virulence mechanisms and persistence strategies of the human gastric pathogen helicobacter pylori
Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx
Ngày tải lên :
15/02/2014, 01:20
... II The hood domain is mainly built up by residues from the C-terminus and consists of a two-stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix from the C-terminus (Fig 1A) The ... Lys173 interacts with both O6 and N7 (Fig 1C) The 2¢OH of the ribose is hydrogen bonded to the main chain and the side chain of Asp142 (Fig 1C) Both hydroxyl groups of the ribose are involved in ... Asp142, 2¢ and 3¢OH of the ribose and three water molecules with ˚ coordination distances of approximately 2.4 A The phosphate of the GMP is hydrogen bonded to main chain and side chain atoms of amino...
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Báo cáo khoa học: Effects of cardiomyopathic mutations on the biochemical and biophysical properties of the human a-tropomyosin docx
Ngày tải lên :
16/03/2014, 18:20
... for the wild type (WT) and mutant Tms The uncertainties listed are the standard errors of the mean and included the uncertainty in the determination of protein concentrations The values are the ... probe Thus, the analysis of the melting profile of human Tm was enhanced by the use of different probes The mutations affect the stability of the protein Heller et al ([19] and references therein) ... parameters for the thermal-induced unfolding of wild type (WT) and mutant Tms The values are the mean ± standard deviation of at least three experiments Tm1 is the midpoint of the thermal transition...
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Báo cáo khoa học: Molecular cloning and functional expression of the human sodium channel b1B subunit, a novel splicing variant of the b1 subunit potx
Ngày tải lên :
16/03/2014, 23:20
... significantly in the presence or absence of the b1B subunit, whereas, under the same conditions, the rate of inactivation was increased in the presence of the b1 subunit The effects of b1B were further assessed ... Functional expression of the human b1B subunit with NaV1.2 in Xenopus oocytes To explore the regulatory function of the human b1B subunit, we injected cRNA of human b1B, as well as cRNA of the sodium channel ... part of intron adjacent to exon (Fig 1C and Table 1) As the site of divergence between the b1 and b1B subunit cDNAs was located precisely at the exon 3/intron boundary of the SCN1B gene, the human...
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Báo cáo khoa học: The C-terminal region of CHD3/ZFH interacts with the CIDD region of the Ets transcription factor ERM and represses transcription of the human presenilin 1 gene pot
Ngày tải lên :
30/03/2014, 09:20
... implicated in the development of AD because of its role in cleavage of the amyloid precursor protein (APP) and the production of Ab peptide, which is central to the pathogenesis of AD [9] Similarly, ... indicating the crucial importance of these two Ets motifs [15] In addition to controlling the level of gene expression, Ets factors may direct the choice of the promoter elements in play, and therefore, ... acids, the Ets domain, the CIDD and the C-terminal domain The fragment of cDNA included in the bait used for two-hybrid screening of the brain cDNA library included amino acids 96–510 at the C-terminus...
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Báo cáo khoa học: Expression and characterization of soluble forms of the extracellular domains of the b, c and e subunits of the human muscle acetylcholine receptor pot
Ngày tải lên :
30/03/2014, 10:20
... contains the major part of the binding site for the cholinergic ligands The two sites are nonequivalent, one being formed at the interface between one a subunit and the c ⁄ e subunits and the other ... report, we present the expression and characterization of the ECDs of the b, c and e subunits of the human muscle AChR We describe their expression in a soluble, glycosylated form and in satisfactory ... Purification and deglycosylation of the AChR ECDs (A) Schematic representation of the constructs used for expression of the b1–221, c1–218 and e1–219 ECDs of the human AChR in yeast P pastoris The arrowhead...
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Báo cáo khoa học: Characterization of the 5¢ untranslated region of a and b isoforms of the human thromboxane A2 receptor (TP) Differential promoter utilization by the TP isoforms doc
Ngày tải lên :
31/03/2014, 09:20
... the current study, we sought to investigate the molecular basis of the differential expression of TPa and TPb isoforms Our initial aim was to map the patterns of exon usage in the 5¢ UTR of the ... number of the diverse superfamily of GPCRs, not least among the prostanoid receptor subfamily of GPCRs [53] The EP3 subtype of the PGE2 receptors is one of the most heavily spliced among all of the ... Ó FEBS 2002 Analysis of the TPa and TPb mRNAs (Eur J Biochem 269) 4059 investigating the expression of the mRNAs encoding the TPs throughout a range of cell and tissues of particular relevance...
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báo cáo hóa học:" Biomechanical and system analysis of the human femoral bone: correlation and anatomical approach" docx
Ngày tải lên :
20/06/2014, 00:20
... of division of the meaning of each linear parameter on the meaning of the transverse diameter of the femoral diaphysis) In this case, the deviation of the measurement becomes unimportant Furthermore, ... in the 1st and 3rd classes; in the 4th class, the parameter is related to the vertical diameter of the head and the transverse size of the proximal epiphysis The fourth group characterizes the ... Transverse size of the proximal epiphysis - intertrochanteric distance - Diaphysis-neck angle - Anteversio of the neck - Rotation of the head - The length of the lateral condyle - The length of the medial...
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Báo cáo khoa học: The stop transfer sequence of the human UDPglucuronosyltransferase 1A determines localization to the endoplasmic reticulum by both static retention and retrieval mechanisms docx
Ngày tải lên :
07/03/2014, 16:20
... membrane expression [6] In the absence of positive transport signals, the localization of a protein in the ER may result from the properties of the TMD and its interaction with the membranes It has ... disruption of the dilysine motif KSKTH of UGT1A by mutation of lysine to serine residues or by extending the length of the cytoplasmic tail to relocate the dilysine from the critical positions )3 and ... from the ER into the latter compartment in the secretory pathway A similar behaviour was observed in the case of CD4– TMD ⁄ CTmyc (data not shown) Immunofluorescence A Fig The TMD and CT of the...
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Báo cáo y học: "Mechanical complications and reconstruction strategies at the site of hip spacer implantation"
Ngày tải lên :
26/10/2012, 09:53
... 8) At the time of prosthesis reimplantation, the spacer head can be easily removed and the modular prosthesis parts (neck and head) placed This procedure offers a stable fracture treatment and ... removal, and insertion of a cement-coated modular prosthesis with a spacer head The cement mantle of the prosthesis is also antibiotic-loaded according to the sensitivity profile of the causative organism ... lag screws and Charnley prostheses, respectively [5] The authors reported that all constructs based upon the Charnley prostheses and the commercial spacers did not fail at 3000 N; the other two...
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Contrastive analysis on english and vietnames proverbs referring to parts of the human body
Ngày tải lên :
12/12/2013, 00:03
... body The position of proverbs referring to part of the human body Parts of the human body 21 21 21 The classification of parts of the human body according to position Graduation thesis ... understand the equality, sympathy of the husband and wise the function of education is to contribute to directing the peoples feelings towards the good orientation in the relationship of the husband ... diversified (multiple form) The classification of parts of the human body according to the position In the system of the proverbs, the system of words referring to parts of the human body is divided...
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The transference of meaning through class of words denoting parts of the human body in english and vietnames
Ngày tải lên :
18/12/2013, 21:45
... earth The hand of a child - The hand of a lock The heart of a man - The heart of a matter 37 The tongue of a man - The tongue of a shoe The tooth of a person - The tooth of a comb The head of a ... hands of a farm 13 The heel of a person - The heel of the hand 14 One's leg - The leg of a trip 15 One's brain - The brains of a family 16 The bosom of a person - The bosom of the church 17 The ... The head of the page The scar left on one's kin - The scars of war The arm of a person - The arm of jacket 10 One's breast - The chest 11 One's foot - The foot of sock 12 One's hands - The hands...
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Tài liệu Báo cáo khoa học: Roles of the human Rad51 L1 and L2 loops in DNA binding doc
Ngày tải lên :
19/02/2014, 06:20
... in these eukaryotic and archaeal proteins (Fig 1A) Like the case of bacterial RecA, the L1 and L2 loops of the eukaryotic Rad51 proteins face A Roles of the HsRad51 L1 and L2 loops inside of their ... Results Strategy of mutational analysis In order to study the functions of the L1 and L2 loops of HsRad51, we examined the effect of replacing the aromatic residues in the L1 and L2 loops with ... monomer of poly(dT), showing that the changes were mainly due to the binding of the first DNA, while the binding of the second DNA had less influence (Fig 5A) To ensure that the estimation of the protein:poly(dT)...
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Tài liệu Báo cáo khoa học: Topology, tinkering and evolution of the human transcription factor network doc
Ngày tải lên :
19/02/2014, 07:20
... compares the studied network with randomized versions of it with the same size and degree distribution The so-called Z-score quantifies the difference between the studied network and an ensemble of randomized ... functions, and topological features retain functionality and phylogeny However, the nature of the connections between these factors needs to be understood at the level of the protein domain The global ... from any other If dmin (Pi, Pj) is the length of the shortest path connecting proteins Pi and Pj, then L is defined as: Compared with pure random ER and SF networks, biomolecular webs show the characteristic...
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Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx
Ngày tải lên :
19/02/2014, 16:20
... proliferation of stelleate cells and synthesis of collagen [36,37], the in vitro data presented here may be relevant for understand some of the complex mechanisms responsible for the development of fibrosis ... cells and molecules of the immunological system in hepatic injury and repair Understanding the interdependence between the metabolism of iron and lipids [14,56,57] may be relevant in a variety of ... loading of the liver epithelial cell line HepG2 influenced the expression of immune regulatory molecules known to function as ligands of selected subsets of T cells, such as MHC class I and II,...
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Tài liệu Báo cáo khoa học: Characterization of promoter 3 of the human thromboxane A2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity docx
Ngày tải lên :
19/02/2014, 16:20
... and D; lane 1) and HEK293 (C and D; lane 2) cells The positions of the molecular size markers (kDa) are indicated to the left and right of the (C) and (D), respectively, whilst the position of ... regions of Prm3 The following lists the identities of the Prm3 gene fragments and corresponding plasmids generated in either pGL3Basic (pGL3b) or pGL3Enhancer (pGL3e) vectors and the identity of the ... Characterization of the 5¢ untranslated region of alpha and beta isoforms of the human thromboxane A2 receptor (TP) Differential promoter utilization by the TP isoforms Eur J Biochem 269, 4058–4073 26 Quandt...
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Tài liệu Báo cáo khoa học: Different mechanisms for cellular internalization of the HIV-1 Tat-derived cell penetrating peptide and recombinant proteins fused to Tat docx
Ngày tải lên :
21/02/2014, 03:20
... amounts of the rhodamine-labeled Tat peptide, as indicated in the ®gure Comparative FACS analysis of the internalization of the full-length Tat protein construct and the Tat CPP Differences in the mechanisms ... abolished the internalization of the GFP-fused Tat protein but did not alter the uptake of the Tat CPP These biochemical evidences con®rmed a pathway for the entry of the Tat peptide unrelated to the ... cells The internalization of the peptide in these cell lines was monitored in parallel by ¯uorescence microscopy and by FACS scan analysis The ®rst technique con®rmed the uptake of the peptide and...
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Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx
Ngày tải lên :
21/02/2014, 15:20
... Model structure of human LPL All model structures of dimeric human LPL were constructed using the INSIGHT II program and were based on the crystal structures of the human, porcine, and horse PLs ... illustrates the overall saddle shape of the dimeric structure and the key heparin-binding residues in the N-terminal domain held coordinately by the two subunits on the top of the hollow (Fig 1A) The ... the C-terminal domain of apoCII interacts with the interface of the N- and C-terminal domains of LPL and part of the lid surface [32] Because the functional importance of LPL’s C-terminal domain...
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Báo cáo khoa học: Adenine nucleotides inhibit proliferation of the human lung adenocarcinoma cell line LXF-289 by activation of nuclear factor jB1 and mitogen-activated protein kinase pathways doc
Ngày tải lên :
07/03/2014, 12:20
... factors, and cytokines Further detailed investigations are needed to unravel the subtype of P2Y receptor mediating the observed response and the functionality of the proteins in the signaling mechanisms ... advances indicate a number of links between the activation of p38 kinase and the DNA checkpoint pathways and their possible interaction in the modulation of cell cycle control and DNA mismatch repair ... translocation of ERK1 ⁄ and of p50 NF-jB1 to the nucleus suggests a role of both proteins in the regulation of cell cycle progression by extracellular nucleotides Sustained activation of the ERK1 ⁄ and...
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Báo cáo khoa học: Purification and structural study of the b form of human cAMP-dependent protein kinase inhibitor pdf
Ngày tải lên :
07/03/2014, 15:20
... studies of the catalytic subunit of the cAMP-dependent protein kinase Its ability to readily alter the PKI coding sequence could permit further studying of human PKIb and understanding of the function ... component bands, determined by band fitting of the absorbance spectrum of Fig 4A, are shown in Fig and Table From Table 2, the sum of individual amide I¢ intensity and the intensity percent of each ... indicated by the presence of a very strong negative band at 198 nm The negative band near 220 nm results from overlapping of the bands of b-sheet (215 nm) and a-helix Table Fractional composition of secondary...
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Báo cáo khoa học: Solution structure and backbone dynamics of the XPC-binding domain of the human DNA repair protein hHR23B docx
Ngày tải lên :
07/03/2014, 17:20
... further our knowledge with respect to the mechanisms of action of these proteins, it is crucial that we define the precise boundaries of the STI1-homologous domain and compare the structures of these ... [33] The errors of R1 and R2 were estimated from the errors in the single exponential decay fitting, and those of the 15N-1H NOEs were obtained from the difference of two independent experiments The ... values of error were adjusted for the proper tensor2 analysis, by increasing of the errors from the fitting (1.75 and 2.0 times for the relaxation data of 500 and 600 MHz, respectively), in which the...
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