use of ocular micrometer measure of relative amp absolute cell size
modern neuroscience research protocol
Ngày tải lên :
11/04/2014, 09:51
... Examples of staining of single neurons by intracellular iontophoresis of horseradish peroxidase Purkinje cells of the cerebellar cortex of the cat A: Reconstruction of the collateral branches of ... (A), 50 µm (Β-F) a, axon of Purkinje cell (B), basket cell (D), Golgi cell (E), or granule cell (F); b, basket; bc, basket cell; Bg, Bergmann glial cell process; gc, granule cell; gl, granular layer; ... contact of a branch of C with a deep nuclear cell in more detail D: Diagram of a sagittal section of the cerebellum showing the sites of foliar origins and deep nuclear terminations of Purkinje cells...
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Báo cáo hóa học: " A broad spectrum, one-step reverse-transcription PCR amplification of the neuraminidase gene from multiple subtypes of influenza A virus" pdf
Ngày tải lên :
20/06/2014, 01:20
... amplification of NA gene from clinical NPA samples Example of amplification results of Influenza A and influenza B Sample numbers correspond to the samples described in Table 1, except sample 35 which ... One of these samples had visible discoloration which could indicate the presence of compounds that may interfere with the sequencing process One of the panels of N6 viruses (H13N6) failed to amplify ... 2008, 5:77 Figure Sensitivity of the one-step RT-PCR assay Sensitivity of the one-step RT-PCR assay Example of amplification results of ten-fold serial dilutions of in-vitro transcribed RNA H10N7...
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Primer design for the PCR amplification of the coad gene
Ngày tải lên :
14/07/2015, 23:53
... this 5'-end primer will not result in the amplification of the original coaD gene The use of the Nco I restriction site dictates what the first nucleotide of the next triplet codon must be (G) In ... strategy which will result in the amplification of the original gene is described in Utilisation of the Nco I site 3'-end primer No C-terminal tag is being used, so we have to add a stop codon ... (CG) BamH I restriction site (GGATCC) Complement of stop codon (CTA) Overlap with the strand complement to the 3'-end of the coaD gene Estimated Tm of 60°C [8 * (A+T) + 11 * (C+G)] GC content is...
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Tài liệu Báo cáo khoa học: PCR detection of nearly any dengue virus strain using a highly sensitive primer ‘cocktail’ ppt
Ngày tải lên :
14/02/2014, 19:20
... ⁄ 36 cells showed amplification, and only in the presence of RT No amplification was observed with total RNA of normal C6 ⁄ 36 A albopictus cells or in the absence of template DENV-2 was amplified ... albopictus) cells and uninfected C6 ⁄ 36 mosquito cell samples were generously provided by R B Tesh, Director of the World Reference Center for Emerging Viruses and Arboviruses at the University of Texas ... mosquito cells cDNA was synthesized directly using the primers described in Table In a 25 lL PCR reaction, either 100 pg of DENV-1-, ng of DENV-2-, 100 pg of DENV3- or ng of DENV-4-infected C6 ⁄ 36 cells...
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Báo cáo khoa học: Semi-nested PCR analysis of unknown tags on serial analysis of gene expression potx
Ngày tải lên :
07/03/2014, 04:20
... a primary library of spermatozoa cDNAs to be used for further studies Amplification of primary library The full-length cDNAs in spermatozoa were amplified by PCR with the use of Takara Ex Taq Hot ... corresponding to tags could be amplified 3530 bp 1584 bp 947 bp 564 bp Amplification of longer sequences from SAGE tags Fig PCR amplification of the full-length cDNAs The cDNAs were amplified with PLF and ... mechanism of the amplification of the whole cDNAs and the TSAT-PCR technique (A) In this process, double-stranded cDNAs synthesized by modified lock-docking oligo(dT) and 5¢-cap oligonucleotides were used...
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pcr detection of microbial pathogens
Ngày tải lên :
11/04/2014, 10:01
... homogeneity of the sample may differ depending on the kind of biological sample matrices If components of the sample matrix have the same density as the cells these may inhibit DNA amplification ... optimization of the DNA amplification conditions by the use of alternative DNA polymerases, and/or amplification facilitators; and (iii) a combination of both strategies Selection and optimization of sample ... of biological samples prior to diagnostic PCR Sample Preparation The objectives of sample preparation are (i) to exclude PCR-inhibitory substances that may reduce the amplification capacity of...
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Báo cáo sinh học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pdf
Ngày tải lên :
18/06/2014, 18:20
... the effects of viruses on host cell gene expression as viruses can interfere with host cell pathways, the components of which traditional housekeeping genes often encode These include cell cycle, ... a variety of cell types infected with a variety of strains of HIV-1, HSV-1, VZV and cytomegalovirus (CMV) compared to mock infected cells Results A range of cell lines and primary cells were ... the herpes viruses, herpes simplex virus (HSV) and varicella zoster virus (VZV) Thus, in this study we have investigated the suitability of 10 commonly used housekeeping genes for use as QPCR normalisers...
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Báo cáo sinh học: " A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame" potx
Ngày tải lên :
19/06/2014, 08:20
... ~2.8kb Figure position of amplicon amplification primers Relative Relative position of amplicon amplification primers Three pairs of amplification primers and their relative positions are shown The ... time of 2.5 was used for amplicons over kb (amplicons 1, and 3), and extension time of was used for amplicons less than kb (amplicons 1x, 1y, 4x and 4y) A PCR hood and aerosol-barrier tips were used ... http://www.virologyj.com/content/2/1/88 Amplicon Amplicon 1364 57 4995 7698 1x 1y 1313 2566 ORF 5’UTR 2407 5026 7283 8373 3’UTR 4x 4y 8288 Amplicon 9524 Amplicon Figure HCV genotype 1b amplicons HCV genotype 1b amplicons Amplicons...
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Báo cáo hóa học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pot
Ngày tải lên :
20/06/2014, 01:20
... the effects of viruses on host cell gene expression as viruses can interfere with host cell pathways, the components of which traditional housekeeping genes often encode These include cell cycle, ... a variety of cell types infected with a variety of strains of HIV-1, HSV-1, VZV and cytomegalovirus (CMV) compared to mock infected cells Results A range of cell lines and primary cells were ... the herpes viruses, herpes simplex virus (HSV) and varicella zoster virus (VZV) Thus, in this study we have investigated the suitability of 10 commonly used housekeeping genes for use as QPCR normalisers...
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báo cáo hóa học:" A general method for nested RT-PCR amplification and sequencing the complete HCV genotype 1 open reading frame" pdf
Ngày tải lên :
20/06/2014, 04:20
... ~2.8kb Figure position of amplicon amplification primers Relative Relative position of amplicon amplification primers Three pairs of amplification primers and their relative positions are shown The ... time of 2.5 was used for amplicons over kb (amplicons 1, and 3), and extension time of was used for amplicons less than kb (amplicons 1x, 1y, 4x and 4y) A PCR hood and aerosol-barrier tips were used ... http://www.virologyj.com/content/2/1/88 Amplicon Amplicon 1364 57 4995 7698 1x 1y 1313 2566 ORF 5’UTR 2407 5026 7283 8373 3’UTR 4x 4y 8288 Amplicon 9524 Amplicon Figure HCV genotype 1b amplicons HCV genotype 1b amplicons Amplicons...
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Báo cáo y học: "Selective amplification of glucocorticoid anti-inflammatory activity through synergistic multi-target action of a combination drug" ppsx
Ngày tải lên :
09/08/2014, 01:22
... Institutional Animal Care and Use Committee Corticotroph cAMP assay AtT-20 cells were seeded at a density of 60,000 cells per well in a 96-well plate for determination of changes in cAMP levels in response ... vitro of the POMC gene and suppression of secreted ACTH from corticotroph cells The effects of prednisolone, dipyridamole, and the combination were measured in the murine anterior pituitary cell ... molecular context of cellular networks that mediate glucocorticoid adverse effects (for example, corticotrophs or hepatocytes) not support amplification, and therefore the safety profile of the very-low-dose...
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Báo cáo lâm nghiệp: "Amplification of root—fungus interface in ectomycorrhizae by Hartig net architecture" pot
Ngày tải lên :
09/08/2014, 04:20
... delineated from micrographs of the Hartig net and from possible alternatives in growth of hyphae (Fig 3) Area, perimeter and length of hyphal walls of the models were measured with Mop-Videoplan, ... hyphae Measurements from these models of area and perimeter of hyphal complex and length of hyphal walls are presented in Table I (Trinci, 1984; Prosser, 1983) The result is pinnate growth of scattered ... system (Zeiss-Kontron), using standard software The surface/volume ratios of the different systems were calculated on the basis of an average pm diameter of hyhae Results frequently be found separated...
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Báo cáo y học: "Amplification of autoimmune disease by infection" potx
Ngày tải lên :
09/08/2014, 06:22
... dendritic cells [33,34] and endothelial cells, and may establish a latency reservoir in these cells too [30] Lytic infection can involve epithelial cells, fibroblasts, stromal cells, neuronal cells, ... cells, B cells, natural killer cells (viral subgroup A) and dendritic cells [45] Finally, lytic infection can occur in many other cell types including neurons, muscle cells and epithelial cells ... predominantly T cells but also myelomonocytic cells [49–51] Like other herpesviruses it can infect epithelial and endothelial cells Salivary glands are a major site of production of HHV7 [9,52]...
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Báo cáo khoa học: "Real time PCR analyses of expression of E-cadherin, alpha-, beta- and gamma-catenin in human breast cancer for predicting clinical outcome" pps
Ngày tải lên :
09/08/2014, 07:21
... expression of E-cadherin, α-, β-, and γ-catenin The expression of the various molecular markers in previous studies has been assessed by immunohistochemical staining in a small sample size The use of ... allows real-time detection of 96 reactions was used to amplify the plasmid standards and breast tissue samples under the following conditions: 94°C for 12 minutes; 50 cycles of 94°C for 15 s, 55°C ... development stage of primary tumours This loss of adhesiveness at primary site of tumours allows cancer cells to 'dissociate' from each other However, following invasion and degradation of surrounding...
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Báo cáo y học: "Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries" pps
Ngày tải lên :
09/08/2014, 22:23
... sample was divided by the mean quantity of the two amplicons closest to 50% GC The resulting relative abundances of amplicons were plotted on a log10 scale over their respective GC contents Relative ... and size- selected to approximately 360-bp instead of 180-bp inserts The GC profiles of PCR-amplified largerinsert libraries were almost as flat as that of a small-insert control library amplified ... quantities of the 48% and 52% GC amplicons in the same sample Hence, all GC-bias plots meet at 50% GC For human-derived samples, we divided the average quantity of each amplicon by the mean quantity of...
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Báo cáo y học: " Monitoring human cytomegalovirus infection with nested PCR: comparison of positive rates in plasma and leukocytes and with quantitative PCR" doc
Ngày tải lên :
12/08/2014, 04:20
... irrespective of their reported detection limits Another limitation of this study is that the sample size was relatively small; nevertheless, this study contained prospective serial samples in which ... kit contains reagents and enzymes for the specific amplification of a 86-bp region of the IE1 gene of HCMV For extraction of DNA, an aliquot of 400 μl EDTA-anticoagulated blood was collected and ... Calculations of Ct, preparation of standard curve and quantification of each sample’s DNA were performed by the Option Monitor Software According to the manufacture’s instructions, the detection limit of...
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Báo cáo khoa học: " Nested-multiplex PCR detection of Orthopoxvirus and Parapoxvirus directly from exanthematic clinical samples" pdf
Ngày tải lên :
12/08/2014, 04:20
... The sequencing of vgf and b2l amplicons confirmed the specificity of the nested-multiplex approach The sensitivity and Table 2: Clinical samples used to evaluated the performance of the OPV/PPV ... all analyzed CE clinical samples Considering all bovine, human, ovine and caprine samples, the nested-multiplex PCR presented a positivity of 95.3% The sequences of the amplified fragments again ... expected fragments of 170 bp (OPV) and 592 pb (PPV)], described as follows In the first step, μL of template were added to 18 μL of the PCR reaction mixture containing 0.4 mM of OPV primers (VGF-F...
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Báo cáo sinh học: "Rapid PCR detection of group a streptococcus from flocked throat swabs: A retrospective clinical study" ppsx
Ngày tải lên :
12/08/2014, 17:20
... change of the sample in a temperature range of 60°C to 90°C The derivative of the measured fluorescence plotted against temperature was displayed using the HR-1 software and the peak of these ... dilutions of bacterial reference strains has shown improved sensitivity for nucleic acid amplification assays using flocked swabs [10].) This assay also provides another example of the usefulness of ... of each primer, a picomolar concentration of internal control oligonucleotide and μL of specimen DNA The thermocycler (RapidCycler II, Idaho Technology Inc.) protocol consisted of 40 cycles of...
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Báo cáo y học: "Serologic and PCR testing of persons with chronic fatigue syndrome in the United States shows no association with xenotropic or polytropic murine leukemia virus-related viruses" pot
Ngày tải lên :
13/08/2014, 01:20
... developed from mouse hybridoma cells, such as the Platinum Taq used by Lo et al [22] While Lo et al did not find mouse cell contamination by a retrospective screen of their samples for murine ... consisting of 101 and 103 copies of XMRV VP62 plasmid DNA diluted in a background of μg of human PBMC DNA, respectively Figure Absence of XMRV/MuLV sequences by real-time PCR in PBMC DNA of persons ... PBMC DNA used by Lombardi et al [5,14] Dilutions of DNA from XMRV-infected 22Rv1 human prostate carcinoma cells were used as positive controls in this test [15] 1.0 μg of DNA (333 ng of PBMC DNA)...
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Báo cáo sinh học: "Long telomeres in the polytene chromosomes of Drosophila melanogaster are associated with amplification of subtelomeric repeat sequences" doc
Ngày tải lên :
14/08/2014, 20:20
... estimation of the number of copies of Dm665 that occur in the tips of metaphase chromosomes in diploid tissues The effect of temperature on the morphology and pattern of hybridization of telomeres ... We conclude that the morphological changes in the telomeres of polytene chromosomes are caused by different degrees of amplification of at least Dm665 and perhaps other telomereassociated sequences ... Analysis of specificity of ectopic conjugation of telomeric ends of Drosophila melanogaster polytene chromosomes Genetika (USSR) 22, 787-792 Renkawitz-Pohl R, Bialojan S (1984) A DNA sequence of Drosophila...
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