who was responsible for the creation of christianity
Tài liệu Báo cáo khoa học: A novel coupled enzyme assay reveals an enzyme responsible for the deamination of a chemically unstable intermediate in the metabolic pathway of 4-amino-3-hydroxybenzoic acid inBordetellasp. strain 10d doc
Ngày tải lên :
19/02/2014, 16:20
... in cell extracts of succinate/glucose-grown cells;
therefore, the s ynthesis of the e nzyme was indu cible. Table 1
shows a summary of a typical enzyme purification. The
enzyme was purified 103-fold ... the
determination of released ammonia. The coupled enzyme
assay revealed the mechanism of the deamination reaction
and the subsequent metabolism, including the deamination
step.
The product formed ... to clarify
the stoichiometry by adding a small amount of the purified
dioxygenase to the reaction mixture with a large excess of
the purified enzyme reported here to avoid the formation
of 2,5-pyridinedicarboxylic...
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Tài liệu Báo cáo Y học: Interallelic recombination is probably responsible for the occurrence of a new as1-casein variant found in the goat species potx
Ngày tải lên :
22/02/2014, 04:20
... 37% B was applied a t a flow rate of
0.5 m Lặmin
)1
over 60 min. The column efuent was split
1 : 25 to give a ow rate of % 4 lLặmin
)1
into the
electrospray nebulizer. The bulk of the flow was run
throughthedetectorforpeakcollectionasmeasuredby
following ... phosphorylation of Ser64 in the M variant. The
sequence determined is consistent with the molecular mass
measured for the native protein. The phosphorylated
residues a re therefore Ser46, 48, ... recombination at the a
s1
-casein locus (Eur. J. Biochem. 269) 1303
skipping of three successive exons during the course of the
processing of the primary transcripts [9]. At the peptide
level, the CfiT...
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Báo cáo khoa học: Identification of the structural determinant responsible for the phosphorylation of G-protein activated potassium channel 1 by cAMP-dependent protein kinase pdf
Ngày tải lên :
07/03/2014, 00:20
... distal
part is unique for GIRK1 among the Kir3.x isoforms.
Further truncations of the C-terminus revealed a 49
amino acid stretch (amino acids 362–411) that was the
most prominent target for phosphorylation ... by the catalytic subunit of PKA. In the lower panels, the mean values and
standard error of mean values of relative specific radioactivity of the different constructs are plotted. The number of ... previously
[18]. To assess the role of the S ⁄ Ts in the regulation of
GIRK1 via PKA in a manner that is unbiased by the
eventual contributions of the other subunits in a het-
erooligomer, the corresponding...
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Báo cáo khoa học: Direct CIII–HflB interaction is responsible for the inhibition of the HflB (FtsH)-mediated proteolysis of Escherichia coli r32 by kCIII docx
Ngày tải lên :
23/03/2014, 10:20
... general mode
of CIII activity, or does it apply only for CII? We
examined the mode of the inhibitory action of CIII on
proteolysis of the heat shock sigma factor r
32
, another
substrate of HflB. Like ... compared with 40% for the control
(r
32
alone, Fig. 2C).
The inhibitory action of CIIIC on the proteolysis of
r
32
by HflB was also assayed in the presence of CIIIC
(60 lm) instead of CIII (Fig. 2B). ... mode of action for kCIII would be independent of the HflB substrate. In
this study, we tested the ability of CIII to protect r
32
from HflB digestion.
The inhibition of HflB-mediated proteolysis of...
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Báo cáo khoa học: Two short protein domains are responsible for the nuclear localization of the mouse spermine oxidase l isoform pdf
Ngày tải lên :
07/03/2014, 17:20
... standard
techniques [12]. The absence of errors in DNA products
generated by the PCR was verified by sequence analysis.
The deletion mutant of the mSMOl protein was con-
structed by the PCR following the method ... is
localized on the tip of the FAD-binding domain, in
close spatial proximity to the protein region encoded
by exon VIa of the mSMOl isoform. This observation
has led us to hypothesize that these two ... numbering of the human
SMO enzyme). The two protein domains may have
coevolved, conferring novel properties to mammalian
SMOs.
Molecular modeling of the 3D structure of the
mSMOl isoform was thus...
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Báo cáo khoa học: A characteristic Glu17 residue of pig carnitine palmitoyltransferase 1 is responsible for the low Km for carnitine and the low sensitivity to malonyl-CoA inhibition of the enzyme docx
Ngày tải lên :
16/03/2014, 04:20
... expression of the liver and muscle
isoforms of ovine carnitine palmitoyltransferase 1: resi-
dues within the N-terminus of the muscle isoform influ-
ence the kinetic properties of the enzyme. ... ascer-
tain whether the presence of a negative domain in the
N-terminal region of the pig enzyme could be responsi-
ble for its low level of malonyl-CoA inhibition, we
determined the IC
50
of wild-type ... that the N-terminal fragment of
pig or human CPT1B enzymes determines the overall
malonyl-CoA sensitivity of these enzymes. Thus, the
N-terminal 50 amino acids of the human sequence
increased the...
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Báo cáo khoa học: A novel factor XI missense mutation (Val371Ile) in the activation loop is responsible for a case of mild type II factor XI deficiency doc
Ngày tải lên :
23/03/2014, 07:20
... important for stabi-
lizing the active state (e.g. Asp189, which is part of the
S1 pocket responsible for the binding specificity of the
substrate) [26]. Consequently, substitution of Val371 to
isoleucine ... explained by the proximity of the
mutation to the cleavage site, probably resulting in a
small interference with the binding of the activator to
the FXI zymogen.
There are some examples of inherited ... consequences of the Val371Ile substitution. Rib-
bon representation of the superimposition between the structures
of the catalytic serine protease domain of the zymogen (red) and
activated (green) FXI. The...
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Báo cáo khoa học: PSI1 is responsible for the stearic acid enrichment that is characteristic of phosphatidylinositol in yeast pdf
Ngày tải lên :
29/03/2014, 22:21
... cen-
trifugation, the organic phase was isolated and the remain-
ing lipids were further extracted by the addition of 2 mL of
chloroform to the aqueous phase and by shaking (in the
presence of the glass ... in the
de novo synthesis of a given phospholipid, including
PI. Hence, the rate of synthesis and the amount of
CDP-DAG molecules that were used as substrate for
PI synthesis appeared to be the ... yeast. The first hypothesis, previously raised for
plant cells [33], involves the synthesis of two kinds of
CDP-DAG molecules: the first type containing stearic
acid at the sn-1 position would be the...
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Báo cáo y học: " Derivation and preliminary validation of an administrative claims-based algorithm for the effectiveness of medications for rheumatoid arthritis"
Ngày tải lên :
25/10/2012, 10:45
... on the prevalence in the
population, and more reflective of the test itself, lessening the impact of any unique features of
the VA population. Moreover, we might expect that the PPV and NPV of ... VARA visit on the same day or within 1 month of biologic initiation.
The date of initiation of the biologic defined the start of a one year ‘treatment episode’, which
began on the ‘index date’. ... claims
data.
To test the performance of the effectiveness algorithm and to see whether it was similar for
non-biologic RA treatments, we performed a separate analysis of RA patients enrolled...
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Báo cáo y học: "Clinical Strategy for the Management of Solid Pseudopapillary Tumor of the Pancreas: Aggressive or Less"
Ngày tải lên :
25/10/2012, 11:40
... (SPN),
the experience with the management of disseminated
disease is very limited in the literature
6
, therefore,
attention to the therapy strategy of this neoplasm in
the future may allow a ... 1 male with a median
age of 23 years. The median diameter of the lesions was 8.0 cm. Abdominal pain was the
predominant complaint (8/18). The rest of the patients were asymptomatic and presented ... result in the uncertainty of the
strategy for its treatment. While further delineati n g
the clinicopathological and radiological features, and
summarizing the surgical therapy strategy of this...
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