viral replication strategies manipulation of er stress response pathways and promotion of ires
Báo cáo y học: "Control of viral replication after cessation of HAART" pdf
Ngày tải lên :
10/08/2014, 05:22
... ITMA, Antwerp, Belgium 4Department of Medicinal Chemistry, University of Antwerp (UA); Faculty of Medicine and Pharmacy, Free University of Brussels (VUB) and Department of Gynecology and Obstetrics, ... Catholic University of Leuven (KUL), Belgium 5Department of Biomedical Sciences, Faculty of Pharmaceutical, Veterinary and Biomedical Sciences, University of Antwerp and Faculty of Medicine and Pharmacy ... the reference strains and viruses of rebounders since the amount of p24 produced in PHA stimulated PBMC remains much lower over time in comparison to viruses of rebounders and the reference strain...
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Báo cáo y học: " Transient expression of bC1 protein differentially regulates host genes related to stress response, chloroplast and mitochondrial functions" pptx
Ngày tải lên :
11/08/2014, 21:21
... (DEGs) were amplified by different anchored and arbitrary primer pairs (Table 1; Figures 1A-F) The anchored and arbitrary are random decamer primers, and used as reverse and forward primer for ... strand cDNA, μl of 10× PCR buffer, μl MgCl (25 mM), μl of dNTPs (10 mM each), μl of anchord primer (250 ng/μl), μl of arbitrary primer (100 ng/μl), 0.5 μl of Taq DNA Polymerase (5 U/μl; Fermentas, ... the response of bC1 of ChLCB under 35S cauliflower promoter are related to the chloroplast and mitochondria and are involved in the ATP synthesis, act as electron carriers for respiration and...
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Báo cáo y học: "Stress, the stress response system, and fibromyalgia" ppt
Ngày tải lên :
09/08/2014, 10:20
... particularly after trauma, a sympathetic– afferent interaction can be established both at the peripheral and central levels In a rabbit model, after peripheral nerve injury, sympathetic stimulation and norepinephrine ... of (page number not for citation purposes) Chrousos GP, Gold PW: The concepts of stress and stress system disorders Overview of physical and behavioral homeostasis JAMA 1992, 267:1244-1252 Hoffman ... part of a series on Biology and therapy of fibromyalgia edited by Leslie Crofford 16 Other articles in this series can be found at http://arthritis-research.com/articles/ review-series.asp?series=ar_fibromyalgia...
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Báo cáo y học: "Is fibromyalgia a cardiovascular disease? A comment on Martinez-Lavin’s review ‘Stress, the stress response system, and fibromyalgia’" ppsx
Ngày tải lên :
09/08/2014, 10:21
... Martinez-Lavin M: Biology and therapy of fibromyalgia: Stress, the stress response system, and fibromyalgia Arthritis Res Ther 2007, 9:216 Fontenele JB, Félix FHC: Fibromyalgia and related medically ... Therapy Vol No Félix and Fontenele Competing interests The authors cite their own work on the subject that is currently in press There are no potential sources of conflicts of interest References ... pain Metabol Clin Exper 2007, 56:8793 Chaiamnuay S, Bertoli AM, Fernández M, Apte M, Vilá LM, Reveille JD, Alarcón GS: The impact of increased body mass index on systemic lupus erythematosus Data...
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MYCOBACTERIAL DORMANCY AND PERSISTENCE MOLECULAR MECHANISMS CONTROLLING STRESS RESPONSE, SURVIVAL AND ADAPTATION
Ngày tải lên :
22/09/2015, 15:19
... time-course studies of bacterial stress responses wherein large differences between protein and mRNA abundance changes were detected [205, 206] Clearly, our understanding of perturbed systems is ... (containing both persisters and non-persisters), with physiological stresses in the environment pushing a greater part of the population to become dormant persisters [186] These dormant bacteria are thought ... antibiotic tolerance of dormant mycobacteria Further characterization of the close links between lipid catabolism and antibiotic resistance is presented in Chapter of this thesis, wherein we highlight...
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Tài liệu Báo cáo khoa học: Analyzing changes of chromatin-bound replication proteins occurring in response to and after release from a hypoxic block of replicon initiation in T24 cells pptx
Ngày tải lên :
21/02/2014, 00:20
... start of an experiment Thereby, Cells were washed once with NaCl/Pi and twice with hypotonic buffer (20 mM Hepes, pH 7.5, 20 mM NaCl, mM MgCl2) and suspended in 10 mL of hypotonic buffer After 10 ... after medium renewal; (B) cells after medium renewal and h of hypoxia (200 p.p.m.); (C) the same cells after h of hypoxia (200 p.p.m) and h aerated incubation Fig Alkaline sedimentation patterns ... prereplication complex as well as Cdk2, which is considered to be (one of) the activating kinase(s) of the complex, after medium renewal before and at the end of hypoxic gassing as well as under...
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Báo cáo khoa học: Yeast oxidative stress response Influences of cytosolic thioredoxin peroxidase I and of the mitochondrial functional state pot
Ngày tải lên :
16/03/2014, 14:20
... functions of cTPxI as peroxidase and as molecular chaperone, suggested by mixtures of low and high molecular weight oligomeric structures observed in cells challenged with H2O2 and (b) the capacity of ... development of different kinds of cancer [56–60] and neurodegenerative diseases [61,62] Experimental procedures Yeast strains and growth conditions The following S cerevisiae strains were used in ... indicators of oxidative stress [41–43] To determine whether TSA1 deletion and the mitochondrial dysfunction can generate stressful conditions, the levels of sulfhydryl groups in the reduced state were...
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Báo cáo khoa học: The crystal structure of annexin Gh1 from Gossypium hirsutum reveals an unusual S3 cluster Implications for cellulose synthase complex formation and oxidative stress response potx
Ngày tải lên :
17/03/2014, 03:20
... state of monomeric CesA protein, oxidation leads to homo- or heterodimerization of CesA by formation of intermolecular disulfide bonds (involving the Cys residues of the zinc finger motif) and release ... oxidative stress response by annexins Materials and methods Purification of recombinant protein Cloning and construction of an N-terminal His4-fusion protein has been described earlier [20] The ... bell pepper [19] A detailed elucidation of the oligomerization behaviour of plant annexins yielded calcium-independent monomer-trimer equilibria for annexins 23(Ca38), 24(Ca32), and Gh1, whereas...
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Báo cáo khoa học: Identification of sodium salicylate as an hsp inducer using a simple screening system for stress response modulators in mammalian cells pptx
Ngày tải lên :
17/03/2014, 10:20
... cells and a concomitant increase of thermoresistance of cells Thus, SA may be used for the protection of cells against deleterious stressors Furthermore, several neurodegenerative disorders including ... trimeric and hyper-phosphorylated active form from inactive monomers in response to various forms of stress and induces the transcription of hsp genes [8] SA is shown to trigger HSF differently than ... the acquisition of resistance of cells against various forms of stress [1] Here we showed that SA induced the activation of HSF, the transcription of hsp genes and the accumulation of Hsps in various...
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Báo cáo khoa học: The yeast stress response Role of the Yap family of b-ZIP transcription factors The PABMB Lecture delivered on 30 June 2004 at the 29th FEBS Congress in Warsaw potx
Ngày tải lên :
30/03/2014, 16:20
... environmental stress response (ESR), largely mediated by the two transcription factors Msn2 and Msn4 [7] Here, we review the response of the budding yeast S cerevisiae to several different forms of stress ... proteins and the yeast response to stress expression programmes common to all environmental stress responses are at play [1] Specific forms of stress such as heat shock and some forms of oxidative stress ... overexpression confers resistance to a plethora of stress agents such as cadmium, cerulenin and 1,10-phenanthroline among others, suggesting a role in the response to drug stress Indeed, several...
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Báo cáo sinh học: "Molecular and cellular correlates of the CIITA-mediated inhibition of HTLV-2 Tax-2 transactivator function resulting in loss of viral replication" pot
Ngày tải lên :
18/06/2014, 22:20
... mediated inhibition of Tax-2 We found that both CIITA and NF-Y interact in vivo with Tax-2 We identified both an N-terminal and a Cterminal region of CIITA interacting with the viral transactivator, ... gift of Prof Bertazzoni, University of Verona, Italy NF-YB cDNA (pcDNA3mycNF-YB) has been described [33] Transient transfections, Co-Immunoprecipitation and Western blotting Human embryo-derived ... 1-252 and fCIITA 253-1130 fragments These results indicate a complex pattern of interaction between CIITA and Tax-2 with at least two regions of CIITA, encompassing the N-term1-252 and at the Cterm...
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Báo cáo hóa học: " Modulation of viral replication in macrophages persistently infected with the DA strain of Theiler''''s murine encephalomyelitis virus" docx
Ngày tải lên :
20/06/2014, 01:20
... with the ED of each compound Culture supernatants and cells were harvested at the start of the experiments and each following 24 h during days, where after the infectivity was determined by plaque ... Samples, consisting of supernatants and cells, were analyzed after three rounds of freezing and thawing L929 cells, originally derived from normal subcutaneous areolar and adipose tissue of a 100-day-old ... Theiler's virus persistence in the central nervous system of mice is associated with continuous viral replication and a difference in outcome of infection of infiltrating macrophages versus oligodendrocytes...
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o cáo hóa học:" Molecular and cellular correlates of the CIITA-mediated inhibition of HTLV-2 Tax-2 transactivator function resulting in loss of viral replication" potx
Ngày tải lên :
20/06/2014, 04:20
... mediated inhibition of Tax-2 We found that both CIITA and NF-Y interact in vivo with Tax-2 We identified both an N-terminal and a Cterminal region of CIITA interacting with the viral transactivator, ... gift of Prof Bertazzoni, University of Verona, Italy NF-YB cDNA (pcDNA3mycNF-YB) has been described [33] Transient transfections, Co-Immunoprecipitation and Western blotting Human embryo-derived ... 1-252 and fCIITA 253-1130 fragments These results indicate a complex pattern of interaction between CIITA and Tax-2 with at least two regions of CIITA, encompassing the N-term1-252 and at the Cterm...
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Báo cáo y học: " Stress response regulators identified through genome-wide transcriptome analysis of the (p) ppGpp-dependent response in Rhizobium " docx
Ngày tải lên :
09/08/2014, 22:23
... proper assembly of unfolded proteins and refolding of misfolded proteins generated under stress conditions [74] DnaK is also involved in chromosomal DNA replication and is part of the osmotic stress ... long-term survival, pellets of overnight cultures of wild-type and mutant strains were washed and resuspended in 10 mM MgSO at an OD 600 of 0.5 A volume of 100 ml of AMS medium (10 mM NH4Cl and ... suggests that the function of (p)ppGpp differs during active growth and growth arrest, possibly through involvement of other regulators To further understand the impact and role of the alarmone during...
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Báo cáo y học: " Management of HIV-1 associated hepatitis in patients with acquired immunodeficiency syndrome: role of a successful control of viral replication" ppsx
Ngày tải lên :
10/08/2014, 05:22
... mm.) and an increase of LFTs (ALT 119 UI/l and AST 270 UI/l) No diabetes or other metabolic disorders were present Antiretrovirals were discontinued and the patient underwent to laboratory and ... observed and after one month of therapy he showed an immune-virological recovery with a persistently improvement of LFTs (Figure 1) Conclusions Involvement of the liver during the course of HIV-1 ... use of specific antiretroviral drugs (hyperbilirubinemia and gamma-glutamil-transpeptidase mild increases with indinavir and nevirapine, respectively) HBV and HCV serology were negative, whereas...
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báo cáo khoa học: " The NAC domain-containing protein, GmNAC6, is a downstream component of the ER stress- and osmotic stress-induced NRP-mediated cell-death signaling pathway" pptx
Ngày tải lên :
11/08/2014, 11:21
... position GmNAC6 downstream of the NRPs in the ER stress- and osmotic stress- integrating Figure The osmotic -stress and ER- stress signal-integrating pathway ER stress and osmotic stress activate two independent ... two stress signals promoted a synergistic accumulation of GmNAC6 transcripts The synergistic induction of gene expression by the combination of ER stress and osmotic stress inducers is one of ... combination of both stress signals intensifies the output of the different pathways upon NRP expression; therefore, NRPs serve as molecular links that integrate the ER stress and osmotic stress responses...
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báo cáo khoa học: " Characterization of singlet oxygen-accumulating mutants isolated in a screen for altered oxidative stress response in Chlamydomonas reinhardtii" ppsx
Ngày tải lên :
11/08/2014, 11:21
... expression of stress response genes like GPXH might explain the HL resistance of these mutants and shows the important role of GPXH and other defense genes in the photooxidative stress response of photosynthetic ... Cells were grown heterotrophically to a density of × 106 cells ml-1, and 20 ml were aliquoted into a sterile Pyrex® petri dish (14 cm diameter) and exposed to 30 - 60 mJ cm-1 of UV light Cells were ... BBF designed and performed the experiments, evaluated the data and drafted the manuscript RILE and KKN contributed to conception and design of the experiments, data interpretation and drafting...
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Báo cáo y học: " Effective inhibition of HCMV UL49 gene expression and viral replication by oligonucleotide external guide sequences and RNase P" pptx
Ngày tải lên :
12/08/2014, 04:20
... expression of IE2, UL44, pp28, and gB (Table 1) The observed level of inhibition of UL49 expression was consistent with the extent of the reduction of viral growth HCMV is a member of the human herpesvirus ... product bound versus EGS concentration The values were the average of three experiments EGS internalization and viral infection Lipofectamine 2000 reagent (GIBCO) was diluted in 100 μl of Opti-MEM ... http://www.virologyj.com/content/7/1/100 of an immediate-early gene (IE) and an early-late gene (UL44) Moreover, Western analyses were performed to determine the levels of viral protein IE2, a viral immediate-early...
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báo cáo khoa học: " Sequencing analysis of 20,000 full-length cDNA clones from cassava reveals lineage specific expansions in gene families related to stress response" docx
Ngày tải lên :
12/08/2014, 05:20
... profile of the assembly of cassava ESTs Cluster Cluster profile of the assembly of cassava ESTs The graph presents the number of clones per assembled scaffold; it should be noticed that over ... performed using the WEGO web server [103], terms overrepresented in one of the two groups (p-value < 0.05 Pearson Chi-Square test and more than genes per category) were considered for further ... and other structural features Given the above, the importance of our effort is not only measured in terms of the amount of sequences captured, but also in terms of the quality and relevance of...
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Báo cáo y học: " Some ABCA3 mutations elevate ER stress and initiate apoptosis of lung epithelial cells" pptx
Ngày tải lên :
12/08/2014, 13:22
... in the ER, caused significant increase of the ER stress and early and late apoptosis markers in the A549 cells (Figure 4, and 6) The ER stress caused by R280C mutation was slightly lower and surface ... more prone to further elevation of ER stress upon exposure to an external stressor L101P and to a lesser extent R280C mutation induce apoptosis of A549 cells Since prolonged ER stress can activate ... retention of the L101P mutant in the ER and ability of WT, R43L and R280C to progress further from the ER to the Golgi C) Functional assay Trafficking/folding defect and ER accumulation of L101P...
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