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Báo cáo khoa học: A characteristic Glu17 residue of pig carnitine palmitoyltransferase 1 is responsible for the low Km for carnitine and the low sensitivity to malonyl-CoA inhibition of the enzyme docx
... of carnitine: mm (for standard comparison with other published data), and a concentration equal to the Km for carnitine of each enzyme (for comparison between mutants) In this article, we show ... sequence as being responsible for the peculiar kinetic characteristics of this enzyme, acting as a negative determinant for malonyl-CoA sensitivity Asp17 may account, at least in part, for the high ... been dissected in the N-terminal region of this enzyme, which is less malonyl-CoA sensitive than human CPT1B The IC50 for malonyl-CoA inhibition of human CPT1B (IC50 = 0.096 lm) [7,22,23] is ...
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Báo cáo khoa học: A novel factor XI missense mutation (Val371Ile) in the activation loop is responsible for a case of mild type II factor XI deficiency doc
... the active conformation, Val371 forms contacts with neighboring residues that are important for stabilizing the active state (e.g Asp189, which is part of the S1 pocket responsible for the binding ... sequence identity of 3045%), this position is occupied solely by a valine This conserved amino acid is replaced by isoleucine in the Val371Ile FXI mutant Interestingly, an isoleucine residue naturally ... this position most likely causes some degree of structural change; this is especially true in the case of the introduction of an isoleucine, a b-branched amino acid that is not exible In the active...
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Báo cáo khoa học: Direct CIII–HflB interaction is responsible for the inhibition of the HflB (FtsH)-mediated proteolysis of Escherichia coli r32 by kCIII docx
... However, this proteolysis is much slower in vitro, because of a lack of chaperone machinery The in vitro proteolysis requires ATP [14] We examined the proteolysis of purified r32-C-his (1.2 lm) ... proteolysis of r32 by different concentrations of CIII or CIIIC The bands corresponding to r32-Chis (1.2 lM) remaining after proteolysis with GST–HflB (0.8 lM) for 40 in the presence of His6–CIII ... CIII on proteolysis of the heat shock sigma factor r32, another substrate of HflB Like proteolysis of CII, r32 proteolysis requires ATP Both CIII and CIIIC inhibit this proteolysis, with CIIIC exhibiting...
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Báo cáo khoa học: PSI1 is responsible for the stearic acid enrichment that is characteristic of phosphatidylinositol in yeast pdf
... synthesis, it might be involved in the stearic acid incorporation after the de novo synthesis of this lipid (i.e the second hypothesis) According to this hypothesis, previously raised for mammals ... regulation [1–5] The fatty acid composition of this phospholipid is quite distinctive in living organisms because, in comparison with other phospholipids, PI is by far the most saturated In bovine heart, ... from the European S cerevisiae Archive for Functional Analysis 6420 (EUROSCARF) library BY4742 (MATa; his3D1; leu2D0; lys2D0; ura3D0) is a wild-type strain and psi1D (MATa; his3D1; leu2D0; lys2D0;...
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Báo cáo khoa học: Redox reaction between amino-(3,4-dihydroxyphenyl)methyl phosphonic acid and dopaquinone is responsible for the apparent inhibitory effect on tyrosinase doc
... o-quinone) the values obtained are not very precise (150 mV for Dopa and 195 mV for compound 1), but indicate that the formation of the phosphonic o-quinone is not favored Thus, two other factors drive ... solution What distinguishes our case from other redox 4104 B Gasowska et al (Eur J Biochem 269) ˛ interactions in this system is the decomposition of the phosphonic o-quinone This decomposition ... mushroom (Agaricus bisporus) tyrosinase isoforms by tropolone J Agric Food Chem 47, 2638–2644 Kahn, V & Andrawis, A (1985) Inhibition of mushroom tyrosinase by tropolone Phytochemistry 24, 905–908...
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Báo cáo y học: " JNK activation is responsible for mucus overproduction in smoke inhalation injury" potx
... contributions WIC was responsible for carrying out the experiments, for data analysis, and for drafted this manuscript; KYK was responsible for the analysis and design for the histologic study; OS ... SPSS (Version 13.0 software) For comparison between groups, analysis of variance(ANOVA) followed by multiple comparisons by Scheffé’s test with Bonferroni post hoc analysis Significance was set at ... twice with distilled water for each The endogenous peroxidase activity was blocked with 3% hydrogen peroxide (H2O2) in PBS for min; the slides were rinsed twice with PBS for Sections for positive...
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Tài liệu Báo cáo khoa học: Is ATP binding responsible for initiating drug translocation by the multidrug transporter ABCG2? docx
... ABCG2R482G remains in a conformation that contains a low-affinity binding site for [3H]daunomycin immediately post-nucleotide hydrolysis Discussion A precise molecular mechanism for substrate translocation ... binding affinity for [a32P]azido-ATP as KD = 201 ± 80 lm This affinity is similar to the value obtained for ATP binding to ABCB1 [29] The ability of nucleotides to displace binding is shown in Fig ... low-affinity conformation of drug binding sites in ABC multidrug efflux pumps is assumed to correspond to the outward-facing conformation The energy produced by the hydrolysis of ATP is harnessed for the...
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Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate specificity docx
... kDa It is assumed that there is one active site per monomer Where cooperativity is observed, the Hill coefficient (n) is given When kcat ⁄ Km is compared with the wild-type, dThd and dCyd is set ... preceding step is rate determining for the overall forward reaction (R Browne, G Andersen, G Le, B MunchPetersen and C Grubmeyer, unpublished results) Therefore, and because ATP is saturating ... Surprisingly, mutation of R105 to K completely abolishes the catalytic efficiency A possible explanation for this is that lysine is more flexible than Arg, and therefore not able to position E52, and catalyse...
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Tài liệu Báo cáo Y học: The insert within the catalytic domain of tripeptidyl-peptidase II is important for the formation of the active complex potx
... surrounding Arg252 is of importance for the formation of the oligomeric enzyme complex, which is a prerequisite for obtaining a fully active enzyme [8,15] Upon removal of this entire region (amino ... characteristics when overexpressed in human 293-cells The main objective of the present work was to find an explanation for this phenomenon It is demonstrated that the formation of the active complex is ... these two clones is the amino acid in position 252 Evidently, changing Gly252 to an Arg was critical for the association properties of the enzyme The nonassociated form is inactive For purified human...
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Tài liệu Báo cáo Y học: Interallelic recombination is probably responsible for the occurrence of a new as1-casein variant found in the goat species potx
... hydrolysis), corresponding to that expected for the F variant This result is the first evidence for the heterozygous status (M/F) of the individual goat milk analysed In addition to this, the ... times: denaturation for 30 s (or min) at 94 °C, annealing for 30 s (or min) at 47–60 °C, and extension for 30–60 s (or min) at 72 °C, Fig Disc-PAGE at pH 8.6 of individual whole caprine casein ... event at the origin of the as1-CasM allele This is, to our knowledge, the first hypothesis of a genomic recombination event to account for genetic polymorphism at a locus encoding a milk protein MATERIALS...
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Báo cáo khoa học: Catalytically active membrane-distal phosphatase domain of receptor protein-tyrosine phosphatase a is required for Src activation doc
... here, for the first time, that a functional, catalytically active D2 is required for RPTPa to bind to its substrate, Src, and to dephosphorylate and activate it Active RPTPa-D2 is required for Src ... We discovered an R554H mutation in the KNRY motif of RPTPa-D2 in a screen for disease-related mutations in RPTPs Whereas this mutation appears not to be linked to disease, we observed that this ... D2-C723S, the inactive mutant (Fig 1) These results show that the Arg554 residue is essential for the catalytic activity of RPTPa-D2 Catalytic activity of the RPTPa D2 domain is required for Src binding...
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Báo cáo khoa học: The b domain is required for Vps4p oligomerization into a functionally active ATPase potx
... This study This study This study This study This study This study This study This study This study This study This study This study This study This study This study This study This study This ... rings Consistent with this, wild-type Vps4p purified from bacteria forms a dimer [24], and Vps4p forms a 10–12-mer when its ATPase activity is compromised and it is locked in the ATP-bound conformation, ... essential for interaction with Vta1p In addition, we show that one of these motifs is required for a homotypic interaction and for formation of a highly active ATPase complex, but is not required for...
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Báo cáo hóa học: "TAp73 is one of the genes responsible for the lack of response to chemotherapy depending on B-Raf mutational status" pot
... TA isoforms act similarly to p53, inducing apoptosis In comparison, ΔN isoforms have little transactivation activity and play a role blocking target genes of p53 and their respective TAp73 isoforms ... their respective TAp73 isoforms [6] Therefore, the TA isoforms may be expected to have functions in tumor suppression while ΔN isoforms might be oncogenic For the first time in 2006, Dominguez et ... curve analysis Western Blot Analysis For protein analysis, 7.5 × 105 cells were seeded, and after treatment, harvested, washed in ml of cold PBS and lysed in EBC lysis buffer (50 mM Tris pH8, 120...
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Báo cáo khoa học: " Cap-independent protein translation is initially responsible for 4-(N-methylnitrosamino)-1-(3-pyridyl)-butanone (NNK)-induced apoptosis in normal human bronchial pithelial cells" pot
... USA) Statistical analysis Results are shown as mean ± SE Statistical analyses were performed following analysis of variance (ANOVA) for multiple comparisons or Students t-test when data consisted ... timedependent study with Annexin V and propidium iodide (PI), which is an important marker for distinguishing early apoptosis and necrosis Untreated cells were not stained with Annexin V and PI, suggesting ... apoptosis, probably reflecting that Apaf-1 is required for initial steps of apoptosis [25] Taken together with above results, our data strongly suggest that IRES-dependent protein translation is responsible...
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Báo cáo y học: "A functional variant of Fcγ receptor IIIA is associated with rheumatoid arthritis in individuals who are positive for anti-glucose-6-phosphate isomerase antibodies" doc
... CG, van de Winkel JG, Tervaert JW: Fcgamma receptor polymorphisms in Wegener's granulomatosis: risk factors for disease relapse Arthritis Rheum 1999, 42:1823-1827 Myhr KM, Raknes G, Nyland H, Vedeler ... used two antigens for the discrimination, the cutoff OD (mean value + standard deviation) was 0.98 for human recombinant GPI and 0.64 for rabbit native GPI Genomic DNA was isolated from 0.5 ml ... the discussion Acknowledgements This work was supported in part by the Japanese Ministry of Science and Culture (IM, TS) IM is also a recipient of a fellowship from the Japan Intractable Diseases...
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Báo cáo y học: "Is disturbed clearance of apoptotic keratinocytes responsible for UVB-induced inflammatory skin lesions in systemic lupus erythematosus" ppsx
... (Figure 4) This group of patients with cutaneous LE lesions could not be distinguished from the patients who got UVB irradiation to the skin by any of the patient characteristics listed in Tables ... apoptotic cells is normal when mice are not in a state of active disease [30,31] Our data provide in vivo evidence that the apoptotic cell clearance rate in patients with inactive SLE is not disturbed ... (or 'butterfly') rash; 2, discoid rash; 3, sensitivity to light, or photosensitivity; 4, oral ulcers; 5, arthritis; 6, serositis; 7, kidney disorder; 8, neurologic disorder; 9, blood abnormalities;...
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Báo cáo y học: "Is disturbed clearance of apoptotic keratinocytes responsible for UVB-induced inflammatory skin lesions in systemic lupus erythematosus" pot
... units Statistical analysis Data are expressed as means ± SEM Statistics were performed with Student's t test, one-way analysis of variance and repeated-measures analysis of variance A comparison was ... Immunohistochemistry staining of p53 protein expression in rheumatoid arthritis FLS Transfected FLS were seeded into four-well chamber slides, cultured for days, and evaluated by immunohistochemistry ... incubated for or days and Western blot analysis was performed The p53 level was decreased on day and day 5, especially with the higher amounts of siRNA For subsequent experiments, 2.5 µg of siRNA for...
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Báo cáo y học: "Fibroblast growth factor receptor 1 is principally responsible for fibroblast growth factor 2-induced catabolic activities in human articular chondrocytes" pptx
... elicit distinctive biological responses FGF-2 binds to all FGFR isoforms, yet with higher affinity to FGFR1 and FGFR3 [32] We investigated which specific FGFR isoforms are responsible for the ... FGFR3 have distinct biological roles in articular chondrocytes, and that activation of FGFR3 by FGF18 is anabolic for human articular cartilage homeostasis [33, 34] FGFR1 is responsible for the upregulation ... Osteoarthritis (OA) is a debilitating disease afflicting millions of people worldwide, which imposes a tremendous burden upon society OA is a multifactorial heterogeneous disease that is influenced...
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Báo cáo y học: "Who is at increased risk for acute kidney injury following noncardiac surger" doc
... surgery, high-risk surgery (defined as intraperitoneal, intrathoracic, or suprainguinal vascular procedures), ischaemic heart disease, congestive heart disease and the Revised Cardiac Risk Index (RCRI) ... some forms of hydroxyethyl starch and so on) to AKI risk Nonetheless, the study makes an important contribution to the effort to define risk factors for AKI after noncardiac surgery It is important ... surgery and ischaemic heart disease, leaving American Society of Anesthesiologists physical status, RCRI score, high-risk surgery and congestive heart failure as the independent preoperative risk factors...
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Báo cáo y học: " Number of active transcription factor binding sites is essential for the Hes7 oscillator" pptx
... protein is crucial for the somite segementation clock Nature Genetics 2004, 36:750-754 Lewis J: Autoinhibition with Transcriptional Delay: A Simple Mechanism for the Zebrafish Somitogenesis Oscillator ... of them active? • Our numerical analysis of the delay system demonstrates that the model is highly sensitive to changes in the Hill coefficient Is this inherent in the Hes7 oscillator or is it ... steepness of (1) is determined by means of a Hill plot For this purpose, log fh(x)/(1 - fh(x)) is plotted against log x for 0.1 ≤ fh(x) ≤ 0.9 The absolute slope of the regression line for the Hill...
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