with reduced enzymatic activity
Báo cáo y học: "Serum cholesterol concentration associated with aspirin esterase activity in older people: preliminary data"
... population did not include subjects with malnutrition nor there were any with nutritional modifications during the interventional period. More studies with larger sample sizes and longer ... height measured in light indoor clothing without shoes. Serum total cholesterol and plasma glucose were enzymatically determined. Serum aspirin esterase activity was measured kinetically by a ... serum total cholesterol concentrations are clearly associated with those of aspirin esterase activ-ity during a period of physical activity modification in an older population. This finding suggests...
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Tài liệu Báo cáo khoa học: A novel dicyclodextrinyl diselenide compound with glutathione peroxidase activity ppt
... displays GPX activity FEBS Journal 274 (2007) 3846–3854 ª 2007 The Authors Journal compilation ª 2007 FEBS 3847A novel dicyclodextrinyl diselenide compound with glutathione peroxidase activity Shao-Wu ... theGPX-like activity assay. Activity was determined by thedecrease of NADPH absorption at 340 nm (eNADPH¼6220 m)1cm)1). Background absorption of the noncatalyticreaction was run without ... compounds with gluta-thione peroxidase activity. J Am Chem Soc 111, 5936–5939.12 Iwaoka M & Tomoda S (1994) A model study on theeffect of an amino group on the antioxidant activity ofglutathione...
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Tài liệu Báo cáo khóa học: New activities of a catalytic antibody with a peroxidase activity ppt
... formation is lower in the presence of theantibody, with a decrease in the rate constant of 7% beingobserved with hydroxylamine 1 andof>40%withthemore bulky hydroxylamine 2.The amount of ... Tresca, J.P., Ricoux, R., Pontet, M. & Engler, R. (1995) Com-parative activity of peroxidase–antibody conjugates with periodateand glutaraldehyde coupling according to an enzyme immuno-assay. ... & Pratt, J.M. (1987) Hemes andhemoproteins. 3. The reaction of microperoxidase-8 with cyanide:comparison with aquocobalamin and hemoproteins. J. Inorg.Biochem. 29, 77–91.23. Dai, L. &...
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Tài liệu Báo cáo khoa học: A selenium-containing single-chain abzyme with potent antioxidant activity docx
... CCO activity. Over 60 min, CCO activity in the damage groupdecreased from 0.356 ± 0.012 to 0.208 ± 0.010 U per mgprotein, i.e. by % 41.6%. Figure 3 shows that CCOprotection increased with ... liver.Arch. Biochem. Biophys. 340, 59–63.Ó FEBS 2003 Se-containing abzyme with potent antioxidant activity (Eur. J. Biochem. 270) 4331 with oxidation of cytochrome c in the sample cell, intowhich 5 lL10mMK3Fe(CN)6was ... protect themselves from oxidative injury, aerobiccells have evolved an enzymatic and nonenzymatic defensesystem. The enzymatic antioxidant system is mainlycomposed of glutathione peroxidase...
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Tài liệu Báo cáo khoa học: The molecular surface of proteolytic enzymes has an important role in stability of the enzymatic activity in extraordinary environments pptx
... enzymes with high alkalinity.Several mutant enzymes showed drastically improvedstability in solutions containing SDS (Fig. 4). ALP Imaintained 60% of enzymatic activity after 1 h, and 20%of activity ... (3.8 · 25 cm) equili-brated with the same buffer. The enzyme active fraction waseluted with a 0–1.0MNaCl linear gradient and pooled. Theactive fraction was dialysed with 10 mMMes at pH 6.5containing ... enzymes. They lost the activity within 10 min as didwild-type ALP I.Resistance to surfactantsResidual activities of the mutant enzymes were measuredafter incubation with 0.1% SDS in 0.1MH3BO3/Na2CO3/KCl...
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Tài liệu Báo cáo Y học: Analyses of the CYP11B gene family in the guinea pig suggest the existence of a primordial CYP11B gene with aldosterone synthase activity docx
... incubated for 48 h with 5 lM11-deoxy-corticosterone (DOC) including 4 nCiÆmL)1[14C]DOC. Metaboliteswere analysed by TLC. Enzymatic activity is given as relative radio- activity. White bars ... combi-nation with [a-35S]dCTP followed by autoradiography with HyperfilmTMMP (Amersham).Southern blottingGenomic DNA was digested with the appropriate enzymes,extracted twice with phenol/chloroform ... synthesized by CYP11B2 in comparison with CYP11B1 (Fig. 4C). It is noteworthy, that CYP11B2displayed a higher enzymatic activity than CYP11B1 basedon 11b-hydroxylase activity. These differences were...
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Báo cáo khóa học: A new UV-B absorbing mycosporine with photo protective activity from the lichenized ascomycete Collema cristatum docx
... A new UV-B absorbing mycosporine with photo protective activity from the lichenized ascomyceteCollema cristatumAvital Torres1, Malka Hochberg4, ... isocratic elution with 60% EtOH – 40%MeOH, to yield 50 mg of a pale yellow powder. Purity ofthe isolated compound was confirmed by HPLC analysis,which showed a single sharp peak with a retention ... supplemented with 10% fetal bovine serum,L-glutamine 2 mMand antibiotics (100 U penicillin permL and 100 mg streptomycin per mL). Cells were grown to80% confluence in 9 cm culture dishes, overlaid with...
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Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx
... low levelof NAAG-hydrolyzing activity, < 0.01% of the 44/750variant, was associated with the 44/750_V5-His variant, andno proteolytic activity could be detected with variantsN-terminally ... weremixed with an equal volume of the denaturing SDS buffer and 10 lLof the mixture was loaded onto a single lane. Activity levels are indi-cated as follows: (+), measurable NAAG-hydrolyzing activity; ... catalytic activity, such as aminopeptidasesfrom Streptomyces griseus and Vibrio proteolyticus [13].Additionally, the homology of human GCPII with thetransferrin receptor has been reported, with...
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Báo cáo khoa học: G protein-coupled receptor 30 down-regulates cofactor expression and interferes with the transcriptional activity of glucocorticoid pdf
... transfected with the expression vectors encoding GRa and GPR30 (or with the corresponding control vectors) together with thereporter construct MMTV-tk-luc. MMTV-tk-luc promoter activity was ... cells, theeffect of hydrocortisone on MMTV-luc activity w as almostabrogated. Without the presence of hydrocortison, GPR30had no effect on the activity, indicating that the effect isglucocorticoid-dependent. ... treatmentin control cells (Fig. 5B). When the cells were transfected with GPR30 and treated with dexamethasone, the luciferase activity was almost abolished. GPR30 did not affect thetarget promoter...
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Báo cáo khoa học: Peptides that bind the HIV-1 integrase and modulate its enzymatic activity – kinetic studies and mode of action pptx
... 150. IN enzymatic activity was measured as described previously[58] and in Experimental procedures (t-test, P < 0.01). A–I with the different peptide as indicated in the figure, K without ... (Sigma), as described previously [79].The enzymatic activity of horseradish peroxidase wasestimated by monitoring the product’s absorbance at490 nm with an ELISA plate reader (Tecan Sunrise,Ma¨nnedorf, ... indicates that theobserved peptide activity cannot be attributed solely tothe masking of a specific sequence or domain withinIN. In addition, the three peptide groups, with thedifferent kinetic profiles...
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Báo cáo khoa học: The specificity of alcohol dehydrogenase with cis-retinoids Activity with 11-cis-retinol and localization in retina docx
... with 13-cis-retinoids, although with low kcatvalues (Tables 2 and3). Human ADH1 enzymes were not found to be active with 13-cis-retinoids, although a low activity had been previouslyreported with 13-cis-retinal ... bindingpocket of ADH4 is the reason for the low activity observed with this retinal isomer.The interaction of 11-cis-retinal with ADH1B1 was alsostudied and compared with that of ADH4 (Fig. 2A–D).11-cis-retinal ... by immunohistochem-istry, consistent with the ADH4 activity previously found inthis epithelium [25]. Thus, the presence in the RPE, the high activity with 11-cis-retinol, and the specificity...
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Tìm thêm: xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25