validation and analysis of microarray data
Báo cáo y học: " Interactions between Nef and AIP1 proliferate multivesicular bodies and facilitate egress of HIV-1" potx
... production of viral partcles and intracellular levels of Nef Virus and Gag VLP production, virion and Gag VLP isolation and Gag expression To assess effects of Nef during the production of new viral ... binding and eliminated effects of Nef on the proliferation of MVBs and release of viral particles We conclude that by connecting GagPol and AIP1, Nef acts as a chaperone the production and optimal ... removal of CD4 from HIV-1 Env and emphasizes the importance of Nef during late stages of the viral replicative cycle in these cells Nef can substitute for the function of the L domain of Gag The...
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in vitro transcription and translation protocols
... vol of each individual 10 mM stock of ATP, UTP, and CTP, and vol of water), PL of GTP (0.5 mM, dilute 10 rnM stock 1:20 with water), p.L of the cap analog m7G(5’)ppp(5’)G (5 mM), and pL of DNA ... 0.6 p,L of 10 mCi/mL a-[32P]dATP, PL of 15 mM PNAD, 0.5 pL of 10 mg/mL BSA, PL of U/pL E cull DNA polymerase I, 0.5 PL of U/p,L RNaseH, 0.25 l.tL of U@L E coli DNA ligase, and 56.4 l,tL of H20 ... strand: 10 p.L of annealed DNA, p.L of 10X Klenow buffer, 0.5 pL of mM dNTPs, 10 U of Klenow fragment, and Hz0 up to a final volume of 50 pL Incubate at 37OCfor h 3.6.2 E coli Transforming and...
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Báo cáo sinh học: " Stimulation of poliovirus RNA synthesis and virus maturation in a HeLa cell-free in vitro translation-RNA replication system by viral protein 3CDpro" docx
... effect on the yield of either of the kinds of RNA products (Fig 3B and 3C, compare lanes and 3) In the presence of both 3Cpro(C147G) and 3CDpro(3CproH40A), however, the synthesis of both products ... protein (data not shown) A Western blot analysis of the particles in the pellets indicated the presence of VP0 but no VP2 (data not shown) 10 3CDpro produced in of the amount of VP0 and extra ... the polyprotein, formation of membranous replication complexes, uridylylation of the terminal protein VPg, synthesis of minus and plus strand RNA, and encapsidation of the progeny RNA genomes...
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Báo cáo khoa học: "Stochastic Language Generation Using WIDL-expressions and its Application in Machine Translation and Summarization" pot
... consists of all the possible interleavings of strings from , , and the proba The bility values are induced by and distribution function is dened either explicitly, over (the set of all permutations of ... Figure and a nal WIDL-graphs have an initial vertex vertex Vertices , , and with in-going edges labeled , , and , respectively, and vertices , , and with out-going edges labeled , , and , respectively, ... labeled between two states and in exists if there exists a vertex in the description of and a vertex in the description of such that there exists a path in between and , and is the only -labeled...
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Báo cáo khoa học: In vitro selection and characterization of a stable subdomain of phosphoribosylanthranilate isomerase potx
... 000 of 87 of 68 of 87 11 of 87 38 of 87 < 1.1 < 1.5 2.3 13 44 arginine of the new epitope, and removed the fragment of the PRAI (ba)8-barrel corresponding to a6(ba)7)8 The remaining fragment of ... deletion of one quarter of the strands that make up the b-barrel core of the protein, CD and fluorescence data suggest that trPRAI retains the same degree of a ⁄ b structure as PRAI and that it ... denaturation of PRAI and trPRAI-His as monitored by CD at 219 nm Raw data and smoothed curves are shown (B) Fluorescence emission spectra of PRAI and trPRAI-His The excitation wavelength was 280 nm and...
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Báo cáo hóa học: " In Vitro Structural and Functional Evaluation of Gold Nanoparticles Conjugated Antibiotics" doc
... property of sodium borohydride and antibiotics were used to reduce H[AuCl4] The seeding of Gnps was done in presence of the antibiotics (Ampicillin, Streptomycin and Kanamycin, Fig 1) individually and ... number distribution of the hydrodynamic diameter of bare Gnps and Gnps conjugated antibiotics (b) represents the intensity distribution of the hydrodynamic diameter of the free Gnps and the antibiotics ... bluish because of the presence of some larger particles too As DLS study showed size distribution of Gnps conjugated particles, we then wanted to visualize and validate the size of the particles...
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Báo cáo hóa học: " In Vitro Structural and Functional Evaluation of Gold Nanoparticles Conjugated Antibiotics" pdf
... property of sodium borohydride and antibiotics were used to reduce H[AuCl4] The seeding of Gnps was done in presence of the antibiotics (Ampicillin, Streptomycin and Kanamycin, Fig 1) individually and ... number distribution of the hydrodynamic diameter of bare Gnps and Gnps conjugated antibiotics (b) represents the intensity distribution of the hydrodynamic diameter of the free Gnps and the antibiotics ... bluish because of the presence of some larger particles too As DLS study showed size distribution of Gnps conjugated particles, we then wanted to visualize and validate the size of the particles...
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Báo cáo khoa học " In vitro cytostatic and immunomodulatory properties of the medicinal mushroom Lentinula edodes " pdf
... the prevention and treatment of many diseases for thousands of years especially in the Orient (Israilides and Philippoussis, 2003; Kidd, 2000; Wasser and Weis, 1999) A plethora of medicinal effects ... the means of SI in repeated testing of the extracts were evaluated by analysis of variance (ANOVA), and calculations were done by the EP16 programme Results and discussion Chemical and FTIR analyses ... The bands a at 2850–3000 cmÀ1 (nCH2 and nCH3) and b at 1745 cmÀ1 and 1702 cmÀ1 (nCO), related to vibrations of alkyl chain, ester and free carboxyl groups, respectively, are indicative of lipids,...
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Báo cáo khoa học: "In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood" ppt
... tnecseroulf gniwollof ehT SBP htiw semit eerht desnir erew selpmas ehT Co73 ta h rof sllec eht htiw detabucni erew seidobitna yramirp ehT ylevitcepser ,PAFG rof 002 : dna ESN rof 002 : ta ares ... 001-X notirT %4.0 neht dna ,nim rof enoteca %001 ,nim 01 rof lonahtem %001 dloc eci htiw detaert neht erew yehT erutarepmet moor ta nim 01 rof SBP ni edyhedlamrof %7.3 htiw dexif neht dna SBP ... llec gnimrofrep dna esu lacinilc rof SBF gnisu tuohtiw oviv xe sCSM dnapxe ot sdohtem dnif ot deen ew dna ,hcraeser ruo rof SBF desu ew ,yduts tneserp ni ,revewoH noitarefilorp sCSM rof noitidnoc...
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Báo cáo y học: "The Short Anxiety Screening Test in Greek: translation and validation" ppsx
... contributed in the data collection and data entry, carried out the analysis, formed the layout of the manuscript and wrote the final manuscript AA carried out the statistical analysis and provided ... discrete data the c2 statistic For categorical data with more than two terms we used one-way analysis of variance (ANOVA) and in cases of statistical significance, a post hoc (Student-Newman-Keuls) analysis ... scope and the purpose of the study, and provided written consent Results Validation Study population A factor analysis was performed in order to determine the structure of the questionnaire and...
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Báo cáo y học: "Induction of HSPA4 and HSPA14 by NBS1 overexpression contributes to NBS1-induced in vitro metastatic and transformation activity" potx
... numerous candidates, we focused on two heat shock Figure NBS1 overexpression induced in vitro migration and invasion activity of H1299 cells and the expression of HSPA4 and HSPA14 (A) Western blot analysis ... Coomassie Brilliant Blue R-250 and then destained Transparent bands identified at 72 kD (latent form of MMP2) and 66 kD (active form of MMP2) on the Coomassie blue background of the gel were considered ... proteins, HSPA4 and HSPA14 siRNA mediated repression of HSPA4 or HSPA14 decreased the in vitro migration, invasion, and transformation activity of NBS1 overexpressing cells Induction of HSPA4 and HSPA14...
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báo cáo khoa học: " Characterization and isolation of a T-DNA tagged banana promoter active during in vitro culture and low temperature stress" ppt
... regions of tagged line ET2-17 and RT-PCR analysis of their regulation of the luc+ Cis-acting Cis-acting elements present in the 5'-tagged regions of tagged line ET2-17 and RT-PCR analysis of their ... 26°C (26) and the of the cultivar for RT-PCR 4analysis in untransformedand 8°C different 3'-tagged RT-PCR analysis in untransformed tissues of the cultivar 'Three Hand Planty' at 26°C (26) and 8°C ... cloning of tagged promoters, RT-PCR analysis and participated in sequence analysis as well as drafting the manuscript SR designed the experiments, participated in the data analysis and screening and...
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Báo cáo khoa học: "Tumor slices as a model to evaluate doxorubicin in vitro treatment and expression of trios of genes PRSS11, MTSS1, CLPTM1 and PRSS11, MTSS1, SMYD2 in canine mammary gland cancer" pptx
... chemotherapy and offers the advantage of an increased likelihood of breast conservation Many drug regimens have been used for a varied number of cycles and two of the most used drugs, doxorubicin and ... of the study, tissue slice culture PCR assays and revised the manuscript for important intellectual content HB participated in the design of the study and performed statistical analysis and data ... [13-15] Another potential benefit of neoadjuvant chemotherapy may be the opportunity of in vivo assessment of tumor response and the possibility of determination of potential predictive factors,...
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Engineering and characterization of human renal proximal tubular cells for applications in vitro toxicology and bioartifical kidneys
... improve my image analysis and compilation skills, Dr Karthikeyan Kandasamy for some of the qPCR experiments and Dr Rensheng Deng and Mohammed Shahrudin Ibrahim for providing the membranes and bioreactors ... Isolation of HPTC and characterization of isolated and commercial HPTC 50 4.1.1 Isolation of HPTC 50 4.1.2 Characterization of HPTC by immunoblotting 51 4.1.3 Characterization of ... Characterization of isolated and commercial HPTC 105 5.2 Characterization of effects of BMP-7 on HPTC and generation of a BMP-7producing BAK 107 5.3 Co-culture of HPTC with endothelial...
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Báo cáo y học: " Preparation of RGD-modified Long Circulating Liposome Loading Matrine, and its in vitro Anti-cancer Effects"
... successful coupling of the RGD to the surface of the liposomes Assay of particle sizes and morphological observation of RGD-M-LCL The average size of liposomes processed by the microfluidizer was ... means ± standard deviations, and samples were subjected to multiple analysis of variance The Dunnett-t test was performed to compare the mean between each test group and control group, and the ... RGD-LCL of 0.625 mg/mL), (D) matrine (0.0625 mg/mL), (E) RGD-LCL (1.25 mg/mL), and (F) RGD-M-LCL (equivalent to matrine of 0.0625 mg/mL and RGD-LCL of 1.25 mg/mL) Figure Representative results of...
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Analysis, sequencing and in vitro expression of PCR products
... intensity of the ethidium bromidestained band with the marker bands A total of 30–90 ng of DNA is recommended for direct sequencing of PCR products Analysis, sequencing and in vitro expression of PCR ... number of samples than the most direct approach; direct DNA sequence analysis of the PCR product (Section 5.4) Southern and dot blot analysis Southern blot analysis involves the transfer of DNA ... a combination of such factors (Chapter 4) In such Analysis, sequencing and in vitro expression of PCR products 89 cases it is often possible to increase the sensitivity of the analysis in order...
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Tài liệu Báo cáo khoa học: Proteolysis of Pseudomonas exotoxin A within hepatic endosomes by cathepsins B and D produces fragments displaying in vitro ADP-ribosylating and apoptotic effects doc
... analyses showed a progressive loss of intact ETA and ETA-A in the presence of ATP, with concomitant generation of ETA and ETA-A fragments Incubation in the Fig Assessment of ETA-degrading activity associated ... in the presence of [32P]NAD A low level of ADP-ribosylation of EF-2 was evident after addition of untreated ETA to the cytosolic fraction After treatment of ETA with cathepsins B and D, EF-2 labeling ... translocation of ETA into the cytoplasm of toxin-treated cells On the basis of the reconstitution of the cytotoxic pathways with in vitro cytosol and cell-free mitochondria, our data suggest a...
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Tài liệu Báo cáo khoa học: The endogenous retinoid metabolite S-4-oxo-9-cis-13,14-dihydro-retinoic acid activates retinoic acid receptor signalling both in vitro and in vivo pdf
... for either or 24 h for gene expression analysis and days for analysis of wing patterns Analysis of wing patterns and dissection of limb bud tissue After days of incubation, embryos were sacrificed, ... digit scored 33%; a digit of equivocal identity obtained a score of 0% For the analysis of limb buds, embryos were dissected out of the egg after and 24 h of incubation and rinsed in ice-cold NaCl ... soaking concentration and is a measure of the extent of pattern duplication (for definition, see Materials and methods) PRV is an average value of each set The sum of the scores of each wing is divided...
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Tài liệu Báo cáo khoa học: Template requirements and binding of hepatitis C virus NS5B polymerase during in vitro RNA synthesis from the 3¢-end of virus minus-strand RNA docx
... deletion mutants of the 3¢ terminus of the minus-strand RNA but deletions were made on the basis of the structure of the 5¢UTR of the plus-RNA, the structure of the 3¢-end of the minusstrand RNA being ... 3873 Binding and replication of 3¢-end of HCV minus RNA T Astier-Gin et al Fig Secondary structure of the 3¢ terminal sequences of HCV minus-strand RNA Model structures of domain I (A) and domain ... Binding and replication of 3¢-end of HCV minus RNA Fig Secondary structure of RNA mutated in the SL-A1 and SL-B1 domains Only the secondary structure of the 151 nt from the 3¢-end of the minus-strand...
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Tìm thêm: xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ chỉ tiêu chất lượng 9 tr 25