use of vaccines to induce long term alterations in innate subsets functions
Báo cáo y học: " Revisiting HIV-1 uncoating" doc
Ngày tải lên :
13/08/2014, 01:20
... determines their capacity to integrate into chromatin Retrovirology 2006, 3:4 30 Farnet CM, Haseltine WA: Determination of viral proteins present in the human immunodeficiency virus type preintegration ... an increasing appeal to determine up to which point the viral capsid is required for infection and at which point in space and time it is disassembled Uncoating accompanies the conversion of ... stability are key to retroviral infections In the case of restriction of HIV-1 in rhesus macaque cells, it was originally supposed that TRIM5a binding might inhibit the uncoating of the viral capsid...
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Nghiên cứu xây dựng quy trình phát hiện nhanh virus cúm gia cầm a h5n1 trong mẫu bệnh phẩm bằng kỹ thuật multiplex reverse transcription polymerase chain reaction
Ngày tải lên :
10/02/2014, 20:39
... Emerg Infect Dis, 10, pp 1321- Baigent SJ, McCauley JW (2001), “Glycosylation of haemagglutinin and stalk-length of neuraminidase combine to regulate the growth of avian influenza viruses in tissue ... (2008), “Review of clinical symptoms and spectrum in humans with influenza A/H5N1 infection”, Respirology, 13(suppl 1):S10-S13 32 International Committee on Taxonomy of Viruses Index of Viruses (2009), ... diversity of H5N1 influenza virus in China, 1996-2006”, Virology, 380, pp 243-54 23 Gambaryan A, Tuzikov A, Pazynina G, et al (2006), “Evolution of the receptor binding phenotype of influenza...
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Báo cáo khoa học: Ribonuclease H: properties, substrate specificity and roles in retroviral reverse transcription ppt
Ngày tải lên :
30/03/2014, 02:20
... the C-terminal 25% of the protein, with the connection domain accounting for the remainder HIV-1 reverse transcriptase is a heterodimer made up of a p66 subunit containing the active forms of ... 20583–20585 13 Hostomsky Z, Hostomska Z, Hudson GO, Moomaw EW & Nodes BR (1991) Reconstitution in vitro of RNase H activity by using purified N-terminal and C-terminal domains of human immunodeficiency ... the pairing of the complementary PBS and PBS¢ sequences A combination of nondisplacement and displacement synthesis [102] converts the circular intermediate into the final linear product of reverse...
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Báo cáo sinh học: " Quantitative assessment of the effect of uracil-DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription-PCR" pdf
Ngày tải lên :
19/06/2014, 08:20
... would cause false positives in PCR or RT-PCR UNG degrades contaminating uracil-containing DNA while leaving the natural, thymine-containing DNA intact The precise, reproducible quantification of realtime ... extraction Extraction of Porcine arterivirus RNA from cell culture stocks of the vaccine strain Ingelvac MLV (derived by serial passage of U.S prototype strain VR-2332) was performed using the Qiagen ... reactions containing UNG To determine if UNG addition and incubation prior to RTPCR would result in false negative results under the conditions described, 96 replicate samples containing approximately...
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Báo cáo hóa học: " A broad spectrum, one-step reverse-transcription PCR amplification of the neuraminidase gene from multiple subtypes of influenza A virus" pdf
Ngày tải lên :
20/06/2014, 01:20
... viruses from a range of Mammalian and Avian species The sequence information obtained can be helpful in determining the origin of the influenza virus and can be interrogated for the presence of ... pathogens of the three types and have been associated with all of the past influenza pandemics [4,5] Influenza A viruses are classified into subtypes according to the hemagglutinin (HA) and neuraminidase ... strain H3 H3N2 Wisconsin/67/2005 like strain H3N2 Wisconsin/67/2005 like strain H3N2 Wisconsin/67/2005 like strain H3N2 Wisconsin/67/2005 like strain H3 H3 H3N2 Wisconsin/67/2005 like strain H3N2...
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báo cáo hóa học:" Quantitative assessment of the effect of uracil-DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription-PCR" ppt
Ngày tải lên :
20/06/2014, 04:20
... would cause false positives in PCR or RT-PCR UNG degrades contaminating uracil-containing DNA while leaving the natural, thymine-containing DNA intact The precise, reproducible quantification of realtime ... extraction Extraction of Porcine arterivirus RNA from cell culture stocks of the vaccine strain Ingelvac MLV (derived by serial passage of U.S prototype strain VR-2332) was performed using the Qiagen ... reactions containing UNG To determine if UNG addition and incubation prior to RTPCR would result in false negative results under the conditions described, 96 replicate samples containing approximately...
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Báo cáo hóa học: "Rapid detection of sacbrood virus (SBV) by one-step reverse transcription loop-mediated isothermal amplification assay" pptx
Ngày tải lên :
21/06/2014, 19:20
... honeybee viruses To evaluate the application of RT-LAMP to detect SBV in clinical samples, the test was performed on 30 field clinical samples collected in Chongqing, China, in the period March to May ... RT-LAMP protocol was therefore determined to be for a time of between 30 and 50 at a temperature in the range 63°C–65°C Figure Determination of the optimal time of the LAMP Lane M, DL-2000 DNA ... Probes, Inc.) The solution changed from light orange to green in the presence of LAMP amplicons, while it remained light orange in the absence of amplification Amplified DNA in the LAMP reaction causes...
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Báo cáo khoa học: "TaqMan reverse transcription polymerase chain reaction for the detection of Japanese encephalitis virus" pot
Ngày tải lên :
07/08/2014, 18:20
... no template control in B KV1899 strain were cultured in TF104 cells, while Nakayama strain was obtained from brain emulsion of the inoculated mouse When several reference viruses were screened ... RNA including RNase inhibitor, RNAsin (Promega, USA) was stored at −70oC until use Selection of primers and probes Three different primers and probes were designed based on conserved regions of ... real-time RT-PCR method could be used to detect and quantify JEV concentration in pigs for the purpose of determining JEV viremia Forty one fetuses that aborted before 70 days of gestation also were tested...
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Báo cáo khoa học: "Development of a Lightcycler-based reverse transcription polymerase chain reaction for the detection of foot-and-mouth disease virus" pps
Ngày tải lên :
07/08/2014, 18:21
... citsongaid rof sdradnats fo launaM seitoozipE seD lanoitanretnI eciffO 17 -36 ,92 ,4002 seneG suriV sniarts aisAnaP rehto dna aeroK ni slamina morf sesuriv esaesid htuom-dna-toof fo ecneuqes editoelcun ... elbaT( derapmoc erew stluser eht dna ,demrofrep erew RCP-TR lanoitnevnoc dna RCP-TR T /R ,selpmas gnaborP ni ANR VDMF fo noitceted eht roF )nwohs ton atad( smetsys RCP-TR htob rof seiticificeps lacitnedi ... htuom-dna-toof fo sepytores lla fo noitceted suoenatlumis eht rof yassa RCP -TR T/R evitatitnauq levon a fo tnempoleveD T draagrotS ,S kaleB ,K rekcirtS eD ,A lahtnettU ,BT nessumsaR 4002 ,siraP ,seotoozipE...
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Báo cáo y học: "Investigation of infectious agents associated with arthritis by reverse transcription PCR of bacterial rRNA" ppsx
Ngày tải lên :
09/08/2014, 01:21
... one out of three patients with ReA thought to be related to Yersinia infection had evidence of the specific rRNA in SF The findings from investigations in patients with Campylobacter-induced ... (including the E coli sequences), arguing against laboratory contamination Together, this constitutes strong evidence of the ability of commensal organisms such as E coli to colonize inflamed joints; ... specificity (Yersinia and Chlamydia rRNAs were only detected in patients in whom other evidence pointed to the involvement of these organisms) but low sensitivity in the case of Yersinia, because only...
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Báo cáo khoa hoc:" Quantitative competitive reverse transcription polymerase chain reaction is not a useful method for quantification of CD4 and CD8 cell status during HIV infection" pptx
Ngày tải lên :
11/08/2014, 08:20
... type PCR product was inserted into the TA cloning vector before digesting with BbsI, an enzyme with no recognition sites inside the vector and only one site in the insert The linearized plasmid ... cDNA/competitor band intensity (as determined by analysis of PICT files with NIH image) on the x-axis versus the log of incremental dilutions of competitor DNA on the y-axis Solving for the antilog of ... JD High levels of HIV-1 in plasma during all stages of infection determined by competitive PCR Science 1993, 259(5102):1749-1754 McKinney RE Jr and Wilfert CM Lymphocyte subsets in children younger...
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Báo cáo y học: " Variability in a dominant block to SIV early reverse transcription in rhesus monkey cells predicts in vivo viral replication and time to death" pot
Ngày tải lên :
12/08/2014, 04:20
... resistant to SIV infection (Fig 6) These data suggest that a dominant factor exists in resistant B-LCL that can inhibit SIV replication To determine if a protein or variable forms of a protein inhibit ... observed an increase in SIVsmE660 replication in resistant cell lines as we added increasing quantities of VLPs In fact, the addition of 150 ng of VLPs led to the complete loss of resistance to SIV ... Following infection of rhesus monkey B-LCL with this construct, we observed a broad range in B-LCL susceptibility to SIV infection, varying from 1% to 30% of target cells infected (Fig 2A) To determine...
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Báo cáo y học: " A multiplex reverse transcription-nested polymerase chain reaction for detection and differentiation of wild-type and vaccine strains of canine distemper virus" pptx
Ngày tải lên :
12/08/2014, 04:20
... different farms in Heilongjiang and Jilin provinces of China RT-nPCR was used to detect the cells infected with CDV vaccine strain, wild-type strain, mixed CDV vaccine and wild-type strains, CPV, CAV, ... Hill (AF259552) at known receptor-binding sites of the haemagglutinin gene is associated with independent instances of the spread of CDV to novel non-dog hosts in the wild The selected two samples ... of a number of CDV strains available in GenBank using the MegAlign function of the DNAStar software package Thirty field samples from dogs, foxes, and raccoons in Heilongjiang and Jilin provinces...
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Retrovirology Review BioMed Central Open Access When is it time for reverse transcription to docx
Ngày tải lên :
12/08/2014, 23:20
... indications that the conserved CCHC zinc fingers of NC contribute to the maintenance of the complete viral DNA and its IN- mediated integration into the host genome during the course of cell infection ... mechanisms of such an enhancement in virus infectivity remain to be determined A further important avenue of research involves the characterization of compounds aimed at inhibiting HIV-1 RTNC interactions, ... [12,14] The zinc fingers and the RT palm domains are probably required for NC-RT recognition [42-45,81]; yet, the exact interacting domains remain to be determined in vitro and in the viral context...
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Báo cáo y học: " The RNA binding protein HuR does not interact directly with HIV-1 reverse transcriptase and does not affect reverse transcription in vitro" doc
Ngày tải lên :
12/08/2014, 23:23
... assembly of HuR oligomers on RNA [17] In addition, RRM of HuR was reported by Lemay et al to directly interact with the RNase H domain of HIV-1 RT [9] To further define the structural determinants of ... characterization of HuR HuR belongs to the Hu family of mRNA stabilizing proteins that interact with AU-rich elements (ARE), sharing Page of significant sequence similarity with the Drosophila RNAbinding ... domains are depicted by boxes and the numbers refer to amino acid positions in the full length proteins The putative binding site for HIV-1 RT is indicated by the arrow at the C-terminus of HuR...
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Báo cáo y học: "Subtype-associated differences in HIV-1 reverse transcription affect the viral replication" ppt
Ngày tải lên :
13/08/2014, 01:20
... incorporation of viral RNA into the virions and processing of GagPol polyprotein-precursor in the virus particles to test the potential effect of the subtype C protease and C-terminal domains of Gag in ... N-terminal portion of RT together with the C-terminal Pol domains, the Vif and probably the Vpr proteins may contribute to the lower replication level of the subtype C viruses To further determine ... minus-strand strong-stop DNA synthesis [25] Since the RNase H domain has been shown to profoundly affect the functions of the polymerase domain [55,56], our findings suggest that the C-terminal...
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Báo cáo y học: " Blocking premature reverse transcription fails to rescue the HIV-1 nucleocapsid-mutant replication defect" ppsx
Ngày tải lên :
13/08/2014, 01:20
... preparations Initial experiments were performed to determine the necessary concentrations of RTIs to use and we found that a single inhibitor was insufficient to block the levels of premature ... range) Interestingly, because PMPA is a chain terminator, it functions by being incorporated into the nascent vDNA and thus would not be affected by reducing its concentration in the media However, ... line) However, the increase in infectivity is directly proportional to the increase in the amount of NCWT We see a slightly different result with viruses containing different ratios of NC WT to...
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Báo cáo y học: "Isolated HIV-1 core is active for reverse transcription" pptx
Ngày tải lên :
13/08/2014, 05:22
... treatment to remove the viral envelope would appear to be counterintuitive Interestingly, in support of our data, capsid protein has been reported in dense fractions of virions subjected to equilibrium ... block polymerase elongation; however, we have no data as to the effect of elongation on core structure and it could be that the elongation of the polymerase results in shedding of capsid as suggested ... 1:9 Goff SP: Intracellular trafficking of retroviral genomes during the early phase of infection: viral exploitation of cellular pathways J Gene Med 2001, 3:517-28 Accola MA, Ohagen A, Gottlinger...
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Báo cáo y học: "Early and transient reverse transcription during primary deltaretroviral infection of sheep" ppt
Ngày tải lên :
13/08/2014, 06:20
... during the synthesis of the 5' RU5 minus strand As shown in lines to 6, this substitution appears to be harbored by both strands of the two LTRs of the integrated provirus Accordingly, all infected ... Tajima S, Tsukamoto M, Aida Y: Latency of viral expression in vivo is not related to CpG methylation in the U3 region and part of the R region of the long terminal repeat of bovine leukemia virus ... 60°C min, 72°C min), and a final elongation step of 10 at 72°C The length polymorphism analysis of 3' BLV flanking sequences was performed by making a run-off This method consists in the linear...
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Báo cáo y học: " Contribution of the C-terminal tri-lysine regions of human immunodeficiency virus type 1 integrase for efficient reverse transcription and viral DNA nuclear import" pps
Ngày tải lên :
13/08/2014, 09:21
... [17] in the C-terminal domain of HIV-1 IN, impaired protein nuclear localization, while mutations of arginines at amino acid position of 263 and 264 in the distal part of the C-terminal domain of ... tri-lysine regions in the C-terminal domain of IN are involved in the protein nuclear localization The C-terminal domain of HIV-1 IN contains several regions that are highly conserved in different ... feature of HIV-1 IN To delineate the karyophilic determinant in HIV-1 IN, two truncated IN- YFP expressors CMV -IN5 0–288-YFP and CMVIN1–212-YFP were generated In CMV -IN5 0–288-YFP, the Nterminal HH-CC...
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