... minne-
sota. In the presence of EDTA, theoxidative effect of the
rough E. coli LPS approaches that ofthe smooth S. min-
nesota LPS.
The results of experiments, in whichthe oxidation of
Hb A
0
by the four ... measure
of the rate of oxidation of oxyHb.
RESULTS
The effect of pH on the auto-oxidation of cross-linked Hb
was studied. The rate of decrease ofthe concentration of
oxyHb increased as the pH ... rates of oxidation
caused by each ofthe LPSs studied should be similar to each
other, i.e. simply that ofthe rate of oxidation of dimers.
The increase in rate of oxidation in the presence of rough
E....
... forms of the
MAPKs were produced as GST-lofMPK3 and GST-
lofMPK6. However, when lofMPK3 and lofMPK6
proteins were tested for phosphorylation with OXI1,
no phosphorylation of lofMPK3 or lofMPK6 ... pathogen attack.
The Arabidopsis genome encodes 39 AGC kinases,
of which 23 are classified to the AGC VIII group [8,9].
The AGC kinases were named on the basis of their
homology to the mammalian ... front ofthe GUS gene. For PTI1-4, a 1.8 Kb
region upstream ofthe PTI1-4 (At2g47060) translational
start was subcloned EcoRI-XhoI.
The 2.2 Kb OXI1 promoter and the genomic sequence of
OXI1 with the...
... the relationship of these two molecules is still
largely unknown. The aim ofthe current study was to investigate the
potential involvement of nucleolin ⁄ C23 in the antiapoptotic mechanism of
Hsp70. ... and fragmentation of their
nuclei. The percentage of apoptotic cells was calculated as
the number of apoptotic cells divided by the total number
of cells counted. A minimum of 500 cells were ... by
the antisense oligonucleotide, the protection of
Hsp70 is lost.
Taken together, our results demonstrate an impor-
tant relationship among the regulation of nucleo-
lin ⁄ C23, the activation of...
... analysis, and the
determination of released ammonia. The coupled enzyme
assay revealed the mechanism ofthedeamination reaction
and the subsequent metabolism, including the deamination
step.
The product ... [10]. The enzyme catalyzesthe ring fission of
4-amino-3-hydroxybenzoic acid to form 2-amino-5-carb-
oxymuconic 6-semialdehyde (Fig. 1A). The cloning and
nucleotide sequence ofthe gene encoding the ... doi:10.1111/j.1432-1033.2004.04258.x
present in cell extracts of succinate/glucose-grown cells;
therefore, the s ynthesis ofthe e nzyme was indu cible. Table 1
shows a summary of a typical enzyme purification. The
enzyme was purified...
... chame-
leons, in which small alterations in the environment can
cause drastic changes in the reactivity ofthe active species
[76]. Further support in favour of th e idea ofthe involve-
ment of a high-valent ... intermediate [11]. The sum of
these findings points at the involvement of more than one
active oxidant in the diverse types of P450-catalyzed
substrate processing [12–15].
The g oal of t he p resent ... vis-a
`
-vis the TSR the ory . On the
other h and, intractability of h igh-energy intermediates such
as ferryl precludes facile experimental proof ofthe Ôtwo-
stateÕ proposal owing to l ack of a ppropriate...
... productive binding of steroid substrates:
in the first, a (small) sub population of enzyme binds
directly the steroid in the reactive conformation, with
the remainder ofthe enzyme binding the unreactive
conformation. ... grossly perturb the
overall framework ofthe enzyme or the active site. The active sites
of the enzymes are shown in stick format with the sigmaA weigh-
ted 2F
0
-F
c
density for the Y186F mutant ... bond in
close proximity ofthe N5 atom. A putative flipping
motion ofthe progesterone molecule along an axis
parallel to the FMN plane aligns the C1-C2 with the
plane ofthe isoalloxazine ring...
... An
additional source of superoxide anion radicals is com-
plex I [14]. The degree of production of ROS by the
respiratory chain depends on the type of tissue, the
kind of substrates driving oxidative phosphorylation
(either ... pmolÆ
s
)1
Æmg
)1
of mitochondrial protein. The decrease in the
fluorescence signal recorded at the moment of reoxy-
genation is caused by dilution due to the addition of
1 mL medium.
To investigate whether the ... permeabilization of the
membrane system. Even in the additional presence
of 10 mm TEMPOL, significant permeabilization of
the mitochondrial membrane occurred, which was
shown by the NADH sensitivity of state...
... oxygen affects the formation of the
quinonemethide. Then, water attacks the Ca position in
the quinonemethide and the b-aryl ether linkage is cleaved.
Another water molecule then attacks the Ca position ... Analysis ofthe products ofthe reaction
revealed the presence of guaiacylglycerol (GG) and 4MU
(data not shown).
Localization of enzymatic activity
To confirm the localization ofthe b-aryl ether ... 6).
To clarify the substrate specificity ofthe b-aryl ether
cleavage enzyme, we synthesized the substrates GOUbz
Fig. 7. Substrate specify ofthe b-aryl ether
cleavage activity of 2BW-1. Structure...
... either distorted or the access of a
cation to the site is blocked by the p resence of a side chain
of a b asic residue. I n case ofthe IIAB s ite (Fig. 2), the
acidic re sidue a cting a s the ... sites in the first domain.
The observed conformation ofthe other domains does not
allow binding of divalent c ations. The molecular mechanism
of calcium binding of plant annexins requires further ... fold even among
the members ofthe plant subfamily of annexin proteins. The
fold is comprised ofthe arrangement o f four a-helical
domains into two modules, which are held together by polar
interactions....
... formed
between the carbonyl group ofthe ketose substrate
(f6p, s7p) and the e-amino group of a lysine residue of
the active site ofthe enzyme, leading to the formation
of either g3p or e4p ... rerouting of metabolic
fluxes was observed. The incorporation of genetic
knowledge into the metabolic network model for the
nonoxidative branch ofthe PPP introduced the possi-
bility of modeling the ... expected, the
largest differences are found for the PPP split-ratio and
the fluxes ofthe nonoxidative branch ofthe PPP.
The minimized covariance-weighted sum of
squared residuals (SS
res
) in these...
... consist in this. When they
need, in the course of their business, the use ofthe floating capital which
they have invested in the bank, they can borrow ofthe bank to the amount
of their stock, and ... specie as they
had, or sent it out ofthe kingdom.
The Royal Bank and the Company ofthe Indias were now incorpo-
The History of Banks/9
The conveniency of these notes soon spread them over the kingdom;
and ... gap in the circulation, the banks,
having constantly the terror of a run before their eyes, are obliged to
enlarge it. By the diminution of its total amount, the value ofthe parts of
which the...
... functions of
b-arrestins, or by modulating the activity of protein
kinase C, which can activate Raf isoforms at the apex of
the ERK1/2 module (reviewed in [42]).
Redox regulation at each of these ... especially those in the MKP family,
share a docking domain with high affinity for binding
ERK1/2 [58]. Several of these phosphatases are restricted
either to the cytoplasm (e.g. MKP3) or to the nucleus ... indicate
the presence of phosphorylated ERK1/2 in association with
fibrillar bundles, distended mitochondria and autophago-
somes [52]. These discrete accumulations of ERK1/2 in the
cytoplasm raise the...
... parts: the topic and the
controlling idea.
- The topic sentence is the subject ofthe paragraph.
Eg:
The color yellow is the color of mental activity.
The topic: the color yellow
- The controlling ... structure, the sub-title, and the discourse ofthe news
story.
From which, I would like to draw some difficulties of Vietnamese
readers when they translate the news story.
To sum up, the scope ofthe ... study which introduces my reasons and
aims to write the thesis. This part includes the aims, scopes, methods and
design ofthe study.
Part B is the main part ofthe study that consists of three...
... wake at 6 a.m., but nap for two hours or so in the early afternoon. Thus the influence on one's
sleep pattern is worthy of consideration when choosing an occupation.
...