x5 does not inhibit transduction by vsv g pseudotyped hiv 1 vector
Báo cáo y học: "Comparative biochemical analysis of recombinant reverse transcriptase enzymes of HIV-1 subtype B and subtype " ppt
... 5’-GGATTAACTGCGAATCGTTCTAGCT-3’; dPR, 5’-GTCCCTGTTCGGGCGCCA-3’; ppt17D, 5’-TTAAAAGAAAAGGGGGG-3’; pp57D, 5’-CGTTGGGAGTGAATTAGCCCTTCCAGTCCCCCCTTTTCTTTTAAAAAGTGGCTAAGA-3’; kim40R, 5’-AAGCTTGGCTGCAGAATATTGCTAGCGGGAATTCGGCGCG-3’; ... http://www.retrovirology.com/content/7 /1/ 80 Page of 11 A -1 -10 -20 Kim40R 5’-AAGCUUGGCUGCAGAAUAUUGCUAGCGGGAAUUCGGCGCG-3’ Kim32D GACGTCTTATAACGATCGCCCTTAAGCCGCGC 3’-GACGTCTTATAACGATCGCCCTTAAGCCGCGC-5’ B RNase H activity ... activity w/ trap B RT C RT -18 -15 -7 0.5 0 1 15 1. 5 0 1 15 1 0.5 1. 5 1. 5 0.5 15 1 15 (min) 0.5 1. 5 Figure RNase H activity of HIV- 1 subtype B and C recombinant wild type RTs (A) Graphic representation...
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Báo cáo Y học: The processivity and fidelity of DNA synthesis exhibited by the reverse transcriptase of bovine leukemia virus pot
... (%Æmin )1) Fext AÆT AÆC A G AÆA 0.037 86 42 68 29.9 13 .6 10 .2 11 .4 1/ 5 ,10 0 1/ 3,400 1/ 4,800 higher than that of HIV- 1 RT (46%) However, by calculating the level of extension after correcting for ... the text Pair or mispairs formed Km (lM) Vmax (%Æmin )1) Fins AÆT AÆC A G AÆA 0.004 28 45 55 25 15 .1 4.5 1. 1 1/ 11 600 1/ 62 500 1/ 300 000 [16 ,19 – 21] The assays were performed with templateprimers ... explaining the extensive genetic heterogeneity of both HIV- 1 and HIV- 2, which affects viral pathogenesis, the rapid emergence of drug-resistant variants and, hence, the progression of AIDS [2 ,10 ,11 , 31] ...
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the nitro group in organic synthesis 2001 by noboru ono
... O O O3, SiO2, 78 °C CrS2, TBHP Nitro compound Yield (%) Ref 69 95 10 8 11 1 85 11 0 75 11 5 82 92 11 9 11 0 12 97 66 10 8 11 1 10 8 10 9 NO2 NO2 CH2NO2 CH=NOH n-C4H9NH2 t-C4H9NH2 O3, SiO2, 78 °C O O, ... Zr(Ot-Bu)4 76 n-C4H9NO2 65 84 63 10 8 11 1 11 5 t-C4H9NO2 83 90 70 64 11 7 11 1 10 8 11 9 2.2 23 SYNTHESIS OF NITRO COMPOUNDS BY OXIDATION NO2 NOH BzOMoO(O2)2– (2. 61) CH3CN, 40 ºC 55% NO2 BzOMoO(O2)2– ... der Organische Chemie, edited by E Muller, and Georg Thieme Verlag, Stuttgardt, vol 10 /1 (19 71) and vol E16D /1 (19 92) The Chemistry of the Nitro and Nitroso Group (parts and 2), edited by H Feuer,...
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Báo cáo y học: "Protein synthesis molecule by molecule" pot
... Biophys Acta 19 53, 11 :383-395 Paulsson J: Summing up the noise in gene networks Nature 2004, 427: 415 - 418 Genome Biology 2006, 7:2 21 http://genomebiology.com/2006/7/6/2 21 10 11 13 15 16 17 18 deposited ... 2004, 304 :17 97 -18 00 Sako Y, Minoghchi S, Yanagida T: Single-molecule imaging of EGFR signalling on the surface of living cells Nat Cell Biol 2000, 2 :16 8 -17 2 Dickson RM, Norris DJ, Tzeng YL, Moerner ... Acad Sci USA 2004, 10 1 :15 9 21- 15926 Yu J, Xiao J, Ren X, Lao K, Xie XS: Probing gene expression in live cells, one protein molecule at a time Science 2006, 311 :16 00 -16 03 Nagai T, Ibata K, Park...
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Tài liệu Báo cáo khoa học: Kinetics of dextran-independent a-(1 fi 3)-glucan synthesis by Streptococcus sobrinus glucosyltransferase I pdf
... of the nigerooligosaccharide acceptor reaction by using GBd-deficient GTF-I (GS) and GTF-I containing a full-length GBd (GSGB) from Streptococcus sobrinus 6 715 (serotype g) (Fig 1) A numerical ... 70 ± 27 ± 26 ± 16 ± ND 5± Acc kcat ⁄ Km (s )1 mM )1) 31 kcat (s )1) 0.56 2.0 2.2 4.6 41 41 69 65 59 40 ± ± ± ± 0.27 0.4 0.3 1. 2 11 ± ± ± ± ± ± ± Acc Km (mM) 25 12 19 78 12 33 17 13 ± ± ± ± ± ± Acc ... The following supplementary material is available: Fig S1 13 C-NMR analysis of the insoluble glucan produced by GS and GSGB Fig S2 TLC analysis of saccharide production by GS and GSGB Fig S3 Double...
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Tài liệu Báo cáo khoa học: Enzymatic and electron paramagnetic resonance studies of anabolic pyruvate synthesis by pyruvate: ferredoxin oxidoreductase from Hydrogenobacter thermophilus doc
... 256, 11 137 11 144 11 Pieulle L, Guigliarelli B, Asso M, Dole F, Bernadac A & Hatchikian EC (19 95) Isolation and characterization of the pyruvate-ferredoxin oxidoreductase from the sulfate-reducing ... nonsaturating conditions.) Although the [3Fe-4S ]1+ signal in Fig 3A disappeared at temperatures exceeding 30 °K, the g = 2.0040 signal remained even at 70 °K (data not shown), indicating that this signal ... acetylCoA and CO2, by the coupled assay with OGOR and LDH (Fig 1) Although carboxylation activity is generally determined by monitoring the incorporation of 14 CO2 to form [14 C] pyruvate, OGOR catalyzes...
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Tài liệu Báo cáo khoa học: Interferon-a induces sensitization of cells to inhibition of protein synthesis by tumour necrosis factor-related apoptosis-inducing ligand ppt
... 720 18 0 360 540 720 +TRAIL (4h) Sub -G1 1. 6% 0 200 400 600 200 DNA content 600 Ce ll c ount Sub -G1 6 .1% + IFNα +TRAIL (16 h) 18 0 360 540 720 +TRAIL (16 h) 18 0 360 540 720 400 DNA content Sub -G1 16 .0% ... x 10 -3 B 75 p 41/ 43 50 (Pro)caspase-8 25 0 .1 10 10 0 TRAIL (ng/ml) (log scale) p18 α-tubulin C TRAIL Control TRAIL + z-IETD.FMK z-IETD.FMK full length 0 0 .1 0.5 1. 0 5.0 10 25 BID t-BID TRAIL (ng/ml) ... Res 21, 3733–3738 17 Fulda S & Debatin KM (2002) IFNgamma sensitizes for apoptosis by upregulating caspase-8 expression through the Stat1 pathway Oncogene 21, 2295–2308 18 Suk K, Kim YH, Chang I,...
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Tài liệu Báo cáo khoa học: Stimulation of poly(A) synthesis by Escherichia coli poly(A)polymerase I is correlated with Hfq binding to poly(A) tails ppt
... 5¢-GAAAAAAGGGGCCACTCAGG 5¢-T (18 )GAAAAAAGGGGCCACTCAGG 5¢GCGTCGCTAATTCTTGCGAGA18TTTCAGAAAAGGGCTG 5¢-C (18 )GAAAAAAGGGGCCACTCAGG 5¢ -G( 18 )GAAAAAAGGGGCCACTCAGG 5¢-GAATTGCTGCCGTCAGCTTGA 5¢-TAATTAACCCTCACTAAAGGGAAACGGAGCGGCACCTCTT ... 5¢-TAATTAACCCTCACTAAAGGGGTGCTCGGCATAAG 5¢-GCCATGAATATCTCCAACGAG 5¢-CATCCAAAATACGCCATGAATATC 5¢-TAATACGACTCACTATAGGGGCCGCTTAACGTCGCG 5¢-GCTTCAGTACTTAGAGAC 5¢-TAATACGACTCACTATAGGGAGACGTAGCACGTTACACC 5¢-GAAAAAAGGGGCCACTCAGG ... constructed as follows A PCR fragment was generated using reverse and forward primers with the sequences 5¢-GGGAATTCCATATGGCTAAGGGGCAA TC and 5¢-AGGATCGCTGGATCCCCGTGTAAAAAA AC, respectively, with...
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Tài liệu Báo cáo Y học: Dinucleoside polyphosphates stimulate the primer independent synthesis of poly(A) catalyzed by yeast poly(A) polymerase ppt
... (08/00 21. 1/20 01) H.O was supported by a Fellowship from Fundacao para a Ciencia e a Tecnologia (SFRH/BD/ ¸ ˜ ˆ 14 77/2000) 10 11 12 13 14 15 16 17 18 19 20 REFERENCES Sillero, M.A .G. , Socorro, S., Baptista, ... mM EDTA, 10 0 lgÆmL )1 acetylated BSA, 10 % glycerol, 0.5 mM MgCl2, 0.2 mM ATP and 0.44 units of the enzyme (part A) Reaction mixtures supplemented with 0.04 mM Gp 2G, 0 .1 mM Gp 3G or Gp 4G are shown ... According to our results, the amount of Ó FEBS 2002 Poly(A) polymerase activation by dinucleotides (Eur J Biochem 269) 5327 Fig Effect of diguanosine polyphosphates (Gp 2G, Gp 3G, Gp 4G, Gp 5G) on the...
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Tài liệu Báo cáo Y học: Phosphatidylinositol synthesis and exchange of the inositol head are catalysed by the single phosphatidylinositol synthase 1 from Arabidopsis docx
... CDP-DAG Reverse reaction Lower phase Upper phase Exchange reaction – CMP + lM CMP –PIS 2 019 ± 71 )10 8 ± 10 3 ± 13 )1 125 ± 11 09 ± 13 8 0 exogenous PtdIns De novo synthesis was measured in the incubation ... the C16:0/C17c + C18 :1/ C17c and C16:0/C19c peaks are characteristic of E coli (A.-M Justin, unpublished data) whereas the C16:0/C18:2 and C18:0/C18:2 PtdIns are characteristic of soybean [14 ] ... (Fig 2A), with values close to 0.8 nmol PtdInsÆmg )1 min )1 at mM EDTA, increasing to nmol PtdInsÆmg )1 min )1 between 2.5 and mM EDTA and a drop to at 10 mM Abolition of all exchange activity at 10 ...
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Báo cáo khoa học: Heterologous synthesis of cytochrome c¢ by Escherichia coli is not dependent on the System I cytochrome c biogenesis machinery ppt
... YTTMAWNMGKIKAMVVDGTMPFSQTQVSAAANVIAAIANSGMGALYSPDTLGVVGFKKSR * ** * * YEFMGWNMGKIKA-NLEGE YNAAQVEAAANVIAAIANSGMGALYGPGTDKNVGDVKTR LTLMASHFGRMTP-VVKGQAPYDAAQIKANVEVLKTLSAL-PWAAFGPGTEGG-D FSLVALEFGPLAAM-AKGEMPYDAAAAKAHASDLVTLTKYDPSDLYAPGTSAD-DVKGTA ... Journal 278 (2 011 ) 23 41 2348 ª 2 011 The Authors Journal compilation ª 2 011 FEBS 2347 Biogenesis of cytochrome c¢ 10 11 12 13 14 15 16 17 18 19 H Inoue et al requires coexpression of cmm genes in heme-rich ... FSLVALEFGPLAAM-AKGEMPYDAAAAKAHASDLVTLTKYDPSDLYAPGTSAD-DVKGTA 80 90 10 0 11 0 12 0 13 0 LKENFFQEQDEVRKIATNFVEQANKLAEVAAMGDKDEIKAQFGEVGKACKACHEKFREEE * * * * VKPEFFQNMEDVGKIAREFVGAANTLAEVAATGEAEAVKTAFGDVGAACKSCHEKYRAKARPEIWSDAASFKQKQQAFQDNIVKLSAAADAGDLDKLRAAFGDVGASCKACHDAYRKKK...
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Báo cáo khoa học: Iron regulatory protein-independent regulation of ferritin synthesis by nitrogen monoxide pot
... containing 10 % fetal bovine serum, extra l-glutamine (300 lgÆmL )1) , sodium pyruvate (11 0 lgÆmL )1) , penicillin (10 0 unitsÆmL )1) , and streptomycin (10 0 lgÆmL )1) Gel-retardation assay The gel-retardation ... 7.5, 14 0 mm NaCl, 1. 5 mm MgCl2, 10 mm dithiothreitol, 10 0 lgÆmL )1 cycloheximide, and 0.5 mgÆmL )1 heparin) and centrifuged in a SW41Ti rotor (Beckman, Palo Alto, CA) (17 8 305 g, 12 0 min, °C) without ... Ft synthesis A 10 0μM SNP A 15 30 1. 0 35S M Mikhael et al 60 1. 4 3.2 12 0 18 0 (min) H+L 7 .1 15.7 24.8 (D.A.) 10 0μM SNP B B 15 30 60 12 0 18 0 (min) IRP IRP IRP +β-ME IRP Fig Effects of SNP on ferritin...
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Báo cáo khoa học: 15 N-Labelled proteins by cell-free protein synthesis Strategies for high-throughput NMR studies of proteins and protein–ligand complexes doc
... (2006) 415 4– 415 9 ª 2006 The Authors Journal compilation ª 2006 FEBS 15 K Ozawa et al 10 11 12 13 14 15 16 17 Nureki O & Yokoyama S (20 01) Selenomethionine incorporation into a protein by cell-free ... 272, 316 2– 317 1 Wu PSC, Ozawa K, Jergic S, Su XC, Dixon NE & Otting G (2006) Amino-acid type identification in 15 N-HSQC spectra by combinatorial selective 15 N-labelling J Biomol NMR 34, 13 – 21 Ozawa ... S30 cell extract [7 ,19 ] or by replacing the originally recommended glutamate buffer [1] by acetate [7 ,18 ] Different amino acids are susceptible to [15 N]-scrambling in the wheat germ system than...
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Báo cáo khoa học: Induction of PPARb and prostacyclin (PGI2) synthesis by Raf signaling: failure of PGI2 to activate PPARb potx
... 5¢—AGGGGGTGCCAGTGATAGAG—3¢; PPARb forward, 5¢—AAGAGGAGAAAGAGGAAG TGG—3¢; PPARb reverse, 5¢—ATTGAGGAAGAGGCTG CTGA—3¢; actin forward, 5¢—GATGATGATATCGCCGC GCTCGTCGTC—3¢; actin reverse, 5¢—GTGCCTCAGGG ... TTACTGTTGTGGTTCTA—3¢; cPLA2 reverse, 5¢—AGT GTCTCGTTCGCTTCC—3¢; COX-2 forward, 5¢—CCATG GGTGTGAAGGGAAATAA—3¢; COX-2 reverse, 5¢—TTG AAAAACTGATGGGTGAAG—3¢; mPGES -1 forward, 5¢—GGTGGCCCAGGAAGGAGACAGC—3¢; ... 5¢—AGAGGGA AATCGTGCGTGAC—3¢; actin reverse, 5¢—CAATAGTG ATGACCTGGCCGT—3¢; PPARb forward, 5¢—GTCGCA CAACGCTATCC—3¢; PPARb reverse, 5¢—CTCCGGGCC TTCTTTTTGGTCA—3¢; cPLA2 forward, 5¢—CATAAGT TTACTGTTGTGGTTCTA—3¢;...
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Báo cáo khoa học: Characterization of the tRNA and ribosome-dependent pppGpp-synthesis by recombinant stringent factor from Escherichia coli pot
... the pET24b vector to generate pET24b(relA) The correct sequence of relA was confirmed by sequencing using the primers 5¢-AGCAATACGCTCCGCCAG-3¢, 5¢-TGGCGGATGCCAACGTAG-3¢, 5¢-CTCGACCGCGA ACACTAC-3¢, ... MA, USA) Cloning of the E coli relA gene The relA gene was amplified by PCR from E coli MRE 600 genomic DNA with primers 5¢-CGGGAATTCCATATGGT TGCGGTAAGAAT-3¢ and 5¢-CCCGCTCGAGACTCCCG FEBS Journal ... primers were used: 5¢-CCGAACTGTCT CACGAC-3¢ (906, 16 S rRNA), 5¢-TGTTATCCCCGGAG TAC-3¢ (2437, 23S rRNA), 5¢-GCATTTCACCGCTACAC-3¢ (683, 16 S rRNA) and 5¢-TCCGTCTTGCCGCGGGT-3¢ (2042, 23S rRNA) The...
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Báo cáo khoa học: Oligosaccharide synthesis in Fibrobacter succinogenes S85 and its modulation by the substrate potx
... MD1Pa, Glc1P unit of MD1P; MD1Pb, Glc unit neighbouring the Glc1P of MD1P; MD, linear maltodextrins; MDt, the terminal Glc unit of MD; MDint, internal Glc units of MD and MD1P X, unknown Glc1P ... Sugar Succinate Acetate Sugar Succinate Acetate No Glc CB M2 M3 M4 M6 M2M3 M2M4 M3M4 M3M6 0.0 12 .6 13 .6 0.0 0.9 0.5 0.5 1. 4 1. 5 1. 0 1. 3 0.0 6.4 6.5 0.0 1. 5 1. 4 2 .1 2.9 3.0 2 .1 2.0 M2 to CB M3 to CB ... Futile cycling of glycogen in Fibrobacter succinogenes as shown by in situ 1H-NMR and 13 C-NMR investigation Eur J Biochem 207, 15 5 16 2 Aghajanian SA, Martin SR & Engel PC (19 95) Ureainduced inactivation...
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Báo cáo Y học: Inhibition of hyaluronan synthesis in Streptococcus equi FM100 by 4-methylumbelliferone doc
... (mM) 1. 0 2.0 Peak number Average % SEM Average % SEM Average % SEM A B C D E F G H I 4.52 6.52 9.59 14 . 61 20.59 18 .78 9.56 7. 91 7.92 0.72 0.43 0. 51 0.20 1. 50 1. 38 0.86 0.28 0.97 3.36 4.85 7.74 11 .27 ... 7.74 11 .27 20.02 26.37 12 .44 7.64 6.33 0.22 0.83 0.98 0.28 0.98 0.99 0 .18 0.50 0. 41 3 .12 3. 41 6.04 10 .03 18 . 91 29.30 14 .39 8.06 6.65 1. 48 0.52 0.67 0.84 1. 45 1. 09 0 .14 0.88 1. 00 added in the cell-free ... after digestion with lysozyme and bovine testicular hyaluronidase The primer set used in the PCR had the following sequence: forward, 5¢-ACTGTTGTGGCCTTTAGTA-3¢ and reverse, 5¢-AAGGGCTGTAGGACAAACAA-3¢...
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Báo cáo Y học: Concerted regulation of free arachidonic acid and hormone-induced steroid synthesis by acyl-CoA thioesterases and acyl-CoA synthetases in adrenal cells pdf
... 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 intramitochondrial cholesterol translocator J Biol Chem 262, 918 1– 918 8 Miller, W.L (19 88) Gene conversions, deletions and polymorphisms in congenital ... although the role of CTE-I in steroidogenesis is currently not known The high degree of sequence similarity between the CTE-I and MTE-I genes suggests that they diverged relatively recently by gene ... adding 0 .1 mM isopropyl thio-b-D-galactoside (IPTG) After recovery by centrifugation, cells were resuspended and lysed by sonication (4 · 15 s) in NaCl/Pi, containing 0 .1% 2-mercaptoethanol, mgÆmL)1...
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Báo cáo khoa học: DNA mediated disassembly of hRad51 and hRad52 proteins and recruitment of hRad51 to ssDNA by hRad52 pot
... light scattering The sequence of the 12 1-mer ssDNA substrate PUC+, used in this study was: 5¢—TTTCCCAGTCACGA CGTTGTAAAACGACGGCCAGTGCCAAGCTTGCAT GCCTGCAGGTCGACTCTAGAGGATCCCCGGGTAC CGAGCTCGAATTCGTAATCATGGTCATAGCTGTTT ... analysed by native gel electrophoresis (Fig 1A) and centrifugation assays (Fig 1B) hRad 51 migrated as a highly aggregated form (several hundred kDa complexes) that barely entered into the gel Only ... al DNA-induced disassembly of higher oligomeric forms of hRad52 Protein aggregation/disaggregation changes vs ionic effects A similar effect of disaggregation by ssDNA was also observed with...
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Báo cáo khoa học: Methylene analogues of adenosine 5¢-tetraphosphate Their chemical synthesis and recognition by human and plant mononucleoside tetraphosphatases and dinucleoside tetraphosphatases pot
... 92, 218 1– 218 2 46 Dixon M & Webb EC (19 64) Enzymes, 2nd edn Academic Press, New York 47 Blackburn GM (19 81) Phosphonates as analogues of biological phosphates Chem Industry (London) 13 4– 13 8 48 ... Physiol 13 1, 14 01 14 10 Madrid O, Martı´ n D, Atencia EA, Sillero A & Gunther ¨ Sillero MA (19 98) T4 DNA ligase synthesizes dinucleoside polyphosphates FEBS Lett 433, 283–286 Atencia EA, Madrid O, Gunther ... Ap4A and Other Dinu- 836 15 16 17 18 19 cleoside Polyphosphates (McLennan AG, ed.), pp 29– 61 CRC Press, Boca Raton, FL Ogilvie A & Jacob P (19 83) Diadenosine 5¢,5¢¢¢-P1,P3triphosphate in eucaryotic...
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Tìm thêm: xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25