unglycosylated does not form oligomers and is not located to the cell surface
Báo cáo y học: "Variations in autologous neutralization and CD4 dependence of b12 resistant HIV-1 clade C env clones obtained at different time points from antiretroviral naïve Indian patients with recent infection" doc
... sensitivities to their autologous plasma antibodies and differed in their susceptibilities to MAbs, indicating distinct mechanisms of autologous neutralization and antibody specificities in these patients ... resistant to IgG1b12 This observation of b12 resistance of the India clade Envs is in line with that reported by Kulkarni et al [27] As with the MPER-specific MAbs, all the Envs were resistant to ... Envs between IVC and 4, IVC and 11, IVC and and IVC and 11 their sensitivity to sCD4 and contemporaneous plasma antibodies Thus, Envs 2-3.J18, 2-5.J3 and 2-5.J11 which showed the highest neutralization...
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Báo cáo khoa học: Sensitivity to Hsp90-targeting drugs can arise with mutation to the Hsp90 chaperone, cochaperones and plasma membrane ATP binding cassette transporters of yeast docx
... resistance (Pdr5p and Snq2p [46]) were streaked onto plates containing GA and RD This revealed the Dpdr5 mutant to be hypersensitive to both drugs and the Dsnq2 mutant to be slightly sensitive to ... complex To seek evidence for whether this is the case, we determined if, through the expression of the T101I and A598T mutant forms of Hsp82, an Hsp90dependent process becomes more sensitive to Hsp90 ... since found these effects of the sti1D mutation on drug sensitivity to be influenced strongly by the isoform of Hsp90 that is expressed (at a similar level) in the cells Increased GA and RD sensitivity...
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Arsenic speciation in environmental and biological samples extraction and stability studies
... as fish, chicken and rice, and in environmental samples It is well known that fish and shellfish have the ability to bioaccumulate the non-toxic AsB while other food, such as rice, is a bioaccumulative ... paramount importance [16,17] On the other hand, it is also important to know the arsenic species stability in the extracts under several storage conditions, due to the possible high lapse of time ... decreased with time To validate the analytical methodology, the total As content and As species were quantified in the CRMs used, NIST 1558a (rice flour) and NRCC Dorm-2 (dogfish muscle) The total As content...
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Báo cáo khoa học: An estrogen receptor a suppressor, microRNA-22, is downregulated in estrogen receptor a-positive human breast cancer cell lines and clinical samples pptx
... plot The line inside each box is the median; the upper and lower limits of the box are the 75th and 25th percentiles, respectively, and the vertical bars above and below the box indicate the maximum ... surgery and they signed informed consent forms for sample collection ERa protein expression status was confirmed at diagnosis using standard immunohistochemistry procedures Total RNA was isolated and ... MDA-MB-231 and P = 0.0010 for the comparison between BT-474 and MDA-MB231, but P = 0.0399 for the comparison between MCF-7 and SK-BR-3, P = 0.0123 for the comparison between T-47D and SK-BR-3 and P...
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Báo cáo khoa học: Modulation of Ca2+ entry and plasma membrane potential by human TRPM4b pptx
... were then expanded in the same medium and analyzed for the expression of TRPM4b by RT-PCR and western blotting RT-PCR Total RNA was isolated, and RT-PCR was performed as described above The primer ... from the cellattached into the whole cell configuration The panels on the left show current development of inward currents at ) 100 mV and outward currents at + 80 mV against time The panels on the ... ⁄ Ca2+-readdition protocol was used to distinguish between the release of Ca2+ from intracellular stores and Ca2+ influx across the plasma membrane Whereas Ca2+ release did not significantly differ...
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Báo cáo khoa học: "Pharmacokinetics, tissue residue and plasma protein binding of ofloxacin in goats" pps
... seconds and then placed on ice for 15 to enhance precipitation It was centrifuged at 15,600 g for 10 and the supernatant (750 µl) was transferred to another tube Dichloromethane (6 ml) was added and ... After discarding the aqueous phase, the organic phase was transferred to a clean siliconized tube and evaporated to dryness at 40oC The residue was then reconstituted in mobile phase (500 µl) and ... monitored in Diode arry detector The chromatogram were integrated on the LC-100 laboratory computing integrator Plasma samples were subjected to liquid-phase extraction To ml of plasma, ml of methanol...
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Báo cáo y học: " Treatment outcomes and plasma level of ritonavir-boosted lopinavir monotherapy among HIV-infected patients who had NRTI and NNRTI failure" docx
... participated in the design of the study, statistical analysis and draft the manuscript SK, WA, WP, ST, KR and SS participated in the design of the study and draft the manuscript SN participated in the design ... integrase inhibitor and chemokine receptor antagonist, are neither available nor affordable Nevertheless, the goal of attaining undetectable plasma HIV-1 RNA is remain mandatory [3] To date, several ... at the HIV Netherlands-Australia-Thailand Clinical Research Laboratory, which is located at the Chulalongkorn Medical Research Center (Bangkok), by high-performance liquid chromatography This...
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Báo cáo y học: "Molecular characterization of hepatitis A virus isolates from environmental and clinical samples in Greece" pptx
... structure than other picornaviruses and thus is characterised by high resistance to the environment and is able to persist for extended periods on environmental surfaces [2] The incidence of ... % nucleotide identity of the nucleotide sequence of PAT73 isolate with the sequences of the other HAV strains of the study is shown on the right Discussion The hepatitis A virus still represents ... country, Tunisia, showed that all Tunisian strains belonged to genotype I with a greater presence of sub-genotype IA (98%) and 2% of sub-genotype IB [15] The hepatitis A vaccine is included in the Greek...
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Analysis of Pesticides in Food and Environmental Samples - Chapter 1 doc
... inhibitor fungicides are active against many different fungi Although they disturb sterol synthesis in higher plants, as well as the synthesis of gibberellins, their phytotoxicity is low The synthesis ... for building the intracellular skeleton in eukaryotic cells forming the wall of microtubules These compounds disturb normal cell division by binding with tubulin Inhibitors of cell division are ... tubulin, a protein that is the building block of the intracellular skeleton in cells The impairment of cell division is produced in most cases by inhibiting the formation of the microtubules Benzimidazoles,...
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Analysis of Pesticides in Food and Environmental Samples - Chapter 2 pdf
... Figure 2.8, the experimental procedure is very simple Firstly, the fiber is inside the protecting needle which is introduced into the sample vial Then, the fiber is exposed to the sample to perform extraction ... transfer is performed in one step, and it is possible to achieve high separation and concentration factors The distinguishing factor of the use of SLMs or MMLLE is the possibility of connecting them ... sample is placed in a glass mortar and blended with the sorbent until a complete disruption and dispersion of the sample on the sorbent is obtained Then, the mixture is directly packed into an...
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Analysis of Pesticides in Food and Environmental Samples - Chapter 4 ppt
... is a sigmoidal curve similar to the one shown in Figure 4.3 for the ELISA to detect 3-PBA Note that the detection range tends to be narrow using this format (due to the sigmoidal curve) and the ... solution and the inhibitor bound to the electrode surface The ability of cholinesterase to bind to the paraoxon immobilized on the electrode is minimized or prevented in the presence of free inhibitor ... assumes that the differences have a symmetric distribution In contrast, the sign test does not make this assumption and therefore does not require log transformations of the data The McNemar’s...
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Analysis of Pesticides in Food and Environmental Samples - Chapter 5 pdf
... planned, performed, monitored, archived, and reported The ISO=IEC 17025:2005 Standard, replacing the previous standards (ISO=IEC Guide25 and EN 45001), contains all the general requirements for the technical ... performed using standard methods, nonstandard methods, and laboratory-developed methods, and incorporates all those requirements of ISO 900 1and ISO 9002 that are relevant to the scope of the ... transport and storage Varying sample size The analytical sample is in contact and contaminated by other portions of the sample Rinsing, brushing is performed to various extent; stalks and stones...
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Analysis of Pesticides in Food and Environmental Samples - Chapter 8 pdf
... microwave-assisted extraction with 10 mL toluene and mL water and irradiated during Vessels are opened after cooling, the toluene extract is evaporated, and mL of hexane is added The hexane extract is ... at 1008C The sample is agitated with a magnetic stirring bar during the head space SPME The polyacrilate (PA) fiber is exposed to the headspace for 25 min, and then inserted in the injector of a ... from soil The application of MAE for the extraction of pesticide residues is increasing in the last years and together with other modern techniques, such as sonication and PLE, are the most widely...
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Analysis of Pesticides in Food and Environmental Samples - Chapter 9 doc
... containers and transported or shipped to a laboratory for extraction and analysis With SPE disks, it is possible to extract pesticides from water in the field and transport only the disks to the laboratory ... analyte into the sorbent phase of the fiber The fiber is then withdrawn, and introduced directly into a GC inlet to thermally desorb the enriched analyte into the GC column or eluted with the mobile ... that does not favor pesticide enrichment into the organic phase It is difficult to automate, and performance is likely dependent on the analyst’s skills The solvent chosen must be completely immiscible...
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Analysis of Pesticides in Food and Environmental Samples - Chapter 10 pdf
... conductivity sensor is activated when it starts to rain and then the lid on top of the funnel is removed At the end of the rain period the lid is again moved back onto the funnel With this system, no ... derivatization on the fiber or onto the GC injector For the direct technique, it is necessary to adjust the pH of the water below of the pKa of the molecules to be derivatized (i.e.,
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Analysis of Pesticides in Food and Environmental Samples - Chapter 11 pot
... milk,54,56,57 and meat.58–60 The levels are dependent on the age of the animals at the time of slaughter58 and the fat content of the product,59 that is, the older the animal and the higher the fat ... life and in the risk assessment this intake is compared with the ADI The acute intake or the short-term intake, on the other hand, is the intake within 24 h or less, for example, a meal The acute ... for the calculations of pesticide intakes The advantages of the approach are that the approach is easy and simple to perform and the results are easy to interpret The drawback of the approach is...
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Analysis of Pesticides in Food and Environmental Samples - Chapter 12 (end) potx
... for synergistic action, that is, the effect of the chemicals together is greater than that predicted from the parts, while the converse of this (antagonistic effect) is not likely to be of priority ... that is the time required for one-half of the pesticide to decompose to products other than the parent compound The longer a pesticide persists within the environment, the greater the risk it ... simple exposure and toxicity ratios and the second is a probabilistic approach in which the risk is expressed as the degree of overlap between the exposure and effects Apart from these methods,...
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báo cáo khoa học: " LysoPC acyltransferase/PC transacylase activities in plant plasma membrane and plasma membrane-associated endoplasmic reticulum" ppt
... We therefore had to invert the plasma membrane vesicles prior to the yeast protocol treatment of lowering the pH to separate the PAMs from the plasma membrane [12] The protein patterns of the ... fraction) and isolated plasma membrane (PM) The arrows to the right of the lanes point to differences between the PAM and PM fractions Otherwise as in Fig Page of 12 (page number not for citation ... and ASS designed the pea studies and KEL performed all isolations of and assays on pea membranes HT contributed with the confocal microscopy and the Golgi antibody studies KEL and ASS wrote the...
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Báo cáo khoa học: " Mass transfer, clearance and plasma concentration of procalcitonin during continuous venovenous hemofiltration in patients with septic shock and acute oliguric renal failure" ppsx
... correlated to the severity score and prognosis PCT seems to be this marker Nevertheless, in the case of alteration of the PCT concentration during renal replacement therapy, diagnostic and therapeutic ... in the blood and ultrafiltrate samples The samples were taken at the beginning of CVVH (T0) and at the following times, according to the kinetics of PCT and to the lowest degree of clotting formation ... clearance at T15′ therefore seems to be linked to the CVVH techniques but not to the inflammatory status The low variability of these results has also been confirmed at T60′ and T6h There was no significant...
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Investigation and application of liquid chromatography mass spectrometry in the analysis of polar, less volatile and thermal unstable organic pollutants in environmental and biological samples 6
... been applied to analyze the residues of N-methylcarbamate and related compounds (propoxur and its metabolisms as model compounds) in biological materials (goldfish, tortoises and sea lettuce) Orthogonal ... employed to optimize MAE conditions It is found that optimized conditions were different in different biological matrices The average recoveries of tested compounds in goldfish, tortoises and algal ... decomposition was observed during the process The extracts of goldfish, tortoises and sea lettuce were stable and a 3-days delay prior to analysis was possible 144 ...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose