α cleavage of aliphatic amines
Tài liệu Báo cáo khoa học: Post-translational cleavage of recombinantly expressed nitrilase from Rhodococcus rhodochrous J1 yields a stable, active helical form ppt
... restricts the number of degrees of freedom The twofold axis of the dimer model must coincide with the appropriate twofold axis of the stain envelope Thus, the two possible degrees of freedom (apart ... of 13 nm, a pitch of 7.7 nm and were of variable length 3D reconstruction of the fibres using the iterative, real-space method of Egelman [19] showed that the helices had 4.9 dimers per turn of ... [15,16] Activation of the enzyme on oligomerization of the dimers is typical of Rhodococcal nitrilases [17,18], and links formation of the quaternary structure to the activity Knowledge of interactions...
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Tài liệu Báo cáo khoa học: Inhibition of pneumococcal choline-binding proteins and cell growth by esters of bicyclic amines pptx
... CBP that catalyzes the cleavage of the N-acetylmuramoyl-l-alanine bond of the peptidoglycan backbone [8] It is involved in the separation of the daughter cells at the end of cell division and in ... inhibitor of the activity of LytA [23,24] and other CBPs [25] Moreover, addition of an excess of choline to culture media inhibits daughter cell separation and induces the formation of long chains ... concentration of choline capable of eluting C-LytA from a DEAE-cellulose column, mg of C-LytA samples were adsorbed onto mL of resin and subjected to washes with different concentrations of choline...
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Tài liệu Báo cáo khoa học: The role of the SEA (sea urchin sperm protein, enterokinase and agrin) module in cleavage of membrane-tethered mucins pdf
... cotranslational cleavage event in vivo or that the structures of the hybrid molecules not enable efficient cleavage Results Mutation of the FRPG ⁄ SVVV motif does not completely inhibit cleavage of MUC1FDTR ... the role of the SEA domain in cleavage of membrane-tethered mucins we carried out site-directed mutagenesis of the FRPG ⁄ SVVV site and evaluated hybrid mucins in which the SEA module of MUC1 ... SVVV motif does not completely prevent cleavage of MUC1 Though mutation of the Phe, Gly and Ser residues has a significant effect on the efficiency of the cleavage reaction, it is not completely...
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Tài liệu Báo cáo khoa học: Cleavage of nonphenolic b-1 diarylpropane lignin model dimers by manganese peroxidase from Phanerochaete chrysosporium Evidence for a hydrogen abstraction mechanism docx
... over 90% of the added diarylpropane I was oxidized in the presence of Tween 80, while in the absence of Tween 80, 24% of the diarylpropane I was oxidized In addition, while about 30% of the diarylpropane ... Amount of products formed (mol percentage) are shown conducted in the absence of Tween 80 Finally, in the presence of Tween 80, oxidation of a-keto diarylpropane (X) resulted in the formation of ... investigating the nature of the oxidant in the reaction of the diarylpropane I by MnP in the absence of Fig Proposed hydrogen abstraction mechanism for the oxidative cleavage of the a-keto diarylpropane...
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Báo cáo khoa học: Specific cleavage of the DNase-I binding loop dramatically decreases the thermal stability of actin pot
... error of the given values of Tm did not exceed ±0.2 °C The relative error of the given values of DHcal did not exceed ±10% G-actin Results Effects of the D-loop cleavage on the thermal unfolding of ... The measurement of actin concentration in solution: a comparison of methods Anal Biochem 62, 66–74 Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage ... role in stabilization of the actin filament [17,30,47] The cleavage of the D-loop between Gly42 and Val43 impairs these contacts [47], and this may explain why the cleavage of the D-loop in its...
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Báo cáo khoa học: The Mycobacterium tuberculosis ORF Rv0654 encodes a carotenoid oxygenase mediating central and excentric cleavage of conventional and aromatic carotenoids doc
... mediates the symmetrical cleavage of b-carotene at the C15-C15¢ site, as well as the asymmetrical cleavage of the C13-C14 or the C13¢-C14¢ double bond To test the cleavage of hydroxylated C40-carotenoids, ... analyses of the lutein cleavage products The cleavage products of the incubation with lutein were purified by HPLC and subjected to LC-MS analyses The products showed the molecular ions [M+H]+ of m ... the cleavage of the C9-C10¢ double bond of bcarotene leading to b-apo-10¢-carotenal and b-ionone The novelty of MtCCO is mirrored by its capability to act as a central, as well as an excentric cleavage...
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Báo cáo khoa học: Mutagenesis at the a–b interface impairs the cleavage of the dystroglycan precursor doc
... basis of these results, we hypothesized that perturbation of the network of interactions that is likely to stabilize the a–b interface within the DG complex may interfere with the cleavage of the ... N-terminal portion of this linear sequence, namely W551, F554 and N555, in order to evaluate the contribution of each amino acid sidechain to the stability of the a–b interface [26] As none of these three ... of mutants of murine DG fused to GFP The a- and b-subunits of mammalian DG contain several well-conserved domains: (a) the N-terminal domain, the mucin-like region and the C-terminal region of...
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Báo cáo khoa học: In vitro gamma-secretase cleavage of the Alzheimer’s amyloid precursor protein correlates to a subset of presenilin complexes and is inhibited by zinc potx
... universal characteristic of AD pathology is the post-mortem detection of Ab plaques, thus confirming the pathological relevance of c-secretase cleavage of APP Since only a small subset of AD cases are ... Regardless of the counterion, zinc inhibited cleavage of MC99-3FLAG with comparable IC50 values of 22 and lm Zn Using two assay systems, these results show that zinc can inhibit in vitro c-secretase cleavage ... greater detection of c-secretase activity Lastly, zinc causes structural changes in a c-secretase substrate and acts as an inhibitor of csecretase cleavage of APP Results Design of a novel APP c-secretase...
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Báo cáo khoa học: Detection and characterization of a novel extracellular fungal enzyme that catalyzes the specific and hydrolytic cleavage of lignin guaiacylglycerol b-aryl ether linkages pdf
... Enzymic reaction and analysis of metabolites Reactions were initiated by the addition of 100 ll of a solution of enzyme (200 lgỈmL)1) to 0.9 mL of a solution of 50 lg of substrate in 10% (v/v) dimethyl ... by using a GOU fluorometric assay of b-aryl ether cleavage One unit of enzyme activity was defined as the amount of b-aryl ether cleavage enzyme that released ng of 4-methylumbelliferone (4MU) per ... of 0.1 g of HP or M to 1.0 mL of VM containing 10 lg of GOU After incubation for 12 h at 28 °C, the reaction mixture was centrifuged at 15 000 g for 10 Reactions were terminated by addition of...
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Reductive Cleavage of N-O Bonds in Hydroxylamines and Hydroxamic Acid Derivatives Using Samarium Diiodide pot
... Thus, the cleavage of O-alkylhydroxylamines at low temperature is not feasible Although the direct isolation of low molecular weight amines was not practical the isolation of primary amines derived ... reductive cleavage of N-O bonds of simple hydroxylamines are shown in Table Some of the yields reported are for the acetamide or trifluoroacetamide instead of the primary amines, due to problems ... Utilization of N-acetylated O-alkylhydroxylamines may offer some advantages over simple O-alkylhydroxylamines in cases where chemoselective reduction of an N-O bond is required in the presence of another...
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Báo cáo khoa học: Cleavage of focal adhesion proteins and PKCd during lovastatin-induced apoptosis in spontaneously immortalized rat brain neuroblasts ppt
... the cleavage of both FAK (106 ± 12% of intact FAK) and p130Cas (91 ± 11% of intact p130Cas) proteins Mevalonate alone had no effect on the integrity of focal adhesion proteins (101 ± 11% of intact ... establish the role of the mevalonate pathway in the physiological growth and development of cells from the central nervous system Results Effect of lovastatin on the cleavage of focal adhesion ... 36 ± 11% of intact p130Cas, respectively, remaining at this concentration (expressed as percentage of noncleavaged proteins in untreated cells) Proteolysis of FAK generated two main cleavage...
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Báo cáo khoa học: In vitro and in vivo self-cleavage of Streptococcus pneumoniae signal peptidase I pot
... 5.0 software from Molecular Dynamics Specific activities of selfcleavage in the presence or absence of phospholipid were calculated according to the cleavage of SPase I at concentration of mgỈmL)1 ... stimulates the self -cleavage of SPase I Self -cleavage sites of S pneumoniae SPase I resemble signal peptide cleavage sites Although signal peptides of secreted proteins not show a great deal of sequence ... product of the self -cleavage lost its ability to cleave its substrates Therefore, the self -cleavage of SPase I is believed to inactivate the activity of the enzyme In vivo self -cleavage of S pneumoniae...
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Báo cáo Y học: Thermodynamics and kinetics of the cleavage of DNA catalyzed by bleomycin A5 A microcalorimetric study pdf
... the cleavage Ó FEBS 2002 Thermokinetics of DNA cleavage catalyzed by BLM-A5 (Eur J Biochem 269) 2855 Table Thermodynamic and kinetic data of the cleavage of calf thymus DNA by a mixture of BLM-A5, ... Thermodynamics of the binding of ADM and (1,10-phenanthroline)-copper to DNA Fig A comparison of the UV and visible spectrum of BLM-A5 after the cleavage of calf thymus DNA by a mixture of BLM-A5, ... UV-vis spectra of BLM-A5 Figure 3A compares the UV and visible spectrum from 250 to 500 nm of BLM-A5 after the cleavage of calf thymus DNA by a mixture of BLM-A5, Fe2+ and O2 with those of the five...
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Báo cáo hóa học: "Dermal absorption of aromatic amines in workers with different skin lesions: a report on 4 cases" doc
... influence of smoking on internal exposure by measurement of cotinine in the urine of the workers In view of the explanatory nature of our data, we used the RIE index to compare the values of external ... internal exposure of about the last months in accordance with the lifetime of the erythrocytes – is a powerful tool for the surveillance of occupationally exposed workers Only the approach of biological ... P: Determination of the levels of aromatic amines in indoor and outdoor air in Italy Chemosphere 2001, 43:355-361 Luceri F, Pieraccini G, Moneti G, Dolara P: Primary aromatic amines from side-stream...
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Báo cáo toán học: " Metabolite proving fungal cleavage of the aromatic core part of a fluoroquinolone antibiotic" pdf
... fungal cleavage of the aromatic fluoroquinolone core for the first time Hence, two decades after the emergence of the notion of the nonbiodegradability of fluoroquinolones, fungal degradation of ... to F-5 of enrofloxacin (Wetzstein et al 1997) or ciprofloxacin (Wetzstein et al 1999), for which hydroxylation of position C-5 was indistinguishable from hydroxylation of C-8 Degradation of the ... chemical degradation of enrofloxacin, ciprofloxacin or other FQs, cleavage of the aromatic core could not yet be proved either, if based on metabolite identification Work on ciprofloxacin confirmed...
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báo cáo hóa học:" Metabolite proving fungal cleavage of the aromatic core part of a fluoroquinolone antibiotic" docx
... fungal cleavage of the aromatic fluoroquinolone core for the first time Hence, two decades after the emergence of the notion of the nonbiodegradability of fluoroquinolones, fungal degradation of ... to F-5 of enrofloxacin (Wetzstein et al 1997) or ciprofloxacin (Wetzstein et al 1999), for which hydroxylation of position C-5 was indistinguishable from hydroxylation of C-8 Degradation of the ... chemical degradation of enrofloxacin, ciprofloxacin or other FQs, cleavage of the aromatic core could not yet be proved either, if based on metabolite identification Work on ciprofloxacin confirmed...
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Báo cáo y học: " Caspase-mediated cleavage of the exosome subunit PM/Scl-75 during apoptosis." pot
... effect of the activation of other proteases during apoptosis The cleavage of PM/Scl-75 occurs in the Cterminal part of the protein at the unconventional caspase cleavage site IILD369↓G However, cleavage ... control for the inhibitory activity of the tetrapeptide inhibitors, the cell extracts were also analyzed for cleavage of U1-70K and Sm-F The inhibition of cleavage of both caspase substrates seemed ... antiPM/Scl-75 antiserum was used to analyze the cleavage of PM/ Scl-75 in these cells The cleavage of PM/Scl-75 was completely inhibited in the presence of or 20 μM Ac-YVAD-CMK, Z-DEVD-FMK or Z-IETD-FMK...
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Human parvovirus B19 nonstructural protein NS1 enhanced the expression of cleavage of 70 kDa U1-snRNP autoantigen ppt
... 1.5 μM of MgCl2, 200 μM of dNTPs, μM of each primer and 2.5 units of Taq DNA polymerase (Promega, Madison, Wisconsin, USA) using a Perkin-Elmer Gene Amp PCR system 2400 Each cycle consists of denaturation ... B19-NS1induced cleavage of 70 kDa U1-snRNP, however, it could suggest the association between B19-NS1 induced cleavage of 70 kDa U1-snRNP by B19-NS1 and autoimmunity Although the precise role of NS1 ... protein utilizing the principle of protein-dye binding Anal Biochem 1976, 72:248-254 32 Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 1970,...
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Báo cáo y học: " High cell density and latent membrane protein 1 expression induce cleavage of the mixed lineage leukemia gene at 11q23 in nasopharyngeal carcinoma cell line" ppt
... idea of the project, the design of the study, interpretation of data and writing of manuscript PHCY have been involved in the detailed experimental design, acquisition of data, interpretation of ... base of the chromatin loop, results in the formation of HMW DNA of 50 - 300 kb [32] In this study, the MLL cleavage sites observed in the NPC cells localized within the MAR/SAR sequence of the ... Prof Leroy Liu, USA), harboring a section of the MLL bcr was used as DNA template Briefly, PCR reaction was carried out with 10 pg of DNA template, 50 pmol each of the primers, 200 μM each of...
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Báo cáo y học: "Cleavage of functional IL-2 receptor alpha chain (CD25) from murine corneal and conjunctival epithelia by MMP-9" pptx
... worth noting that the role of MMP-9 in the cleavage of soluble CD25 is still controversial High doses of MMP9 (1 ug/mL) were shown to downregulate the expression of IL-2Rα on activated human T cells ... cultures [8] IL-2 treatment of these intestinal epithelial cells was noted to stimulate production of TGFβ [9] IL-2Rα is an essential component of the IL-2R IL-2Rα knock-out mice are phenotypically ... washings from both eyes of two mice pooled (4 μL) in mouse CD25 assay buffer (6 μL) There were a total of samples from each group of mice CD25 concentrations in tear washings of NS, DS5 and DS5+Doxy...
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