using stored procedures to query the database
Báo cáo y học: " Upregulated Genes In Sporadic, Idiopathic Pulmonary Arterial Hypertension" pdf
... used the PCRbased SSH method to identify upregulated genes in IPAH which may contribute to the disease process, lead to the discovery of the cause(s) of the disease and provide potential therapeutic ... Disabled homolog (Dab2) is a cystosolic cargo-specific adaptor protein, which binds to the cytoplasmic tails of lipoprotein receptors thereby selecting specific cargos at the plasma membrane during ... related to neurotensin and both bind the neurotensin-1 receptor [reviewed in [59]] In the lung, the neurotensin receptor is located mainly in fibroblasts [60] Whether xenin-25 is produced by the...
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Báo cáo Y học: Group IID heparin-binding secretory phospholipase A2 is expressed in human colon carcinoma cells and human mast cells and up-regulated in mouse inflammatory tissues doc
... and stained with hematoxylin and eosin to verify the progress of inflammation All procedures and analyses of other parameters are detailed elsewhere [40] Other procedures Northern and Western blottings, ... than 50% of the native enzyme (Fig 1C) The distribution of the KE2 mutant between the two fractions was similar to that of the native enzyme (Fig 1C) In contrast, the activity of the KE2RS2 mutant ... surfaces In the former situation, the glycosylphosphatidylinositol-anchored HSPG glypican supports the arachidonic acid-releasing function of the HSPG-binding sPLA2s by sorting them into particular...
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CURRENT BASIC AND PATHOLOGICAL APPROACHES TO THE FUNCTION OF MUSCLE CELLS AND TISSUES – FROM MOLECULES TO HUMANS pdf
... representing the position of the particle, were also taken to represent the position of the myosin head The position of the myosin head, determined by the above method, was compared between the two ... injected into the EC, and sucked out of the EC to keep the specimen in hydrated state (Fig.1B) In the research field of medical and biological sciences, it was a dream of investigators to observe ... to apply ATP to the specimen iontophoretically The image of the specimen is recorded with the imaging plate (IP) (Sugi et al , 1997) Current Basic and Pathological Approaches to the Function...
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Biopreservation of cells and engineered tissues
... owing to the potential use of these cells for immunotherapy The ability to preserve the phenotypic properties of a hematopoietic cell population and to manipulate the differentiation of the cells ... and the accumulation of harmful metabolites Hypothermic storage allows red blood cells that are preserved and stored for up to 42 days at ◦ C to be used in the treatment of anemic patients The ... organs has been used to extend the hypothermic storage time by supplying the necessary oxygenated preservation solutions that allow the organ to continue to function aerobically Using a modified UW...
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Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"
... modulate Treg cells to reduce the incidence of graft-versus-host disease In 80 addition, inhibition or restriction of these inflammatory mediators also alleviates further damage to the bone, cartilage ... Med Sci 2011, Then, the illuminator was turned off, and the image of light emitted from the nude mice, namely bioluminescence image, was taken Then, two images were merged and the location of ... dynamically monitored in a Kodak DXS small animal imaging system [10] When, the anesthetized nude mice were placed on the platform, the background image was taken under the lights of an illuminator http://www.medsci.org...
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HUMAN MILK OLIGOSACCHARIDES: CHEMICAL STRUCTURE, FUNCTIONS AND ENZYMATIC SYNTHESIS
... converted into UDPgalactose by GalE, and then β-1,4-GalT transfers galactose from UDP-galactose to acceptor (GlcNAc) to form N-acetyllactosamine (LacNAc, Gal-β-1,4-GlcNAc) LacNAc is then combined ... on the host’s epithelial cell surface (A), which is essential for bacteria to attach (a), and to proliferate and colonize the intestine (b) Some pathogens need to attach to the intestinal epithelial ... synthase NeuB, and the CMP-Neu5Ac synthetase NeuA The β-galactosidase gene lacZ was knocked out to prevent lactose hydrolysis and the nanA and nanK genes were knocked out to prevent the formation...
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Tài liệu Báo cáo khoa học: a-Defensins increase lung fibroblast proliferation and collagen synthesis via the b-catenin signaling pathway doc
... monitors the incorporation of BrdU into newly synthesized DNA The BrdU was detected using anti-BrdU-peroxidase conjugate in accordance with the manufacturer’s instructions After reactions were stopped, ... activate the b-catenin signaling pathway We then studied the role of the b-catenin signaling pathway in a-defensininduced increases in the proliferation and collagen synthesis of lung fibroblasts using ... regulatory protein in the Wnt cascade In nonstimulated cells, b-catenin is largely associated with cadherin There is very little b-catenin in the cytoplasm or nucleus because b-catenin in the cytoplasm...
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Tài liệu Báo cáo khoa học: Purification and cDNA cloning of a cellulase from abalone Haliotis discus hannai ppt
... Among the fragments, LP1-LP9 fractions were subjected to sequencing (Table 2) According to database searches on DDBJ, GenBank and EMBL, these sequences were found to show 42–80% identity to the ... not shown) The HdEG66 showed somewhat larger molecular size compared to the other invertebrate cellulases, however, the basic properties of the HdEG66 were fairly similar to those of other invertebrate ... attempting to purify these isoforms and determine their primary structures in order to reveal the structural characteristics of abalone cellulases and their evolutionary relationships to other invertebrate...
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Tài liệu Báo cáo Y học: Steady-state kinetics of the glutaminase reaction of CTP synthase from Lactococcus lactis The role of the allosteric activator GTP in coupling between glutamine hydrolysis and CTP synthesis potx
... rearrangements of the monomer that brings together the glutaminase site and the active site (C) GTP locks the enzyme in the active form for CTP synthesis and thereby stimulates the kcat of the reaction ... similar to that of the overall CTP synthesis reaction It was concluded that the effect of GTP was mainly to enhance the rate of chemical steps of the glutaminase reaction The nding that the kcat ... 7ị where KI is the concentration of inhibitor that gives rise to 50% inhibition by the inhibitor I and n is the Hillcoefcient The standard errors presented are those given by the computer program...
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Therapeutic ultrasound as a potential male contraceptive: power, frequency and temperature required to deplete rat testes of meiotic cells and epididymides of sperm determined using a commercially available system doc
... the bottom of the chamber to dissipate any heat built up in the transducer The transducer was affixed to an offset cam to allow it to rotate in a horizontal plane against the bottom of the ultrasound ... of the field output over time The distance between the transducer and the scrotum was initially set to cm In an attempt to increase the energy delivered to the testes, the distance between the ... from the bottom of the treatment chamber to aid positioning of the testes and to reduce reflection of ultrasound energy An ultrasound-transparent, nylon mesh was attached to the bottom of the...
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Báo cáo khoa học: Human skin cell stress response to GSM-900 mobile phone signals In vitro study on isolated primary cells and reconstructed epidermis docx
... at the ceiling of the cavity The dose absorbed by the irradiated object is constantly measured by a radiometer present in the apparatus, the measurement depending on the ageing of the lamp The ... been considered to be damaging to cells In the second phase, a series of experiments using NHDFc was performed to confirm the effect of RFR exposure on Hsc70 On the one hand, the Hsc70 expression ... according to time in culture second heat shock occurs after that period, the amount of HSP expressed during the first shock is sufficient to protect the cells during the second shock, so they not need to...
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Báo cáo khoa học: Characterization and cDNA cloning of a clofibrate-inducible microsomal epoxide hydrolase in Drosophila melanogaster potx
... reaching a peak at the wandering stage late in the last larval stadium At the prepupal stage, EH activity declined to a level equal to that in the early time of the stadium Northern blot analysis ... regulated at the transcriptional level Thus, the production of JHEH is regulated inversely to the JH titre It is not known whether JHEH is induced by xenobiotics or is involved in detoxification To elucidate ... in the total assay, respectively After the solvent was evaporated, the paper was wetted with 200 lL water in a 35 mm Petri-dish (Falcon) Ten larvae were allowed to crawl on the paper for h, then...
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Báo cáo Y học: Tyrosine sulfation and N-glycosylation of human heparin cofactor II from plasma and recombinant Chinese hamster ovary cells and their effects on heparin binding pot
... cDNA fragment into a HindIII/EcoRI-cleaved expression vector of the pSV2 plasmid series [19] The HCII cDNA spanned the protein coding sequence (including the signal peptide) down to the EcoRI site ... bubble-free aeration The device was placed on a stirring plate in a CO2 incubator together with a small membrane pump, which supplied the membrane stirrer with the incubator gas The starting volume ... Na] Even the linkage position of the fucose residue at the N-acetyllactosamine antennae could be assigned from the daughter ion spectra, due to the well-known preferential elimination of the 3-linked...
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Lecture 3 DNA RNA and protein synthesis great
... sequence codes The messenger RNA leaves the nucleus and attaches to a ribosome in the cytoplasm Transfer RNA brings amino acids to the ribosome to match the sequence codes At the ribosome, Amino ... Amino acids bond together and form polypeptides, Which bond together to make proteins Some examples of proteins are: Melanin, the pigment that gives our skin color Enzymes, the catalysts ... molecules found in the food we eat or produced in our cells It happens at the ribosomes DNA and protein synthesis, then, happens this way: DNA sequence codes (how letters are put together) produce...
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Stem CELLS and the FUTURE OF REGENERATIVE MEDICINE pdf
... or the brain, in the adult body They can renew themselves in the body, making identical copies of themselves for the lifetime of the organism, or become specialized to yield the cell types of the ... technology and to their use for the general welfare Upon the authority of the charter granted to it by the Congress in 1863, the Academy has a mandate that requires it to advise the federal government ... organ in the hope that they will give rise to the mature cells needed by that organ In the second, the stem cells are stimulated to differentiate into the needed mature tissue outside the body,...
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Báo cáo khoa học: Complex gangliosides are apically sorted in polarized MDCK cells and internalized by clathrin-independent endocytosis doc
... sections (xy, 0.2 lm) collected from the top to the bottom of the cell monolayer Then, xz sections were displayed using the ortho mode in LSM5 PASCAL software The bottom rows in each set of images ... cell lines were monitored using a specific antibody to GD3 and CTx, respectively Therefore, the results described in this work provide the basis to gain further insight into the molecular mechanisms ... belonging to series ‘o’, by catalysing the transfer of N-acetyl-d-galactosamine to the galactose residue of LacCer As observed previously in other cell lines [9,41], it is expected that the synthesis...
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Định lượng mô, tế bào bằng phân tích hình ảnh (Quantifying tissues, cells by image anaslysis) potx
... máy vi tính theo chơng trình phần mềm xử lý hình ảnh để tăng cờng chất lợng ảnh (tăng độ tơng phản, xác định ranh giới, tăng giảm mầu sắc); tín hiệu số máy tính đợc tự động điều chỉnh theo Có cách ... đối tợng Có tham số huỳnh quang hay đợc khai thác, là: Tham số phát huỳnh quang to n phần (tổng cờng độ phát quang to n bề mặt đối tợng) Tham số phát huỳnh quang trung bình (cờng độ phát quang ... York Weibel, E R (1969) Stereological principles for morphometry in Electronmicroscopy cytology Int Rev Cytol 26, 235 - 302 John J Bozzola and Lonnie D Russell (1990) Quantitative Electron Microscopy...
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simple and rapid synthesis of a-fe2o3 nanowires under ambient conditions
... of the growing NW is surface diffusion The sword-like shape of the NWs confirms that the growth is determined by a diffusion process from the bottom—where the NWs are thicker to the top, where they ... temperature to the optimum synthesis temperature allowed us to determine the maximum NW growth rate For this purpose, we applied a potential difference to wires (to heat them up to 700 °C) for ... controlled by observing the color of the wire and by varying the applied heating power (see the ESM) SEM observation of the wire after the synthesis of the reddish material revealed that the wire was completely...
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Báo cáo khoa học: LRRK2 in Parkinson’s disease: function in cells and neurodegeneration doc
... pro-apoptotic protein Activated Bid translocates to the mitochondria, where it signals the formation of BAX-BAK oligomers into a proteolipid pore Cytochrome c and other factors are released from the ... space into the cytosol, where cytochrome c activates apoptosome formation Initiator caspase-9 undergoes a conformational change, activating photolytic activity The apoptosome cleaves effector caspase-3, ... may serve as a scaffold for the recruitment of FADD together with TRADD, tumor necrosis factor receptor associated factor-2 and RIP1 in the formation of complex II The specific LRRK2 domain that...
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Báo cáo khoa học: Prohibitin is expressed in pancreatic b-cells and protects against oxidative and proapoptotic effects of ethanol pdf
... °C, for to remove nuclear and cell debris The resulting supernatant was subsequently centrifuged at 5500 g, °C, for 10 to yield the mitochondrial fraction The mitochondrial fraction was stored ... construct, this fusion protein moved from the cytoplasm into the mitochondria and inhibited apoptosis In a more recent study, Theiss et al [24] reported that in inflammatory bowel diseases, PHB localizes ... blot of PHB in the cytoplasmic fraction (n = experiments) (F) Endogenous PHB protein in the mitochondrial fraction, expressed as the mean ± SEM arbitrary units relative to the mitochondrial marker...
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Tìm thêm: xác định các mục tiêu của chương trình khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25