence analyses of distant homologues point to a novel functional role docx
Ngày tải lên :
07/03/2014, 21:20
... found a set of DIDO1 homologues in the context of DNA binding and chromatin regulation These homologues include members of the CpG binding protein (CGBP) family of proteins that bind DNA at unmethylated ... from the other branches, which was indeed predicted The other main group, obtained 97% of the time in 20 740 explored trees, contained the DIDO PHD sequences clustered with the PHF and the CGBP ... in DNA regulation, in the context of chromatin stability, and to form part of higher complexes linked to active chromatin The DIDO1 gene has been linked to the split ends domain (SPEN) family of...
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THE MECHANISMS OF DNA REPLICATION pot
Ngày tải lên :
30/03/2014, 00:20
... lagging strand DNA synthesis [158] These factors influence the processivity and integrity of DNA synthesis Unwinding the ORI DNA to provide ssDNA as template for the DNA polymerases and to construct ... engagement of Cdc45 and GINS, both of the latter factors remain associated with the Mcm2-7 and are required for elongation of the nascent DNA strands following the initiation of DNA synthesis [136, ... 105, 108] Following the removal of the pri‐ mers, gaps are filled in, apparently by the action of the DNA polymerase δ and its cofactors, and the final DNA strands are ligated by DNA ligase I into...
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Báo cáo Y học: Nucleotide excision repair and chromatin remodeling pptx
Ngày tải lên :
31/03/2014, 21:21
... recognition of the damaged DNA; (b) excision of an oligonucleotide of 24–32 residues containing the damage from DNA by dual incision of the damaged strand on each side of the lesion; (c) filling in of the ... XPC-hHR23B, RPA, TFIIH, XPG and XPF–ERCC1) and other factors for repair DNA synthesis and ligation (PCNA, RFC, DNA polymerase a or d and DNA ligase I) [16–18] The molecular mechanisms of NER have been thoroughly ... because of DNA helix distortion in chromatin (3) The chromatin remodeling complexes are directed to the damage site by their interactions with XPC–hHR23B or other NER factors and then change chromatin...
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Báo cáo y học: "Transcriptional regulation of collagenase (MMP-1, MMP-13) genes in arthritis: integration of complex signaling pathways for the recruitment of gene-specific transcription factor" ppt
Ngày tải lên :
09/08/2014, 03:24
... dimerization of the 50 kDa NF-κB subunit (p50) and regulates the transcriptional activity of p50 to the transcription of an array of inflammatory genes, their direct regulation of MMP transcription ... given promoter vary among the MMP family members, and these variations influence the regulation of these genes [37] The interaction of AP-1 with other transcription factors may also regulate ... γ, and the retinoid x receptors α, β and γ) reduce MMP transcription by binding to Fos and Jun proteins at the AP-1 site, sequestering these proteins away from the promoter and/ or reducing the...
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Báo cáo y học: "Host proteins interacting with the Moloney murine leukemia virus integrase: Multiple transcriptional regulators and chromatin binding factors" pot
Ngày tải lên :
13/08/2014, 05:20
... fusions of MLV and HIV integrases and the clones interacted to varying degrees with MLV IN and HIV IN in these assays The isolation of chromatin remodeling factors (Enx-1, AF9, Brd2, Baz2b), DNA repair ... expression and propagation is the stable integration of the double-stranded DNA viral genome into the genome of their hosts The viral integrase protein is key in this essential step of the retroviral ... the basis of their phenotypes in the colony lift assay (intensity of activation based on blue color), the number of times the gene was isolated, and our interest in their proposed functions There...
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Báo cáo y học: "A novel scheme to assess factors involved in the reproducibility of DNA-microarray data" pdf
Ngày tải lên :
14/08/2014, 14:21
... preference of the reverse transcriptase enzyme for the Cy3 label and (ii) prolonged exposure to air and light of the dyes increasing the chance of oxidation and / or bleaching The main contributing factors ... data quality and sources of errors of DNA- microarrays Even in the case that 90 % of the data were preserved and the experiments were performed at challenging conditions, the coefficient of variance ... manufacturing DNA- microarrays to performing the actual experiments, systematic errors and / or bias in the data are introduced in each of the different steps The effects of various factors (e.g dye and...
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THE ROLE OF DNA METHYLATION IN REGULATING LHX3 GENE EXPRESSION
Ngày tải lên :
24/08/2014, 09:47
... members of the Department of Biochemistry and Molecular Biology, as well as members of the Department of Cellular and Integrative Physiology iii Abstract Raleigh Elizabeth Malik THE ROLE OF DNA METHYLATION ... proteins to form chromatin In the canonical model, nucleosomes, the basic unit of chromatin are composed of ~147 bp of DNA wrapped around a histone octamer composed of two copies each of the histones ... patterns of DNA methylation of the Lhx3 promoters, examines the effect of 5-aza-dc and TSA on Lhx3 gene expression, and identifies an association between the Lhx3 promoters and MeCP2 in the mouse...
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On the conformation of DNA confined in a nanochannel or absorbed at an interface
Ngày tải lên :
14/09/2015, 14:01
... understanding the physical and chemical phenomena that are important in the condensation of DNA In this thesis, we seek to uncover the behavior of single stranded and double stranded DNA molecules, ... condensation, irrespective of the nature of the condensing ligands and the specific surface interaction Another important part of this thesis is the study of single T4 -DNA molecules confined in ... which the biomolecule is subjected to a tensional force The role of the strength of the stretching force is the same as the value of the cross-sectional diameter of the nano-channel, in the sense...
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chromatin and chromatin remodeling enzymes, part b
Ngày tải lên :
11/04/2014, 01:17
... as the source of the chromatin Transfer the multigel to membrane and measure the in each running gel through use of Southern blotting Calculate the , 00 , and Re of the chromatin- band(s) of ... is, the y-axis.5,6,13 Using Eq (1), the Pe of each running gel is calculated from the and 00 and known Re (30.1 nm) of the T3 internal standard For the unknown band(s) of interest, the and ... in their appearance, then averages the grouped images, with the goal of having one group for each unique view of the particle The user then examines the group averages and the members of the...
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chromatin and chromatin remodeling enzymes, part c
Ngày tải lên :
11/04/2014, 01:19
... assays of histones and chromatin regulators, methods for the preparation and analysis of histone modifying and ATP-dependent chromatin remodeling enzymes, and assays for transcription and DNA repair ... one-quarter of the segregants will have knockouts of both chromosomal copies of the histone gene pairs and will be Uraþ due to the requirement for the plasmid These segregants then can be used to the ... repair of double-strand (ds) DNA breaks Double mutant combinations of hta1-S129A with mutations in genes involved in other ds break DNA repair pathway genes, RAD52 and yKU80, indicated that the...
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