... live in a snake.
14. My brother managed to kill the snake just at the time when I were almost exhausted.
Supply thecorrectformofthe verbs
1. Cats could fly if they (have) wings.
2. If Peter ... (not,want) to live in a snake.
14. My brother managed to kill the snake just at the time when I (be) almost exhausted. If
he (be) a little late, I (kill) by the snake.
15. Had I know you were ill, ... snake.
14. My brother managed to kill the snake just at the time when I (be) had been almost
exhausted. If he (be) had been a little late, I (kill) would have been killed by the snake.
15. Had...
... Give thecorrectformoftheverbs (Englsh 8)
1 .The sun always (rise) in the east. Look, it (rise)!
2. I don’t think I (go) out tonight. ... her homework yet.
28. Would you like some coffee? I just (make) some.
29. She (feed) the cat yet?
- Yes, she (feed) it half an hour ago.
30. The weather (become) colder and colder in December.
31. ... evening. I (stay) at home.
33. The film (begin) at 7.30 or 8.00?
34. You (be) away?
- Yes. I (go) to the country last Sunday.
35. They just (phone) to say that they (not come) back till Friday...
... biological function ofthe pro -form of
bone morphogenetic protein-2 (BMP-2), a member ofthe transforming
growth factor b (TGFb) ⁄ BLP superfamily. The role ofthe 263 amino acids
of the pro-peptide ... Furthermore, the pro-peptide
of GDF-8 impairs interaction ofthe mature part with
its receptors [16]. In the case of BMP-9, the pro-pep-
tide appears not to alter significantly the activity of the
mature ... interested in the role ofthe 263
amino acid pro-peptide of BMP-2, because evidence
accumulated over recent years has shown that the
pro-forms of growth factors can modulate the activities
of the mature...
... rates) prior to and after the transition ofthe enzyme to a
more active form, respectively, t is the time and s is the lag-
time. The rate constant, k, for the activation ofthe enzyme is
obtained ... lip prevented binding ofthe C-terminal
residues over the active site, or the absence of the
C-terminal residues caused the movement ofthe lip
(Fig. 1B). In addition, the C
a
chain between amino
acid ... between the dCTP(dUTP)-binding
conformer and the dTTP-binding conformer of dCTP
deaminase. We were not able to identify structural
changes in the main chain ofthe subunit, or in the
interaction of...
... the SH3 domain of Noxo1 participates in
regulation of Nox3, the role ofthe PX domain in Nox3
activity remains unknown.
In the process of cloning of human Noxo1, some
spliced transcripts ofthe ... To compare the amounts of
the two variants expressed in the testis, we performed
the PCR using the primers ‘d’ and ‘c’, where the
cDNAs for both Noxo1b and Noxo1c are amplified as
the insert region ... between the pair of primers
(Fig. 2A). The lengths ofthe PCR products of Noxo1b
and Noxo1c were 225 and 240 bp, respectively. To
delineate the difference in the two products, we subjec-
ted the...
... enzyme.
The catalytic mechanism of hydrolysis
The fructose present in the crystal structure of the
complex is the result of hydrolysis of raffinose. The
conformation ofthe sugar observed in the electron
density ... attack on
the anomeric carbon of fructose. The leaving group is
carboxylate of Asp54.
Dimerization
The asymmetric units ofthe crystals of both the apo
and complexed formofthe enzyme consist of dimers
with ... blades in the axis
area are of polar character. The water channel in the
apo formofthe enzyme runs along the axis of the
b-propeller through the whole domain and is only
blocked by Cys236 in the...
... between
the hypophosphorylated initiation isoform IIA and
the hyperphosphorylated elongating isoform IIO that
exhibits reduced electrophoretic mobility compared
with the IIA isoform. Whereas the ... replicases in the eukary-
otic cell [22]. Therefore, we tested the effect of the
replicative state ofthe cell on the interaction between
Pol e and RNA pol II. A possible confinement of the
interaction ... specifically in the G
1
phase of
the cell cycle. This effect could be attributed to inhi-
bition of transcription of cell cycle-regulated genes at
this phase ofthe cell cycle [51]. Nevertheless, it...
... regulation ofthe PKI
and PKI isoforms ofthe inhibitor protein ofthe cAMP-dependent
protein kinase. J. Biol. Chem. 272, 20011–20020.
20. Byler, D.M. & Susi, H. (1986) Examination ofthe secondary
structure ... Characterization of PKI
gamma, a novel isoform ofthe protein kinase inhibitor of cAMP-
dependent protein kinase. J. Biol. Chem. 272, 18169–18178.
4. Gamm, D.M. & Uhler, M.D. (1995) Isoform-specific ... showedasinglebandbySDS/PAGE
(Fig. 2). The assay of its activity demonstrated that the
purified PKIb inhibited the catalytic subunit of cAPK with
the specific activity of 6.0 · 10
4
unitÆmg
)1
(Fig. 3A) .The K
i
value determined...
... effect on the thermal
unfolding of proCPY.
The P
m
value for CPY was higher than that of its
unglycosylated form, though the DV and DG
P
values were
almost the same. The P
m
value of proCPY was ... the corresponding values of
proCPY and Dgly proCPY (Fig. 2B), indicating that the
precursor form was less thermally stable than the mature
form, regardless ofthe carbohydrate moiety.
After the ... unfolding ofthe unglycosylated
form.
At high pressure, in the glycosylated forms the carbohy-
drate moiety of CPY and proCPY would be hydrated to
compensate the volume contraction and the protein...
... iden-
tified as the major and the minor forms ofthe holo-
ACP structure, on the basis of their percentage contri-
butions (65% and 35%, respectively) to the overall
structure ofthe protein. The structures ... both forms have the same amino acid
sequence, and they have similar secondary structures, it was found that the
– DG of unfolding ofthe holo form was lower than that ofthe apo form at
all the ... unfolding
thermodynamic parameters ofthe two forms are given
in Tables 1–3. In the entire experimental regime, it is
seen that the holo form presents better stability than
the other form (Fig. 4C). The...