x5 exhibits remarkable degree of breadth and potency against hiv 1

Báo cáo y học: "Comparative biochemical analysis of recombinant reverse transcriptase enzymes of HIV-1 subtype B and subtype " ppt

Ngày tải lên : 13/08/2014, 01:20

... RT C RT -18 -15 -7 0.5 0 1 15 1. 5 0 1 15 1 0.5 1. 5 1. 5 0.5 15 1 15 (min) 0.5 1. 5 Figure RNase H activity of HIV- 1 subtype B and C recombinant wild type RTs (A) Graphic representation of the substrate ... Subtype B HIV- 1 Subtype C HIV- 1 ZDV 12 .9 ± 5.9 34.8 ± 12 .9 3TC 45.4 ± 16 .4 10 5.9 ± 40.2 TFV 334.8 ± 14 0.9 304.8 ± 93 .1 NVP 50.2 ± 29.6 17 0.4 ± 79.5 EFV 0.6 ± 0.2 1. 3 ± 0.4 0.9 ± 0.4 0.5 ± 0. 01 a To ... heterogeneous HIV- 1 RNA template/primer, 10 units of RT, and variable amounts of RT inhibitors was included in 50-μl reaction volumes In each reaction, 0, 0 .1, 0.3, 1. 0, 3.0, 10 .0, 30.0 and 10 0.0 μM of...

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Báo cáo Y học: The processivity and ﬁdelity of DNA synthesis exhibited by the reverse transcriptase of bovine leukemia virus pot

Ngày tải lên : 31/03/2014, 21:21

... 1/ 3460 1/ 9000, 1/ 32 250 1/ 41 500 and 1/ 52 200 1/ 75 000; and for MLV RT: 1/ 25 000, < 1/ 300 000 and < 1/ 300 000, all for the formation of AÆC, AÆG, and AÆA mispairs, respectively [16 ,20] Extension of ... Km (lM) Vmax (%Æmin )1) Fext AÆT AÆC AÆG AÆA 0.037 86 42 68 29.9 13 .6 10 .2 11 .4 1/ 5 ,10 0 1/ 3,400 1/ 4,800 higher than that of HIV- 1 RT (46%) However, by calculating the level of extension after correcting ... trap 12 .3 15 .4 27.3 5.6 60.6 30.2 1. 4 1. 0 0 .1 32.7 14 .9 13 .3 20.3 27.6 76 .1 8.9 7.5 6.2 12 .8 35.4 10 .2 8 .1 18.9 35.4 72.6 46 25.8 0.3 0.3 72.4 53.9 46.3 99.7 Table Quantitative analysis of DNA...

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the nitro group in organic synthesis 2001 by noboru ono

Ngày tải lên : 09/05/2014, 17:07

... O O O3, SiO2, 78 °C CrS2, TBHP Nitro compound Yield (%) Ref 69 95 10 8 11 1 85 11 0 75 11 5 82 92 11 9 11 0 12 97 66 10 8 11 1 10 8 10 9 NO2 NO2 CH2NO2 CH=NOH n-C4H9NH2 t-C4H9NH2 O3, SiO2, 78 °C O O, ... Zr(Ot-Bu)4 76 n-C4H9NO2 65 84 63 10 8 11 1 11 5 t-C4H9NO2 83 90 70 64 11 7 11 1 10 8 11 9 2.2 23 SYNTHESIS OF NITRO COMPOUNDS BY OXIDATION NO2 NOH BzOMoO(O2)2– (2. 61) CH3CN, 40 ºC 55% NO2 BzOMoO(O2)2– ... of Nitro Compounds 2 .1 Nitration of Hydrocarbons / 2 .1. 1 Aromatic Compounds / 2 .1. 2 Alkanes / 2 .1. 3 Activated C-H Compounds / 10 2 .1. 4 Alkenes / 11 2 .1. 5 Synthesis of =-Nitro Ketones / 16 2 .1. 6...

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Báo cáo y học: "Protein synthesis molecule by molecule" pot

Ngày tải lên : 14/08/2014, 16:21

... networks Nature 2004, 427: 415 - 418 Genome Biology 2006, 7:2 21 http://genomebiology.com/2006/7/6/2 21 10 11 13 15 16 17 18 deposited research 14 reports 12 Golding and Cox 2 21. 3 reviews refereed research ... Natl Acad Sci USA 2004, 10 1 :15 9 21- 15926 Yu J, Xiao J, Ren X, Lao K, Xie XS: Probing gene expression in live cells, one protein molecule at a time Science 2006, 311 :16 00 -16 03 Nagai T, Ibata K, ... to a random burst of proteins, with a geometrical distribution of burst sizes, but rather, they are a direct result of the randomness of mRNA production, with each random burst of RNA production...

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Tài liệu Báo cáo khoa học: Kinetics of dextran-independent a-(1 ﬁ 3)-glucan synthesis by Streptococcus sobrinus glucosyltransferase I pdf

Ngày tải lên : 14/02/2014, 22:20

... 70 ± 27 ± 26 ± 16 ± ND 5± Acc kcat ⁄ Km (s )1 mM )1) 31 kcat (s )1) 0.56 2.0 2.2 4.6 41 41 69 65 59 40 ± ± ± ± 0.27 0.4 0.3 1. 2 11 ± ± ± ± ± ± ± Acc Km (mM) 25 12 19 78 12 33 17 13 ± ± ± ± ± ± Acc ... OMZ176 J Gen Microbiol 12 9, 7 51 754 11 Kato C, Nakano Y, Lis M & Kuramitsu HK (19 90) Carboxyl-terminal deletion analysis of the Streptococcus 15 16 17 18 19 20 21 22 23 24 mutans glucosyltransferase-I ... Biochemical and molecular characterization of Lactobacillus reuteri 12 1 reuteransucrase Microbiology 15 0, 2099– 211 2 Chambert R, Treboul G & Dedonder R (19 74) Kinetic studies of levansucrase of Bacillus...

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Tài liệu Báo cáo khoa học: Enzymatic and electron paramagnetic resonance studies of anabolic pyruvate synthesis by pyruvate: ferredoxin oxidoreductase from Hydrogenobacter thermophilus doc

Ngày tải lên : 16/02/2014, 09:20

... strains and growth conditions Escherichia coli JM109 and BL 21( DE3) were used as hosts for derivatives of pUC19 and pET21c, respectively E coli MV 118 4 was used as a host for the expression of T caldophilus ... Puriﬁcation of ﬁve components from Clostridium thermoaceticum which catalyze synthesis of acetate from pyruvate and methyltetrahydrofolate Properties of phosphotransacetylase J Biol Chem 256, 11 137 11 144 ... activity of POR was determined to be 0.23 UÆmg )1 with 10 lm Fd1, or 0 .19 UÆmg )1 with 10 lm Fd2 These values were comparable to those of the oxidative decarboxylation of pyruvate with Fd1 or Fd2...

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Tài liệu Báo cáo khoa học: Interferon-a induces sensitization of cells to inhibition of protein synthesis by tumour necrosis factor-related apoptosis-inducing ligand ppt

Ngày tải lên : 19/02/2014, 06:20

... (p53/55) p18 Band intensity (arbitrary units) p 41/ 43 10 0 p 41/ p43 p18 HeLa cells (14 3%) 75 50 (88%) (90%) (10 3%) 25 p53/p55 p 41/ p43 p18 Fig Effects of IFNa and TRAIL on levels and activation of caspase-8 ... 720 18 0 360 540 720 +TRAIL (4h) Sub-G1 1. 6% 0 200 400 600 200 DNA content 600 Ce ll c ount Sub-G1 6 .1% + IFNα +TRAIL (16 h) 18 0 360 540 720 +TRAIL (16 h) 18 0 360 540 720 400 DNA content Sub-G1 16 .0% ... state of phosphorylation of eIF2a and 4E-BP1 (A) MCF-7 cells were grown for 72 h in the absence or presence of human IFNa2b (10 00 UÆmL )1) and further treated with or without TRAIL (16 7 ngÆmL )1) ...

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Tài liệu Báo cáo khoa học: Stimulation of poly(A) synthesis by Escherichia coli poly(A)polymerase I is correlated with Hfq binding to poly(A) tails ppt

Ngày tải lên : 19/02/2014, 16:20

... Genes Dev 14 , 11 09 11 18 11 Moll I, Afonyushkin T, Vytvytska O, Kaberdin VR & Blasi U (2003) Coincident Hfq binding and RNase E cleavage sites on mRNA and small regulatory RNAs RNA 9, 13 08 13 14 462 ... without (lanes & 8) and with increasing Hfq concentrations; nM (lanes & 9), 10 nM (lanes & 10 ), 20 nM (lanes & 11 ), 40 nM (lanes & 12 ), 80 nM (lanes & 13 ) and 16 0 nM (lanes & 14 ) Incubation was ... Natl Acad Sci USA 97, 11 966 11 9 71 45 de Haseth PL & Uhlenbeck OC (19 80) Interaction of Escherichia coli host factor protein with oligoriboadenylates Biochemistry 19 , 613 8– 614 6 46 Brescia CC, Mikulecky...

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Tài liệu Báo cáo Y học: Dinucleoside polyphosphates stimulate the primer independent synthesis of poly(A) catalyzed by yeast poly(A) polymerase ppt

Ngày tải lên : 21/02/2014, 01:21

... (PM98/ 012 9; BMC2002-00866) and Comunidad de Madrid (08/00 21. 1/20 01) H.O was supported by a Fellowship from Fundacao para a Ciencia e a Tecnologia (SFRH/BD/ ¸ ˜ ˆ 14 77/2000) 10 11 12 13 14 15 16 17 18 ... Eur J Biochem 37, 31 40 Edmonds, M (19 90) Polyadenylate polymerases Meth Enzymol 18 1, 16 1 17 0 Edmonds, M & Abrams, R (19 60) Polynucleotide biosynthesis: formation of a sequence of adenylate units ... 26 715 –26720 Chen, J & Moore, C (19 92) Separation of factors required for cleavage and polyadenylation of yeast pre-mRNA Mol Cell Biol 12 , 3470–34 81 Lingner, J., Kellermann, J & Keller, W (19 91) ...

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Tài liệu Báo cáo Y học: Phosphatidylinositol synthesis and exchange of the inositol head are catalysed by the single phosphatidylinositol synthase 1 from Arabidopsis docx

Ngày tải lên : 22/02/2014, 04:20

... the two sets of PtdIns molecules: the C16:0/C17c + C18 :1/ C17c and C16:0/C19c peaks are characteristic of E coli (A.-M Justin, unpublished data) whereas the C16:0/C18:2 and C18:0/C18:2 PtdIns are ... lM CMP –PIS 2 019 ± 71 )10 8 ± 10 3 ± 13 )1 125 ± 11 09 ± 13 8 0 exogenous PtdIns De novo synthesis was measured in the incubation conditions described above, in the presence of CDP-DAG and myo-inositol ... constant K1 of · 10 11 M )1 for manganese at pH 8.0 [18 ] At 7.5 mM EDTA, when the calculated concentration of free manganese ions is in the submicromolar range, a synthesis activity of 1. 53 nmol...

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Báo cáo khoa học: Heterologous synthesis of cytochrome c¢ by Escherichia coli is not dependent on the System I cytochrome c biogenesis machinery ppt

Ngày tải lên : 06/03/2014, 00:20

... Journal 278 (2 011 ) 23 41 2348 ª 2 011 The Authors Journal compilation ª 2 011 FEBS 2347 Biogenesis of cytochrome c¢ 10 11 12 13 14 15 16 17 18 19 H Inoue et al requires coexpression of cmm genes in ... c550 probe pathway of c-type cytochrome biogenesis in Escherichia coli Mol Microbiol 19 , 11 93 12 04 FEBS Journal 278 (2 011 ) 23 41 2348 ª 2 011 The Authors Journal compilation ª 2 011 FEBS ... dichroism and optical rotatory dispersion Biochemistry 11 , 412 0– 413 1 Ren Z, Meyer T & McRee DE (19 93) Atomic structure of a cytochrome c¢ with an unusual ligand-controlled ˚ dimer dissociation at 1. 8...

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Báo cáo khoa học: Iron regulatory protein-independent regulation of ferritin synthesis by nitrogen monoxide pot

Ngày tải lên : 07/03/2014, 12:20

... effects of NO+ on Ft synthesis A 10 0μM SNP A 15 30 1. 0 35S M Mikhael et al 60 1. 4 3.2 12 0 18 0 (min) H+L 7 .1 15.7 24.8 (D.A.) 10 0μM SNP B B 15 30 60 12 0 18 0 (min) IRP IRP IRP +β-ME IRP Fig Effects of ... atmosphere of 95% air and 5% CO2 at 37 °C in DMEM containing 10 % fetal bovine serum, extra l-glutamine (300 lgÆmL )1) , sodium pyruvate (11 0 lgÆmL )1) , penicillin (10 0 unitsÆmL )1) , and streptomycin (10 0 ... a 10 mL linear sucrose gradient (15 –40% sucrose w ⁄ v supplemented with 10 mm Tris ⁄ HCl, pH 7.5, 14 0 mm NaCl, 1. 5 mm MgCl2, 10 mm dithiothreitol, 10 0 lgÆmL )1 cycloheximide, and 0.5 mgÆmL )1 heparin)...

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Báo cáo khoa học: 15 N-Labelled proteins by cell-free protein synthesis Strategies for high-throughput NMR studies of proteins and protein–ligand complexes doc

Ngày tải lên : 07/03/2014, 12:20

... (2006) 415 4– 415 9 ª 2006 The Authors Journal compilation ª 2006 FEBS 15 K Ozawa et al 10 11 12 13 14 15 16 17 Nureki O & Yokoyama S (20 01) Selenomethionine incorporation into a protein by cell-free ... demonstrated for each of the 19 nonproline residues [18 ] Time and expense can be drastically reduced by use of cell-free systems [11 ,18 , 21] , opening many avenues for strategic applications of selectively ... the [15 N]-HSQC spectrum [11 ] Similarly, the presence of ﬂexible polypeptide segments in the protein construct can be assessed by the observation of intense and narrow [15 N]-HSQC crosspeaks Often,...

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Báo cáo khoa học: Induction of PPARb and prostacyclin (PGI2) synthesis by Raf signaling: failure of PGI2 to activate PPARb potx

Ngày tải lên : 07/03/2014, 12:20

... J Biol Chem 272, 14 5 01 14 504 29 Blaine SA, Wick M, Dessev C & Nemenoff RA (20 01) Induction of cPLA2 in lung epithelial cells and nonsmall cell lung cancer is mediated by Sp1 and c-Jun J Biol ... generation Prostaglandins Other Lipid Mediat 71, 1 22 32 Forman BM, Tontonoz P, Chen J, Brun RP, Spiegelman BM & Evans RM (19 95) 15 -Deoxy-delta 12 , 14 -prostaglandin J2 is a ligand for the adipocyte ... Company), UO126 (Promega, Mannheim, Germany) Cell culture NIH3T3, N-BxB-ER, HEK293 and CHO cells were cultured in DMEM supplemented with 10 % fetal bovine serum, 10 0 UÆmL )1 penicillin and 10 0 lgÆmL )1 streptomycin...

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Báo cáo khoa học: Characterization of the tRNA and ribosome-dependent pppGpp-synthesis by recombinant stringent factor from Escherichia coli pot

Ngày tải lên : 07/03/2014, 16:20

... (10 lm), thiostrepton (10 lm) and viomycin (0 .1 10 mm) before addition of nucleotides and SF The reactions were stopped by the addition of lL 88% (v ⁄ v) formic acid, incubated on ice for 15 and ... 3¢-polyphosphates by the 694 15 16 17 18 ribosomal wash of stringent Escherichia coli Proc Natl Acad Sci USA 70, 214 5– 214 8 Haseltine WA & Block R (19 73) Synthesis of guanosine tetra- and penta phosphate ... samples and Endogenous activity of SF (lanes 6, 14 ) in the presence of 10 mM viomycin (lane 7) and 0.5 mM tetracycline (lane 15 ) Ribosomes were incubated with antibiotics before the addition of SF and...

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Báo cáo khoa học: Oligosaccharide synthesis in Fibrobacter succinogenes S85 and its modulation by the substrate potx

Ngày tải lên : 07/03/2014, 17:20

... of their utilization were 0 .10 lmolÆmg of protein )1 min )1 and 0.06 lmolÆmg of protein )1 min )1, respectively, while the rates were 0.05 lmolÆmg of protein )1 min )1 and 0.03 lmolÆmg of protein )1 min )1 ... mixture of 470 lL of 99.98% D2O, 20 lL of 10 mm TSP-d4 and 10 lL of 50 mm 1- O-methyl-b-d-xylopyranose Basic quantiﬁcation of H1 signals at d 5.46 and 5.43–5. 41 (each representing a mixture of metabolites, ... glucose -1- phosphate; MD1P, maltodextrin -1- phosphate; MD1Pa, Glc1P unit of MD1P; MD1Pb, Glc unit neighbouring the Glc1P of MD1P; MD, linear maltodextrins; MDt, the terminal Glc unit of MD; MDint, internal...

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Báo cáo Y học: Inhibition of hyaluronan synthesis in Streptococcus equi FM100 by 4-methylumbelliferone doc

Ngày tải lên : 08/03/2014, 09:20

... 7.74 11 .27 20.02 26.37 12 .44 7.64 6.33 0.22 0.83 0.98 0.28 0.98 0.99 0 .18 0.50 0. 41 3 .12 3. 41 6.04 10 .03 18 . 91 29.30 14 .39 8.06 6.65 1. 48 0.52 0.67 0.84 1. 45 1. 09 0 .14 0.88 1. 00 added in the cell-free ... detecting absorbance at a wavelength of 215 nm The molecular sizes of standard HA were 1. 0, 3.0, 4 .1, 8.0, 12 and 19 · 10 5 [29] Preparation of a membrane-rich fraction and solubilization The membrane-rich ... (mM) 1. 0 2.0 Peak number Average % SEM Average % SEM Average % SEM A B C D E F G H I 4.52 6.52 9.59 14 . 61 20.59 18 .78 9.56 7. 91 7.92 0.72 0.43 0. 51 0.20 1. 50 1. 38 0.86 0.28 0.97 3.36 4.85 7.74 11 .27...

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Báo cáo Y học: Concerted regulation of free arachidonic acid and hormone-induced steroid synthesis by acyl-CoA thioesterases and acyl-CoA synthetases in adrenal cells pdf

Ngày tải lên : 08/03/2014, 09:20

... incubation of [1- 14C]AA-CoA in the presence of the puriﬁed enzyme resulted in the release of AA Km and Vmax values obtained for the reactions were 4 .1 and lM and 948 and 19 3 nmolÆmin )1 mg )1 for CTE-I and ... Biochem 269) 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 intramitochondrial cholesterol translocator J Biol Chem 262, 918 1– 918 8 Miller, W.L (19 88) Gene conversions, deletions and polymorphisms ... 7.5 and 4.5 lM in the presence of 0 .1, 0.5 and lM triacsin C, respectively The apparent IC50 for triacsin C is also reduced from 5.5 lM to 1. 75, 0.275 and 0 .1 lM in the presence of 5, 10 and...

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Báo cáo khoa học: DNA mediated disassembly of hRad51 and hRad52 proteins and recruitment of hRad51 to ssDNA by hRad52 pot

Ngày tải lên : 16/03/2014, 14:20

... effect of DNA on hydrodynamic radius (Rh) of hRad 51 hRad 51 (1 lM) was incubated with 0, 1, 3, and lM of oligo PUC+ (in the absence of any nucleotide cofactor) followed by the measurement of hydrodynamic ... absence (lane 1 5) or presence of mM ATP (lane 6 10 ) and analysed by native PAGE (6% acrylamide) followed by silver staining Lane and has hRad 51 (10 lM) without DNA and lane 11 had hRad52 (10 lM) with ... Effect of DNA on the interaction of hRad 51 hRad52 (A) hRad52 selectively interacts with higher oligomeric forms of hRad 51 in the absence of DNA Rad 51 (10 lM) was incubated with 0, 2.5, 5.0 and 10 ...

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Báo cáo khoa học: Methylene analogues of adenosine 5¢-tetraphosphate Their chemical synthesis and recognition by human and plant mononucleoside tetraphosphatases and dinucleoside tetraphosphatases pot

Ngày tải lên : 16/03/2014, 14:20

... angustifolius) 0.05 0 .18 0.0 016 0.0023 1. 3 0.25 40 10 0 .16 16 0.40 0.36 0.030 0.034 0.07 0.62 53 n.d n.d n.d n.d and pCH2pppA appear to be the strongest inhibitors of both the human and plant enzymes ... Med 11 , 223–227 Kuprianov VV, Ferretti JA & Balaban RS (19 86) Muscle adenylate kinase catalyzes adenosine 5¢-tetraphosphate synthesis from ATP and ADP Biochim Biophys Acta 869, 10 7 11 1 Randerath ... properties RNA 9, 11 08– 11 22 41 Maier L & Gredig R (19 69) Organophosphorus compounds XXXVI Preparation and properties of bis(dialkoxyphosphonylmethyl)-, bis(alkoxyphosphinylmethyl)-, and bis(oxophosphonarylmethyl)...

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